The effect of vitamin e antioxidant addition in goat milk diluent on the quality of thin-tailed sheep semen

Abstract

Artificial insemination supports the prevention of infectious dispersion in organisms which share the same environment. Meanwhile, spermatozoa usually experience a decrease in quality during processing due to the peroxidation process. This study aims to identify the effect of vitamin E antioxidant addition in goat milk diluent on the quality of thin-tailed sheep semen after storing at 5°C for five days. A total of 4 male thin-tailed sheep were used and the treatments consisted of controls, namely goat milk sperm diluent without vitamin E antioxidant (P0), and others with the addition of vitamin E at a dose of 2 gL-1 diluent (P1); 3 gL-1diluent (P2); and 4 gL-1 (P3). Moreover, the variables observed include individual motility, viability, and intact plasma membrane of spermatozoa after storing at 5°C for five days. The data were analyzed statistically using analysis of variance (ANOVA) with a completely randomized design in one-way. The results showed that the addition of vitamin E antioxidant to goat milk diluent during sperm dilution had a significant effect (P < 0.05) on the motility, viability, and intact plasma membrane. Spermatozoa motility at P0, P1, P2, and P3 were 44.0%, 48.2%, 50.4%, and 53.2%, while the viability was 48.2%, 51.4%, 54.2%, and 56.0%, respectively. Additionally, the intact plasma membranes at P0, P1, P2, and P3 were 41.0%, 46.6%, 48.2%, and 51.4%, respectively. The addition of antioxidant vitamins in goat milk diluent with a concentration of 4 gL-1 produced the best results on sperm quality after cooling. Based on the results, it was concluded that the addition of vitamin E antioxidant to the sperm diluent can improve the quality of thin-tailed sheep chilled semen.

Comparison various level ascorbic acid and lycopene additions in semen diluent enhanced sperm quality of Sapudi ram

“…Incubation enhanced lipid peroxidation because of ROS-induced ATP consumption damage, which hinders sperm motility and membrane integrity (21). Intriguing findings showed that administration of ascorbic acid at dosages >3% caused a reduction in pre-freezing semen quality and worsened post-thawing sperm quality, albeit still providing better data than the control on the entire parameter.…”

“…Ejaculate (100 µL) was transferred to a clean, warm, dry glass slide, observed under a microscope, and scored on a scale from 0 (no motility) to 100 (excellent motility). Spermatozoa viability and abnormalities were defined using a fixed smear stained with eosin, and the percentages of live and dead sperm were estimated (21).…”