The Effect of Vitamin A on Cellular Differentiation and Mucous Glycoprotein Synthesis in Long-Term Rat Tracheal Organ Cultures

Clark, J. R.; Marchok, Ann C.

doi:10.1111/j.1432-0436.1979.tb01026.x

Cited by 32 publications

(13 citation statements)

References 15 publications

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“…Previous studies have shown that growth and differentiation of conjunctival epithelial cells can be modulated by vitamin A (Clark and Marchok, 1979), by matrix components such as collagen gel and matrigel (Tsai and Tseng, 1988), and by inclusion of conjunctival ®broblasts in an organotypic culture (Tsai et al, 1997). When the amniotic membrane (AM) was used to culture rabbit conjunctival epithelial cells (RCE), the authors (Meller and Tseng, 1999) and Cho et al, (1999) noted that non-goblet epithelial differentiation is preferentially promoted despite adding retinoic acid, lifting the culture to the air±medium interface, and co-culturing RCE with rabbit conjunctival ®broblasts (RCF) on the stromal side of AM.…”

Section: Introductionmentioning

confidence: 99%

Expansion of Conjunctival Epithelial Progenitor Cells on Amniotic Membrane

Meller¹,

Dabul²,

Tseng

2002

Experimental Eye Research

136

120

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Section: Introductionmentioning

confidence: 99%

Expansion of Conjunctival Epithelial Progenitor Cells on Amniotic Membrane

Meller¹,

Dabul²,

Tseng

2002

Experimental Eye Research

136

120

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“…Studies with whole animals and in vivo organ culture models showed that during vitamin A deficiency the tracheobronchial epithelium undergoes extensive squamous metaplasia (14)(15)(16)20,23,24). This process is reversible: after treatment with retinoids the normal mucociliary epithelium is restored (12,23).…”

Section: Action Of Retinoidsmentioning

confidence: 99%

“…Many cells are withdrawn from the cell cycle and remain in the resting (Go) phase. During vitamin A deficiency or after mechanical or toxic injury, the balance between proliferation and differentiation is disturbed; the epithelium undergoes hyperplasia followed by squamous metaplasia (14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27).…”

Section: Hyperplasia Squamous Metaplasia and Squamous Cell Carcinomasmentioning

confidence: 99%

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Multistep Process of Squamous Differentiation in Tracheobronchial Epithelial Cells In Vitro: Analogy with Epidermal Differentiation

Jetten¹

1989

Environmental Health Perspectives

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The lung, in particular the bronchial epithelium, is a major site for tumor formation in humans. Environmental factors, such as cigarette smoke, in conjunction with genetic factors are important determinants in this disease. Malignant cells exhibit alterations in their control of proliferation and differentiation. It is believed that the acquisition of defects in the regulation of these processes is important in the process of carcinogenesis. A clear insight into the basic mechanisms of the regulation of proliferation and differentiation is required to understand the molecular mechanisms involved in tumor development and in other pathological conditions. Studies using in vitro cell culture systems of tracheobronchial epithelial cells provide useful models in which to study the regulation of differentiation and proliferation. The clonogenic cells derived from the treacheobronchial epithelium are pluripotent: They have self-renewal capacity and can differentiate along either a normal, mucosecretory, or a squamous cell pathway. Squamous differentiation in tracheobronchial epithelial cells has many morphological, biochemical, and regulatory properties in common with epidermal differentiation. This pathway of differentiation is a multistep process consisting of at least three stages. In the initial stage, cells become committed to terminal cell division. This is followed by the expression of the squamous differentiated phenotype and finally cornification. Various factors, such as several growth factors, retinoids, calcium ions, and phorbol esters, regulate the program of differentiation at different stages. Studies have indicated that the controls of proliferation and differentiation are interrelated. Cell lines established from tracheobronchial epithelial cells expressing SV40 large T-antigen, as well as carcinoma cell lines, exhibit altered responses to growth and differentiation regulatory factors Alterations in the commitment to terminal cell division must be a crucial step in the transition of a normal to a malignant cell.

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“…On the macromolecular level, it was hypothesized (9,24) (7,10,11,76,77) as the result of a decreased capacity to manufacture mucus glycoproteins with specific sugar moieties. Further support for a role for vitamin A in glucoprotein synthesis came from work by Clark and Marchok (76,77 (35,36,(38)(39)(40)78) as an assay for retinoid potency. Tracheas excised from animals deprived of vitamin A and maintained in a serum-free, defined medium for 3 days were suitable for evaluating the effects of retinoids added to the medium.…”

Section: Rna Metabolism and Inhibitors Of Rna Synthesismentioning

confidence: 99%

Biochemical Studies of the Tracheobronchial Epithelium

Mass¹,

Kaufman²

1984

Environmental Health Perspectives

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Tracheobronchial epithelium has been a focus of intense investigation in the field of chemical carcinogenesis. We have reviewed some biochemical investigations that have evolved through linkage with carcinogenesis research. These areas of investigation have included kinetics of carcinogen metabolism, identification of carcinogen metabolites, levels of carcinogen binding to DNA, and analysis of carcinogen-DNA adducts. Such studies appear to have provided a reasonable explanation for the susceptibilities of the respiratory tracts of rats and hamsters to carcinogenesis by benzo(a)pyrene. Coinciding with the attempts to understand the initiation of carcinogenesis in the respiratory tract has also been a major thrust aimed at effecting its prevention both in humans and in animal models for human bronchogenic carcinoma. These studies have concerned the effects of derivatives of vitamin A (retinoids) and their influence on normal cell biology and biochemistry of this tissue. Recent investigations have included the effects of retinoid deficiency on the synthesis of RNA and the identification of RNA species associated with this biological state, and also have included the effects of retinoids on the synthesis of mucus-related glycoproteins.Tracheal organ cultures from retinoid-deficient hamsters have been used successfully to indicate the potency of synthetic retinoids by monitoring the reversal of squamous metaplasia. Techniques applied to this tissue have also served to elucidate features of the metabolism of retinoic acid using high pressure liquid chromatography. In brief, formidable strides have been made in biochemistry specific to this important target tissue, despite the inability to acquire tracheobronchial epithelium in large quantities.

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The Effect of Vitamin A on Cellular Differentiation and Mucous Glycoprotein Synthesis in Long-Term Rat Tracheal Organ Cultures

Cited by 32 publications

References 15 publications

Expansion of Conjunctival Epithelial Progenitor Cells on Amniotic Membrane

Expansion of Conjunctival Epithelial Progenitor Cells on Amniotic Membrane

Multistep Process of Squamous Differentiation in Tracheobronchial Epithelial Cells In Vitro: Analogy with Epidermal Differentiation

Biochemical Studies of the Tracheobronchial Epithelium

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