The Effect of Ultraviolet Radiation on Chlorophyll in Chlamydomonas reinhardtii

Nassour, Rana; Ayash, Abdulkarim; Mohamad, Issam

doi:10.14445/23942568/ijaes-v4i6p105

Cited by 3 publications

(2 citation statements)

References 18 publications

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“…The collected cells were treated by cell-disruption method shown as above. To measure the quantities of chlorophyll a/b, data of Abs 665 and Abs 652 of the collected solution were obtained after subtracting Abs 750 for a baseline correction using PBS with a spectrophotometer U-2900 (Hitachi, Tokyo, Japan) and substituted in the formula as below, chlorophyll a (µg·mL −1 ): 16.72 × Abs 665 – 9.16 × Abs 652 ; chlorophyll b (µg·mL −1 ): 34.09 × Abs 652 – 15.28 × Abs 665 (Nassour et al 2017 ). The contents of chlorophyll a/b in the cells were finally shown using the unit µg·mg-DCW −1 .…”

Section: Methodsmentioning

confidence: 99%

Fabrication of cell plastics composed only of unicellular green alga Chlamydomonas reinhardtii as a raw material

Nakanishi

Iritani

Tsuruta

et al. 2022

Appl Microbiol Biotechnol

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Cell plastics in this study were fabricated with only unicellular green alga Chlamydomonas reinhardtii as raw materials. The sizes of cell-major axis as structures were 8.4 ± 1.2 µm, and the aspect ratios of those were 1.2 ± 0.1, showing homogeneous particle size. After optimizing extraction condition of intracellular contents, cell plastics were fabricated with the cells as ingredient components and the intracellular contents as matrix components. Those cell plastics were observed with scanning electron microscopy, displaying the smooth surfaces of the cell plastics at a low magnification level. However, the surface, especially exposed surface, were rough at high magnification level. Tensile strength test revealed that increasing the ratio of intracellular contents in the cell plastics until 21% led enhancing mechanical properties of Young’s modulus and tensile strength; however, 25% of intracellular contents displayed decreases of those properties. As the optimal point, the cell plastic (21%), which contained 21% (w/w) of intracellular contents in cell plastics, showed 764 ± 100 MPa and 8.6 ± 5.2 MPa of Young’s modulus and tensile strength. The cell plastics showed few plastic region and soon fractured, indicating the possibility that cells and intracellular contents could be electrostatically connected. Additionally, cells were shown as a negative charge and displayed the possibility to contribute electrically cell-gathering with intracellular ionic components. Therefore, cells and intracellular contents containing ionic metabolites could be electrostatically connected for giving the mechanical strength to cell plastics. In this study, we successfully demonstrated fabricating cell plastics with only cells for the first time and also showed the high possibility of conjugating each cell with the intracellular contents. Key points • Cell plastics are fabricated with unicellular green algal cell directly. • Unicellular cells required to be conjugated for the fabrication with matrix. • Cells were conjugated with intracellular contents for cell-plastic fabrication.

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Section: Methodsmentioning

confidence: 99%

Fabrication of cell plastics composed only of unicellular green alga Chlamydomonas reinhardtii as a raw material

Nakanishi

Iritani

Tsuruta

et al. 2022

Appl Microbiol Biotechnol

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“…Cahaya dengan panjang gelombang relatif pendek memiliki energi radiasi lebih tinggi. Klorofil lebih sensitif terhadap radiasi UV-C dibanding UV-B, sehingga dampaknya lebih merusak (Nassour et al, 2017). Menurut penelitian yang telah dilakukan oleh Lung et al (2022), radiasi UV-C dapat mengakibatkan tingkat kematian sampai 91,76 ± 3,33% pada kultur Chlorella sp.. Cahaya UV menghasilkan radiasi gelombang elektromagnetik yang lebih tinggi energinya dibandingkan dengan PAR.…”

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Peningkatan Kadar Fenolik Total dari Chlorella sp. Menggunakan Cekaman Radiasi Ultraviolet-B

