The effect of tumor necrosis factor (TNF)-α to induce matrix metalloproteinase (MMPs) from the human dental pulp, gingival, and periodontal ligament cells

Rhim, Eun-Mi; Park, Sanghyuk; Kim, Duck-Su; Kim, Sun‐Young; Choi, Kyoung‐Kyu; Choi, Gi-Woon

doi:10.5395/jkacd.2011.36.1.26

Cited by 3 publications

(3 citation statements)

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“…IL-1 β is considered to be a key mediator that promotes a variety of innate immune processes and exacerbates damage during chronic disease and acute tissue injury [ 4 , 37 , 41 ]. TNF- α also induces the activation of inflammatory cells, further increases the inflammatory response, and increases MMP-1 expression in pulp tissue [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Effects of Melatonin and 5-Methoxytryptophol on Lipopolysaccharide-Induced Acute Pulpitis in Rats

Basmacı¹,

Aksoy²,

Sebai³

et al. 2021

BioMed Research International

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Aim. The aim of this study was to investigate the possible therapeutic impacts of two pineal hormones, melatonin and 5-methoxytryptophol (5-MTX), in a rat model of acute pulpitis by analyzing biochemical and histopathological parameters. Methods. This research was done using 32 male and female Wistar albino rats with weight between 200 and 250 g. The rats were randomly divided into four groups: a control group (rats without any treatment), acute pulpitis (AP) group, AP+melatonin group, and AP+5-MTX group. In the AP-induced groups, the crowns of the upper left incisors were removed horizontally. Lipopolysaccharide solution was applied to the exposed pulp tissue before the canal orifices were sealed with a temporary filling material. Melatonin (10 mg/kg) and 5-MTX (5 mg/kg) were administered intraperitoneally. The rats were sacrificed 24 hours after pulp injury, and trunk blood and pulp samples were collected. The concentrations of TNF-α, IL-1β, MMP-1, and MMP-2 in sera and pulp samples were determined using ELISA assay kits. Results. TNF-α, IL-1β, MMP-1, and MMP-2 levels in the serum and pulp tissues were considerably higher in the AP group than the control group ( p < 0.01 ‐ 0.001 ). In the AP+melatonin and AP+5-MTX groups, TNF-α, IL-1β, MMP-1, and MMP-2 levels in the serum and pulp tissues were significantly lower than in the AP group ( p < 0.05 ‐ 0.001 ). Conclusions. Both melatonin and 5-MTX provided protective effects on acute pulpitis, which indicates they may be promising as a therapeutic strategy for oral disease.

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Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Effects of Melatonin and 5-Methoxytryptophol on Lipopolysaccharide-Induced Acute Pulpitis in Rats

Basmacı¹,

Aksoy²,

Sebai³

et al. 2021

BioMed Research International

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“…A number of studies have demonstrated the role of neuropeptides in mediating the levels of MMPs [45] , [46] . Transcripts (mRNA) for neuropeptides have been found in both dissected ciliary processes and cultured ciliary epithelial cells by Northern blot analysis and RT-PCR.…”

Section: Discussionmentioning

confidence: 99%

Cross-Talk between Ciliary Epithelium and Trabecular Meshwork Cells In-Vitro: A New Insight into Glaucoma

Lerner

Beit‐Yannai

2014

PLoS ONE

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PurposeIt is assumed that the non-pigmented ciliary epithelium plays a role in regulating intraocular pressure via its neuroendocrine activities. To test this hypothesis, we investigated the effect on a human trabecular meshwork (TM) cell line (NTM) of co-culture with a human non-pigmented ciliary epithelium cell line (ODM-2).MethodsThe cellular cross-talk between ODM-2 and NTM cells was studied in a co-culture system in which the two cell types were co-cultured for 5 to 60min or 2, 4 and 8h and then removed from the co-culture and analyzed. Analyses of the ERK and p38 mitogen-activated protein kinase (MAPK) pathways and of the activity of TM phosphatases and matrix metalloproteins (MMPs) were performed. Acid and alkaline phosphatase activity was determined by the DiFMUP (6, 8-difluoro-4-methylumbelliferyl phosphate) assay. MMP levels were determined by gelatin zymography.ResultsExposure of NTM cells to ODM-2 cells led to the activation of the MAPK signal transduction pathways in NTM cells within 5min of co-culture. Phosphorylation of ERK1/ERK2 and p38 peaked at 10 and 15min and then decreased over time. Interaction between ODM-2 and NTM cells promoted the expression of MMP-9 in the NTM cells after 4h of co-culture.ConclusionsOur findings provide support for the hypothesis that crosstalk does indeed take place between ODM-2 and NTM cells. Future studies should be designed to determine the relationship between the MMP system, MAPK kinases and phosphatases. Manipulation of these signaling molecules and the related NTM signal transduction pathways may provide targets for developing improved treatments for glaucoma.

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“…8 Kadar TNF-α di jaringan periodontal secara berlebihan akan merangsang sintesis matriks metaloproteinase (MMPS) sehingga terjadi degradasi matriks ekstraseluler gingiva. 9…”

Section: Pendahuluanunclassified

Tumor Necrosis Factor-Alpha a Potential Therapeutic Target for Periodontitis and Diabetes Mellitus

Widjaja,

Syaify,

Hendrawati

2023

interdental

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Introduction: Diabetes mellitus (DM) is a systemic disease that affects many human populations in the world. One of DM’s oral complications is periodontitis. The pro-inflammatory cytokine that plays a major role in the relationship between these two diseases is TNF-α. Review: Diabetes mellitus is a systemic disease characterized by high blood glucose levels (hyperglycemia) due to inadequate insulin secretion, insulin resistance or both. Metabolic disorders caused by hyperglycemia can cause endothelial dysfunction in blood vessels. Endothelial dysfunction that occurs due to hyperglycemia can trigger an inflammatory response that can increase the progression of periodontitis. Periodontitis is a chronic inflammatory response in periodontal tissue to pathological microorganisms in dental biofilm. The periodontitis process begins with gingival inflammatory response. The initial phase of gingival inflammation is characterized by redness and bleeding on probing. Worsening inflammation will cause loss of periodontal tissue attachment and alveolar bone resorption which can lead to tooth loss. The inflammatory process that occurs in periodontitis involves pro-inflammatory cytokines that can influence insulin resistance, such as Tumor necrosis factor-alpha (TNF-α). Tumor necrosis factor-alpha is also a cytokine that plays a major role in the pathogenesis of diabetes mellitus, so that periodontitis and diabetes mellitus are interrelated diseases Conclusion: The TNF-α cytokine is a potential therapy target for periodontitis and diabetes mellitus.

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The effect of tumor necrosis factor (TNF)-α to induce matrix metalloproteinase (MMPs) from the human dental pulp, gingival, and periodontal ligament cells

Cited by 3 publications

References 36 publications

Anti-Inflammatory Effects of Melatonin and 5-Methoxytryptophol on Lipopolysaccharide-Induced Acute Pulpitis in Rats

Anti-Inflammatory Effects of Melatonin and 5-Methoxytryptophol on Lipopolysaccharide-Induced Acute Pulpitis in Rats

Cross-Talk between Ciliary Epithelium and Trabecular Meshwork Cells In-Vitro: A New Insight into Glaucoma

Tumor Necrosis Factor-Alpha a Potential Therapeutic Target for Periodontitis and Diabetes Mellitus

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