The effect of transplacental administration of ddt on organ cultures of foetal mouse lung tissue

Self Cite

DDT must be evaluated as a weak carcinogen.One of the most widely used pesticides, DDT, continues to be a subject of increasing interest. The first experimental observation on the blastomogenic action of DDT in rats was made by Fitzhugh and Nelson in 1947. Since then reports on the carcinogenic effect of DDT in experiments have multiplied (Kemeny and Tarjan, 1966;Halver, 1967; Fitzhugh, 1969; Innes et al., 1969;Tarjan and Kemeny, 1969). The tumours that appeared in the treated animals were principally hepatomas, lung adenomas and lymphomas. However, in some experiments the blastomogenic effect of DDT was not substantiated (Durham, 1963 ; correspondence of Terracini and Tarjan, 1970;Agthe et al., 1970).In our laboratory it has been shown that diffuse and focal proliferation, adenomatous focal growth and, later, adenomas developed in lung organ cultures of foetal mice, rats and golden hamsters, the mothers of which had been treated with urethane, blastomogenic nitrosamines or hydrocarbons during the final period of pregnancy (Kolesnichenko, 1966(Kolesnichenko, , 1971(Kolesnichenko, , 1972 Smetanin, 1969;Shabad et al., 1971). The sameexperimental model was used to evaluate the possible carcinogenic effect of DDT.Organ cultures of foetal lung tissue from strain A mice exposed to DDT through the transplacental route showed intensive growth as compared to controls. A dose of 50 ppm of DDT induced epithelial preadenomatous lesions (diffuse hyperplasia and focal proliferation) in the first generation (F,) in 32.2 %. A progressively higher incidence of these lesions was observed at a dose level of 10ppm in F1 (8.9%), F, (16.4%) and F, (39.3 %) generations. However, the incidence dropped in the F4 (30.1%) and FS (20.1%) generations (Shabad et al., 1972). This paper deals with the results of a parallel study in vivo of the effect of DDT, especially on lung turnorigenesis in the same five generations of mice. In these multigeneration experiments we attempted to compare our results in organ culture in vitro and in mice in vivo, and evaluate the possible blastomogenic effect of the pesticide DDT.

mentioning

confidence: 98%

Transplacental and combined long‐term effect of DDT in five generations of a‐strain mice

Shabad¹,

Kolesnichenko²,

Nikonova³

1973

Self Cite

“…The transplacental effect of some carcinogenic substances on organ cultures from mice, rats and hamsters was demonstrated in previous papers (Kolesnichenko, 1966(Kolesnichenko, , 1971(Kolesnichenko, , 1972(Kolesnichenko, , 1975Shabad and Golub, 1973;Shabad et al, 1971 ;Shabad et al, 1972a;Shabad et al, 19726;Shabad et al, 1975;. However, organ culture of human tissues has received less attention and thus far only Lasnitzki (Lasnitzki, 1956(Lasnitzki, , 1964 has studied the effect of benz(a)pyrene, methylcholanthrene and cigarette smoke condensates on organ cultures of human embryonic lung and skin.…”

mentioning

confidence: 68%

“…

The transplacental effect of some carcinogenic substances on organ cultures from mice, rats and hamsters was demonstrated in previous papers (Kolesnichenko, 1966(Kolesnichenko, , 1971(Kolesnichenko, , 1972(Kolesnichenko, , 1975Shabad and Golub, 1973;Shabad et al, 1971 ;Shabad et al, 1972a;Shabad et al, 19726; Shabad et al, 1975;. However, organ culture of human tissues has received less attention and thus far only Lasnitzki (Lasnitzki, 1956(Lasnitzki, , 1964 has studied the effect of benz(a)pyrene, methylcholanthrene and cigarette smoke condensates on organ cultures of human embryonic lung and skin.

This paper deals with an attempt to use organ cultures of human embryonic lung and kidney tissues for studying the action produced by the known carcinogens N-nitrosomethylurea (NMU), dimethylnitrosamine (DMNA) and orthoaminoazotoluene (OAAT).

MATERIAL A N D METHODS

Lung and kidney tissues of 22-to 24-week-old human embryos were cultivated according to earlier described methods (Kolesnichenko, 1966;Shabad et al, 1971;Shabad et al, 19726).

…”

mentioning

confidence: 73%

The effect produced by some carcinogenic nitrosocompounds on organ cultures from human embryonic lung and kidney tissues

Shabad¹,

Kolesnichenko²,

Golub³

1975

Self Cite

Previous works using organ cultures of lung and kidney tissues of mouse, rat and hamster embryos exposed to transplacental action of some carcinogens showed premalignant growth. This paper presents the results of organ culture of human embryonic lung and kidney tissues. Direct action of some carcinogenic nitrosocompounds (N-nitrosomethylurea, dimethylnitrosamine and orthoaminoazotoluene) resulted in higher survival of the treated organ cultures and induced epithelial hyperplasia. The changes depended directly upon the dose of carcinogen employed.

The effect of long‐term exposure to DDT on CF‐1 mice

Tomatis¹,

Turusov²,

Day³

et al. 1972

122

CF-I minimal inbred mice were given technical DDT mixed into the diet atThe main reasons for the concern regarding the potential hazard for human health represented by DDT are based on (a) its ubiquity, (b) its persistence in the environment, (c) its capacity to accumulate in living organisms, including man, and its capacity to accumulate in the foetus and in mothers' milk and (d) the experimental evidence of its ability to increase tumour incidence in laboratory animals.The experimental evidence of the carcinogenicity of DDT is based on five published reports. Fitzhugh and Nelson (1947) reported the occurrence of hepatic cell tumours in four of 156 Osborne Mendel rats receiving DDT (81.8% pp isomer and 18.2% op isomer) in the diet at levels of 200-800 ppm, while 1 1 other rats were described as showing nodular adenomatoid hyperplasia of the liver. All 15 rats survived over 18 months and the age distribution for rats with liver tumours corresponded to that in untreated controls. Hepatomas occurred in about 1% of the untreated controls. KemCny and Tarjan (1966) reported in a preliminary paper that the administration of DDT (crystalline pp'DDT) at levels of 2.8 and 3.0 ppm in the diet to BALB/c mice for five consecutive generations resulted in a progressively increased incidence of tumours from the second generation onwards. Tarjan and KemCny (1969) published a final report on this experiment, from which it appeared that the total incidence of tumours was higher in the DDT-treated groups of all five generations than in the control group. The most frequent tumour types were leukaemia, reticulum-cell sarcoma, carcinoma of the lungs and haemangioendothelioma. A transgeneration cumulative effect, resulting in a progressive increase in tumour incidence from the second generation onwards, was not substantiated in this report (Terracini and Tarjan, 1970).Halver ( 1967) reported the occurrence of hepatomas in rainbow trout fed a diet of 18 or 75 ppm DDT. Innes et al. (1969) reported the occurrence of hepatomas in mice of two strains following the continuous administration of DDT for 80 weeks. DDT (pp'DDT) was given