A study of the psychrotrophic bacterial content, determined a t 3-5", of soil, grass and hay showed that these habitats were prolific sources of many different types of psychrotrophs, which sometimes exceeded lO'/g. Untreated farm water supplies had a much lower content, few samples giving colony counts >104/ml. Gram positive or Gram variable, nonsporeforming rods, resembling coryneform bacteria, constituted e relatively high proportion of the psyohrotrophic microflora of soil, Gram negative rods only forming about t of the isolates. In contrast, Pseudomonaa and Acinetobacter spp. and yellow or orange pigmented Gram negative rods were the predominant psychrotrophs in untreated water. Pseudomonao and a taxonomically heterogeneous group of yellow pigmented, Uram negative rods, a few of which resembled Plavobacterium and some Enoinia herbiwla, together with Acinetobacter accounted for nearly 90% of the isolates from grass, but < 60 % of those from hay, which had a more complex psychrotrophio microflora than had grms.[4201