The effect of trimethylamine N-oxide on the metabolism of visceral white adipose tissue in spontaneously hypertensive rat

He, Guangbin; Liu, Xiaocong; Hou, Xing-Hua; Feng, Yingqing

doi:10.1080/21623945.2022.2104783

Cited by 3 publications

(1 citation statement)

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“…TMAO is an important component of the metabolites of the intestinal flora and is one of the major uremic toxins [29]. Approximately 95% of circulating TMAO is excreted in the urine via the kidneys.…”

Section: Discussionmentioning

confidence: 99%

Urine and serum metabolomics study of wild ginseng in rats with spleen‐qi deficiency using rapid resolution liquid chromatography coupled with quadrupole‐time‐of‐flight mass spectrometry

Zhang

et al. 2023

J of Separation Science

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Patients with a spleen-qi deficiency often exhibit dysfunction in the metabolic system. Metabolites are considered the most direct reflection of individual physiological and pathological conditions and represent attractive candidates to provide deep insights into disease phenotypes. This study examines the potential therapeutic mechanism of wild ginseng on spleen-qi deficiency through the analysis of serum and urine metabolomics using rapid-resolution liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry. The reasons for the superiority of wild ginseng treatment over cultivated ginseng were also analyzed in depth. After wild ginseng intervention, anandamide, urobilinogen, aldosterone, and testosterone glucuronide were significantly reduced in serum. Meanwhile, argininosuccinic acid, L-cysteine, and seven other metabolites were significantly elevated in serum. Nine metabolites, including Lacetylcarnitine, and citrulline were elevated in the urine, and trimethylamine N-oxide, adrenic acid, and 10 other metabolites were reduced. Arginine biosynthesis, pantothenate and CoA biosynthesis, thiamin metabolism, taurine, and tryptophan metabolism pathways were mainly improved. Further analysis was conducted on the relationship between Lactobacillus and Akkermansia bacteria with metabolites, and it was found that they are mainly related to amino acid metabolites. This study provides strong theoretical support and direction for further explanation of the immune mechanism of wild ginseng and lays the foundation for future studies.

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Section: Discussionmentioning

confidence: 99%

Urine and serum metabolomics study of wild ginseng in rats with spleen‐qi deficiency using rapid resolution liquid chromatography coupled with quadrupole‐time‐of‐flight mass spectrometry

Zhang

et al. 2023

J of Separation Science

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Crosstalk between Atrial Cardiomyocytes and Epicardial Adipose Tissue in Atrial Fibrillation: Insights from Machine Learning Methods and Rat Atrial Fibrillation Model

Zhang,

Miao,

Song

et al. 2024

Preprint

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Objective: Investigating the Role of Epicardial Adipose Tissue as a Catalyst for Reversal and Reconstruction of Atrial Myocardial Cells in the Context of Dialogue with Atrial Myocardial Cells. Implications for Breakthroughs in Preventing Paroxysmal Atrial Fibrillation Progression. Methods: We obtained three datasets (GSE41177, GSE31821, and GSE135455) associated with atrial fibrillation (AF) from the Gene Expression Omnibus (GEO) database, which were subsequently merged for comprehensive analysis. Differentially expressed genes (DEGs) were identified using the "limma" package in the R software. Candidate AF genes were selected through machine learning techniques, including the LASSO regression algorithm and SVM-RFE algorithm. The diagnostic efficacy of these genes was evaluated using Receiver Operating Characteristic (ROC) curves. Additionally, CIBERSORT was employed to investigate the proportions of infiltrating immune cells in each sample, while the Pearson method was applied to examine the correlation between genes and immune cells. Further validation of the DEGs were performed by PCR in atrial fibrillation rats. Results: A total of 310 Differentially Expressed Genes (DEGs) were identified in atrial cardiomyocytes with epicardial adipose tissue. Using the LASSO regression and SVM-RFE algorithms, ID1, SCN4A, COL4A5, COLEC11, and SNAI2 were pinpointed as key genes associated with Atrial Fibrillation (AF). In both the training and validation datasets, these genes exhibited robust effectiveness. The immune infiltration analysis revealed that, in comparison to sinus rhythm (SR), atrial samples from patients with AF exhibited higher levels of neutrophils, while T cells follicular helper were relatively lower. Correlation analysis highlighted significant associations between ID1, SCN4A, COL4A5, COLEC11, SNAI2, and infiltrating immune cells. The outcomes of the RT- qPCR analysis in our investigation were consistent with the findings of bioinformatics analysis. Conclusions: In summary, this study posits that ID1, SCN4A, COL4A5, COLEC11, and SNAI2 emerge as pivotal genes in Atrial Fibrillation (AF), exhibiting correlation with infiltrating immune cells. Furthermore, it underscores the indispensable roles played by infiltrating immune cells in the context of AF.

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Low-Molecular-Weight Compounds Produced by the Intestinal Microbiota and Cardiovascular Disease

Cuervo,

McAlpine,

Olano

et al. 2024

IJMS

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Cardiovascular disease is the main cause of mortality in industrialized countries, with over 500 million people affected worldwide. In this work, the roles of low-molecular-weight metabolites originating from the gut microbiome, such as short-chain fatty acids, hydrogen sulfide, trimethylamine, phenylacetic acid, secondary bile acids, indoles, different gases, neurotransmitters, vitamins, and complex lipids, are discussed in relation to their CVD-promoting or preventing activities. Molecules of mixed microbial and human hepatic origin, such as trimethylamine N-oxide and phenylacetylglutamine, are also presented. Finally, dietary agents with cardioprotective effects, such as probiotics, prebiotics, mono- and poly-unsaturated fatty acids, carotenoids, and polyphenols, are also discussed. A special emphasis is given to their gut microbiota-modulating properties.

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The effect of trimethylamine N-oxide on the metabolism of visceral white adipose tissue in spontaneously hypertensive rat

Cited by 3 publications

References 64 publications

Urine and serum metabolomics study of wild ginseng in rats with spleen‐qi deficiency using rapid resolution liquid chromatography coupled with quadrupole‐time‐of‐flight mass spectrometry

Urine and serum metabolomics study of wild ginseng in rats with spleen‐qi deficiency using rapid resolution liquid chromatography coupled with quadrupole‐time‐of‐flight mass spectrometry

Crosstalk between Atrial Cardiomyocytes and Epicardial Adipose Tissue in Atrial Fibrillation: Insights from Machine Learning Methods and Rat Atrial Fibrillation Model

Low-Molecular-Weight Compounds Produced by the Intestinal Microbiota and Cardiovascular Disease

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