The effect of triacontanol on shoot multiplication and production of antioxidant compounds in shoot cultures of Salvia officinalis L.

Grzegorczyk, Izabela; Bilichowski, Ireneusz; Mikiciuk-Olasik, Elżbieta; Wysokińska, Halina

doi:10.5586/asbp.2006.002

Cited by 18 publications

(28 citation statements)

References 15 publications

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“…At this time, on average, 3 shoots per explant were formed and the multiplication rate in the following ten subcultures under the described conditions remained at the same level. Also on the agargelled medium with the same growth regulators, sage cultures responded with average number of 3 shoots per explant, but the time required for the shoot induction was longer (5 weeks) (Grzegorczyk et al 2006). With respect to morphology, sage shoots grown in liquid medium were characterized by higher number of leaves, larger leaf areas and shorter internodes compared to those cultured on the agar-solidified medium (data not shown), and a decrease in the average shoot length in the liquid medium was observed.…”

Section: Resultsmentioning

confidence: 99%

“…TRIA solution was prepared as described in our previous work (Grzegorczyk et al 2006). After 3 weeks, the number of shoots per explant was determined.…”

Section: Influence Of Triamentioning

confidence: 99%

“…The solvent systems were acetonitrile/0.1% o-phosphoric acid 65:35 (v/v) and acetonitrile/0.1% o-phosphoric acid 30:40 (v/v) for diterpenes and RA analysis, respectively. The details of extraction procedure and HPLC analysis were described earlier (Grzegorczyk et al 2006). The compound concentrations were achieved using calibration curves prepared with standard compounds.…”

Section: Extraction and Quantitative Determination Of Antioxidant Commentioning

confidence: 99%

“…In this study, triacontanol (TRIA) was chosen as the factor. The choice of TRIA was based on our earlier research with sage shoots cultured on agar medium supplemented with this natural plant growth promoter (Grzegorczyk et al 2006) and literature reports (Yaseen and Tajuddin 1998;Tantos et al 1999;Fraternale et al 2003). For comparison, contents of antioxidant compounds in commercially available samples of sage leaves were presented.…”

Section: Introductionmentioning

confidence: 99%

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Liquid shoot culture of Salvia officinalis L. for micropropagation and production of antioxidant compounds; effect of triacontanol

Grzegorczyk

Wysokińska

2011

Acta Soc Bot Pol

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Liquid shoot culture of Salvia officinalis L. in MS medium containing IAA (0.1 mg l -1 ) and BAP (0.45 mg l -1 ) was developed and evaluated in relation to shoot multiplication and antioxidant compound (carnosic acid, carnosol and rosmarinic acid) accumulation. In the liquid medium, on average, 3 new shoots per explant (shoot tip) were obtained within 3 weeks. The shoots produced 8.2±0.02 mg of diterpenoids and 31.2±0.29 mg of rosmarinic acid per gram of dry weight. Shoot proliferation and diterpenoid content increased when triacontanol (5, 10 or 20 µg l -1 ) was added to the liquid medium. In optimum conditions (at 20 µg l -1 TRIA) almost 7 shoots were formed per explant after 3 weeks. An increase in diterpenoid production (expressed as the sum of carnosol and carnosic acid) ranged from 30% to 50% and dependended on triacontanol concentration tested. The level of diterpenoids in triacontanol-treated shoots was similar to the content of compounds in commercial herbal product (dried leaves of S. officinalis) (10-12 mg g -1 dry wt). Triacontanol did not increase rosmarinic acid production, but the content of the phenolic as compound in shoots grown in liquid culture (31 mg g -1 dry wt) was even 24 times higher compared to samples of dried leaves of S. officinalis plants. We also demonstrated that the highest amounts of CA, Car and RA were accumulated in young, top parts of sage shoots. This observation could be useful for improving the selection of material for the extraction of natural antioxidants from S. officinalis.

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Section: Resultsmentioning

confidence: 99%

“…TRIA solution was prepared as described in our previous work (Grzegorczyk et al 2006). After 3 weeks, the number of shoots per explant was determined.…”

Section: Influence Of Triamentioning

confidence: 99%

Section: Extraction and Quantitative Determination Of Antioxidant Commentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Liquid shoot culture of Salvia officinalis L. for micropropagation and production of antioxidant compounds; effect of triacontanol

Grzegorczyk

Wysokińska

2011

Acta Soc Bot Pol

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“…However, the use of TRIA concentrations (10 or 20 mg L Á1 ) did not induce further increase in the diterpenoid yield of sage (Salvia officinalis) shoots. Grzegorczyk et al (2006) observed a positive effect of TRIA on shoot multiplication, production of biochemical compounds, and antioxidant capacity of S. officinalis. Moreover, TRIA also increased carnosol content in sage shoots, with a little influences on carnosic acid.…”

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confidence: 90%

Triacontanol: a potent plant growth regulator in agriculture

Naeem

Khan

Moinuddin

2012

Journal of Plant Interactions

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Triacontanol (TRIA) is a natural plant growth regulator found in epicuticular waxes. It is used to enhance the crop production in millions of hectares, particularly in Asia. Quite a number of researchers have reported the TRIA-mediated improvement in growth, yield, photosynthesis, protein synthesis, uptake of water and nutrients, nitrogen-fixation, enzymes activities and contents of free amino acids, reducing sugars, soluble protein, and active constituents of essential oil in various crops. Expectedly, TRIA enhances the physiological efficiency of the cells and, thus, exploits the genetic potential of plant to a large extent. In fact, TRIA increased free amino acids, reducing sugars, and soluble protein of rice (Oryza sativa L.) and maize (Zea mays L.) within 5 min. TRIA elicited the appearance of L(')-adenosine within 1 min in the roots of plants, the shoots of which were sprayed with nanomolar concentrations of TRIA. TRIA and octacosanol (OCTA), the primary alcohols, are ubiquitous in the environment. OCTA was reported to inhibit the activity of TRIA in the seedlings of rice (Oryza sativa L.) at equimolar concentrations; and both TRIA and OCTA elicited a second messenger, known as OCTAM and triacontanol second messenger (TRIM), respectively. TRIA rapidly increases the ratio of L(')-to D(Á)-adenosine, probably at the tonoplast. However, it is to be resolved as to how TRIA elicits L(')-adenosine and what is the source of L(')-adenosine in plants. Based on known metabolic processes, de novo synthesis of L(')-adenosine is unlikely, because of the rapidity of the response. TRIA-mediated increase in dry matter production could influence the inter-relationship between primary and secondary metabolism, leading to increased biosynthesis of secondary products. Various studies present strong evidences that application of TRIA applied either to the root medium or to leaves enhanced the growth and yield of vegetables and other crops, including agronomic and horticultural crops as well as medicinal and aromatic crop plants under normal and adverse conditions. However, further investigations are required to elucidate the possible role of TRIA on plant growth regulation, physiological activities and secondary metabolite biosynthesis regarding medicinal and aromatic plants subjected to abiotic stress. The present review covers the pivotal role of TRIA in plant growth and development, its mode of action and its significance in improving the crop productivity and quality of agricultural crops.

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