The effect of the sucrose concentration on the initiation and growth of adventitious buds from leaf tissue of Lachenalia

The relationship between genotype, tissue age and endogenous cytokinin levels on adventitious bud formation on leaves of Lachenalia

“…It has been reported that the response of different hybrids and tissue from different positions on the leaf varies greatly [14].…”

In Vitro Rooting and Greenhouse Acclimatization of Lachenalia Shoots

“…Lachenalia species can be propagated from seed and bulb cuttings, and some from leaf cuttings (Duncan, 1988) and through tissue culture (Klesser and Nel, 1976;Nel, 1983;Niederwieser and Vcelar, 1990). The effects of explant-tissue age and orientation, sucrose, BA, and NAA on adventitious bud formation from leaf tissue have been investigated (Niederwieser and van Staden, 1992;van Staden and Drewes, 1994;van Rensburg and Vcelar, 1989). Klesser and Nel (1976) and Nel (1983) have published limited information on the in vitro rooting and greenhouse acclimatization of Lachenalia tissue-culture shoots.…”

Actual, potential and speculative applications of seaweed cellular biotechnology: some specific comments on Gelidium

“…Sugars have been reported to be unnecessary or even inhibitory to tissue (Fucus spiralis: Fries, 1984), callus (Ecklonia radiata: Lawlor et al, 1989) and cell culture (Porphyra umbilicalis: Tait et al, 1990) growth or organogenesis. Our studies on the interrelationship between the osmolality of the culture medium and the effect of osmotically active sugar supplementation has shown that, as in higher plants (van Rensburg & Vcelar, 1989), the effects of carbohydrates in seaweed tissue and callus culture seem osmotic or metabolic or both (Robaina et al, 1990a;1990b). Solidity of culture media promotes disorganization (Robaina et al, 1990a;1990b).…”