2023

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The treatment of abiotic stressing in microalgae cultures can trigger the production of secondary metabolites that have a variety of bioactivity. Phenolic compounds are one of the secondary metabolites produced by Chlorella sp. and have been categorized as an important antioxidants that play a role in the scavenging of free radicals. This research uses ultraviolet (UV)-B (λ 280-320 nm) as a stressing in Chlorella sp. cultures. The aim is to determine the effect of UV-B radiation on the biomass production of Chlorella sp. and total phenolic content (TPC), as well as its antioxidant activity. The research method used is experimental with a completely randomized design (RAL). The treatment tested was UV-B radiation with different duration of 0 (control), 40, 80, and 120 minutes/day during the culture period. Chlorella sp. culture is carried out using 12 glass containers with seawater (salinity 35 ppt) as culture media and enriched by Walne fertilizer (1 mL/L). During the culture period, photosynthetically active radiation (PAR) light and aeration are carried out for 24 hours. Biomass of Chlorella sp. was harvested 1 day after the peak of growth, then determined the weight of biomass and ethanol extract, followed by TPC, and antioxidant activity. The TPC test was carried out using the Follin-Ciocalteu reagent, while antioxidant activity used an inhibition test against 2,2-diphenyl-1-picrylhydrazyl (DPPH). The results showed that UV-B radiation had no significant effect on the biomass (p=0.122) and its ethanol extract yield (p=0.194), but had a significant effect on TPC (p=0.003) and DPPH inhibition percentage (p=0.036). The UV-B radiation for 80 minutes/day for 10 days of the Chlorella sp. culture can be used to increase phenolic production with a TPC value of 4.906 mg GAE/g extract. Pemberian cekaman abiotik pada kultur mikrolga dapat memicu produksi metabolit sekunder yang memiliki beragam bioaktivitas. Senyawa fenolik adalah salah satu metabolit sekunder yang diproduksi oleh Chlorella sp. dan telah dikatagorikan sebagai antioksidan penting yang berperan dalam penangkalan radikal bebas. Penelitian ini menggunakan cahaya ultraviolet (UV)-B (λ 280-320 nm) sebagai cekaman dalam kultur Chlorella sp. Tujuannya adalah untuk menentukan pengaruh radiasi UV-B terhadap produksi biomassa Chlorella sp. dan kadar fenolik total/ total phenolic content (TPC), serta aktivitas antioksidannya. Metode penelitian yang digunakan adalah eksperimental dengan desain rancangan acak lengkap (RAL). Perlakuan yang diujicobakan adalah pemberian radiasi UV-B dengan durasi yang berbeda, yaitu 0 (kontrol), 40, 80, dan 120 menit/hari selama masa kultur. Kultur Chlorella sp. dilakukan menggunakan 12 wadah kaca dengan media kultur berupa air laut (salinitas 35 ppt) dan ditambahkan pupuk Walne (1 mL/L). Selama masa kultur, pemberian cahaya photosynthetically active radiation (PAR) dan aerasi dilakukan selama 24 jam. Biomassa Chlorella sp. dipanen 1 hari setelah tercapai puncak pertumbuhan, kemudian ditentukan berat biomassa dan ekstrak etanolnya, dilanjutkan uji TPC, dan aktivitas antioksidannya. Uji TPC dilakukan menggunakan reagen Folin-Ciocalteu, sedangkan aktivitas antioksidan menggunakan uji inhibisi terhadap 2,2-diphenyl-1-picrylhydrazyl (DPPH). Hasil penelitian menunjukkan bahwa radiasi UV-B tidak berpengaruh signifikan terhadap biomassa Chlorella sp. (p=0,122) dan rendemen ekstrak etanolnya (p=0,194), namun berpengaruh signifikan terhadap TPC (p=0,003) dan persentase inhibisi DPPH (p=0,036). Pemberian radiasi UV-B selama 80 menit/hari selama 10 hari masa kultur Chlorella sp. dapat digunakan untuk meningkatkan produksi fenolik dengan nilai TPC sebesar 4,906 mg GAE/g ekstrak.

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Effects of Ultraviolet-B Radiation in Plant Physiology

Nassour

Ayash

2021

Agriculture (Pol'nohospodárstvo)

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Over the past few decades, anthropogenic activities contributed to the depletion of the ozone layer, which increased the levels of solar ultraviolet-B (UV-B) radiation reaching the Earth`s surface. Generally, UV-B is harmful to all living organisms. It damages the cell`s Deoxyribonucleic acid (DNA), proteins, and lipids, and as a consequence, it affects the bio-membranes negatively. In this review, we summarize the major effects of UV-B in the plant`s main molecules and physiological reactions, in addition to the possible defence mechanisms against UV-B including accumulating UV-B absorbing pigments to alleviate the harmful impact of UV-B.

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The Effect of Ultraviolet Radiation on Chlorophyll in Chlamydomonas reinhardtii

Abstract: The study was conducted during 2017 in the laboratories of the

Cited by 3 publications

References 18 publications

Fabrication of cell plastics composed only of unicellular green alga Chlamydomonas reinhardtii as a raw material

Fabrication of cell plastics composed only of unicellular green alga Chlamydomonas reinhardtii as a raw material

Peningkatan Kadar Fenolik Total dari Chlorella sp. Menggunakan Cekaman Radiasi Ultraviolet-B

Effects of Ultraviolet-B Radiation in Plant Physiology

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