The effect of UV-photofunctionalization on the time-related bioactivity of titanium and chromium–cobalt alloys

Att, Wael; Hori, Norio; Iwasa, Fuminori; Yamada, Masahiro; Ueno, Takeshi; Ogawa, Takahiro

doi:10.1016/j.biomaterials.2009.04.048

Cited by 194 publications

(259 citation statements)

References 26 publications

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“…Numerous studies have shown the ALP activation effect of surface-modified materials 18,23,24) and our results support these conclusions. Furthermore, the observed increase of OCN production in the presence of the nanostructured surfaces is also in agreement with previous findings 25,26) .…”

Section: Discussionsupporting

confidence: 90%

Bioactivity of Titanium Surface Nanostructures Following Chemical Processing and Heat Treatment

Komasa

Taguchi

et al. 2015

J. Hard Tissue Biology.

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Our previous research reports that NaOH treatment leads to the formation of a Ti-O-Na titanate layer on the titanium-6-aluminium-4-vanadium (Ti6Al4V) surface. However, this titanium nanosheets (TNS) hydrogel layer is so brittle that it easily detaches from the implant and can cause many problems, including degradation in the living body due to inhomogeneous composition distribution. The aims of the present study were to investigate combined alkali-treatment of Ti6Al4V alloy and then heated and evaluate the ability of this modified surface to affect osteogenic differentiation of rat bone marrow (RBM) cells, to increase the success rate of titanium implants. We fabricated TNS on titanium alloy surfaces by NaOH treatment prior to heat treatment at 600 °C, and determined RBM cell properties and differentiation potential on the surface in comparison to untreated control surfaces. The nanoscale network structures formed by alkali etching markedly enhanced RBM cell adhesion and osteogenic-related gene expression. Other cell behaviors, such as proliferation, alkaline phosphatase activity, osteocalcin deposition and mineralization, were markedly increased on the TNS-modified Ti6Al4V with heat treatment. Our results suggest that titanium implants modified with nanostructures promote osteogenic differentiation, which may improve the biointegration of these implants into the alveolar bone.

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Section: Discussionsupporting

confidence: 90%

Bioactivity of Titanium Surface Nanostructures Following Chemical Processing and Heat Treatment

Komasa

Taguchi

et al. 2015

J. Hard Tissue Biology.

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“…Although the underlying mechanism remained to be fully elucidated, hydrophilicity was purportedly one of the crucial factors. UV irradiation has been proved to improve the hydrophilicity of titanium surfaces [8][9][10][11][12][13][14][15] . Thus, it might also be a relatively convenient way to improve the bioactivity of titanium surfaces.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that Ca-and P-containing surfaces enhanced both cell response in vitro 5) and osseointegration in vivo 6) . Recently, ultraviolet (UV) irradiation has been reported to improve the hydrophilicity of various modified titanium surfaces [8][9][10][11][12][13][14][15] , to the effect of improving the bioactivity 7,8,10) and cell response [11][12][13][14] of implants both in vitro and in vivo 9,15) . However, the mechanisms that underlie both hydrophilicity and biological response changes were still not fully understood and need further investigation.…”

Section: Introductionmentioning

confidence: 99%

Ultraviolet irradiation enhanced bioactivity and biological response of mesenchymal stem cells on micro-arc oxidized titanium surfaces

Huang

et al. 2015

Dental Materials Journal

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This present study investigated the effect of ultraviolet (UV) irradiation on bioactivity of micro-arc oxidized (MAO) titanium surface in vitro by cell culture medium immersion test and interactions with rat-derived mesenchymal stem cells (MSCs). UV-irradiated MAO surface exhibited no obvious changes in surface roughness, morphology, and phase composition when compared with MAOonly surface. However, in cell culture medium immersion test, markedly more bone-like apatite was formed on UV-modified samples than on MAO sample. Rat bone marrow-and adipose tissue-derived MSCs cultured on UV-modified samples displayed accelerated attachment, significant higher levels of alkaline phosphatase (ALP) activity, and up-regulated osteogenesis-related mRNA expression than MAO sample. XPS results provided direct evidence that the amount of basic hydroxyl groups increased with UV irradiation time, which could be one of the key mechanisms underlying their improved bioactivity.

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“…The as-produced titanium surface is hydrophilic, but with time carbon compounds adhere to the titanium surface, reducing the contact ratio and strength of the titanium/bone contact 23,24) . The carbon compounds on the titanium surface increase sharply over 4 weeks after production, and then gradually over the next 3 months, while absorption of the proteins necessary for cell adhesion and osteogenesis falls notably.…”

Section: Sample Productionmentioning

confidence: 99%

Effect on Osteogenesis of Cleaning Titanium Implants with Ozonated Water

Yoshida

Ando

Sugita

et al. 2016

J. Hard Tissue Biology.

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Carbon compounds accumulate over time on titanium surfaces. This is reported to reduce osseointegration of titanium implants in bone. Ozonated water was found to remove carbon compounds and to have a sufficient cleaning effect. The effects of the ozonated water on the titanium surface were evaluated by cell culture and mechanical tests. In vitro study, titanium disks (diameter 20 mm, thickness 1 mm, Grade 2) were treated with sulfuric acid. Disks in the control group were surface cleaned with distilled water, and disks in the O 3 group were surface cleaned for 10 min with ozonated water. The experimental animals were male Sprague-Dawley (SD) rats. We tested for cell proliferation activity, alkaline phosphatase (ALP) activity, calcification and measured calcium concentrations. In vivo study, titanium implants (diameter 1 mm, length 2 mm, Grade 2) were treated with sulfuric acid. Control group samples were washed for 10 min with distilled water immediately before implantation. O 3 group samples were washed for 10 min with ozonated water. We performed push-in testing using a mechanical testing machine. The femurs were removed at 2 and 4 weeks after implantation. Cell proliferation activity was higher in the O 3 group than in the control group at 24 h after cell seeding. Similarly, ALP activity was higher in the O 3 group than in the control group, indicating higher cell differentiation potential. Cell calcification was higher in the O 3 group than in the control group at all measurement times. Maximum compressive stress was significantly higher in the O 3 group compared with the control group at 2 weeks after implantation (p < 0.05). At 4 weeks after implantation, no difference was found between the groups. Osseointegration time was decreased by cleaning titanium implants with ozonated water.

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The effect of UV-photofunctionalization on the time-related bioactivity of titanium and chromium–cobalt alloys

Cited by 194 publications

References 26 publications

Bioactivity of Titanium Surface Nanostructures Following Chemical Processing and Heat Treatment

Bioactivity of Titanium Surface Nanostructures Following Chemical Processing and Heat Treatment

Ultraviolet irradiation enhanced bioactivity and biological response of mesenchymal stem cells on micro-arc oxidized titanium surfaces

Effect on Osteogenesis of Cleaning Titanium Implants with Ozonated Water

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