The Effect of the Host on Ultraviolet Inactivation and Photoreactivation of a Bacterial Virus

Friedman, Murray

doi:10.2307/3570566

Cited by 5 publications

(2 citation statements)

References 10 publications

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“…One point which is difficult to explain is that recognition of the PF sometimes differed among different cells [7,12,14,15,19,26,32]. Data at hand indicate that the PF of herpes virus requires a longer time for complete adsorption and penetration than native virus, similar to that observed in the case of poliovirus [17].…”

Section: Proof For the Presence Of Non-aggregate Unneutralizable Virusmentioning

confidence: 81%

Studies on the Neutralization of Herpes Simplex Virus

Yoshino

Morishima

1972

Japanese Journal of Microbiology

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A portion of the seed virus remained active even in the presence of excess antibody. With an early serum or relatively low concentrations of a hyperimmune serum, the presence of such unneutralizable virus was uncovered by the addition of complement. This unneutralizable persistent fraction (PF) was distinguished from sensitized virus surviving in insufficient antibody, because the latter was inactivated by a high concentration of antibody as quickly as the control virus. The level of the PF was constant regardless of the serum species, serum lot, dilution of serum, antibody class and complement requirement of antibody, being approximately 0.1% of the seed virus. Millipore filtration of the seed virus lowered this PF level to varying degrees depending upon the porosity. However, when filtered virus-serum mixtures were incubated at 37 C for a sufficient time and then filtered again, a portion of the unneutralized virus did pass a 0.22 membrane. Furthermore, virus sensitized with insufficient antibody showed no increase in resistance to complement after 10 hr incubation at 4 C. These results proved that viral aggregation was not the essential cause of the PF. It was confirmed, on the other hand, that the neutralization of virus by hyperimmune IgG followed a one-hit curve. Analysis of these data appeared to support Rappaport's postulation that the PF represented antibody-coated virus whose critical antigenic sites were all left unbound.

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Section: Proof For the Presence Of Non-aggregate Unneutralizable Virusmentioning

confidence: 81%

Studies on the Neutralization of Herpes Simplex Virus

Yoshino

Morishima

1972

Japanese Journal of Microbiology

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“…I t is reported t o be shortest a t the optimum temperature and nutrition of the host (6,7). Data on percentage adsorption and burst size fluctuate sonlewhat despite carefully conducted experiments (1,8).…”

Section: Growth Studiesmentioning

confidence: 99%

Growth Characteristics of Phages of Agrobacterium Radiobacter

Roslycky¹,

Allen²,

McCoy³

1965

Can. J. Microbiol.

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Four phages of A. mdiobacter, isolated from sewage and soil, r e q~~i r e d divalent ions for m~~ltiplication. Chelating agents inactivated free phage irreversibly. With citrate and phosphate the order of decreasing sensitivity of the phages was PR-590a > PS-192 > PsR-1012 > PR-1001. E D T i l was the most inhibitory. Phage growth was promoted by the adclition of 116of Caf+ or .\iIgFi in a divalent ion-free m e d i~~m . These ions were r e q~~i r c d for the adsorption step in the gro\\.th cycle of phage PI<-1001. Latent periocls of the four phages in nutrient broth ranged from 35 to 37 minutes for PsR-1012 and 42 to 45 minutes for Pit-590a. Burst sizes \\.ere 65, 27, 56, and 69, and percentage adsorptions after 5 ~n i n~l t c s were 35, 12, 36, and 43 for PR-1001, 1's-192, PIZ-590a, arid Psi<-1012, respectively. The optimum temperature for adsorption and yield of phage PS-192 was 25 OC while the shortest latent period o c c~~r r e d a t 30-32 'C. =It 35 OC this phage failed to m~~l t i p l y .'Published with the approval of the Director of the \Visconsin Agric~llt~~ral Experiment Station, AIadison. I'art of this work was carried out a t

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