The effect of wheat-rye substitution on chromosome elimination: An analysis of univalents’ behavior in wheat meiosis with dimonosomy and tetramonosomy

Silkova, O. G.; Kabanenko, Yu. N.; Loginova, D. V.

doi:10.1134/s102279541402015x

Cited by 5 publications

(5 citation statements)

References 17 publications

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“…These double monosomic addition lines can contribute to go deeper into the knowledge of how chromosomes recognize and associate in pairs in the wheat background in the presence of the Ph1 locus. In addition, most of the works carrying alien chromosomes in the wheat background are focused in meiosis metaphase I or later stages (Molnár-Láng et al, 2000 ; Silkova et al, 2014 ). The analysis of chromosome pairing focused only in metaphase I can result in an underestimation of homoeologous associations that might occur earlier in meiosis, as chromosomes might remain mostly as univalents due to the lack of homoeologous recombination.…”

Section: Discussionmentioning

confidence: 99%

“…The knowledge about univalent behavior in meiosis is necessary for the directed development of wheat lines carrying alien introgressions since univalent are subjected of incorrect division and segregation. Thus, abnormities in meiosis result in various modifications and/or in the loss of a transferred chromosome (Silkova et al, 2014 ). Univalents in meiosis have a tendency to misdivide (break) across their centromeres producing telocentric.…”

Section: Discussionmentioning

confidence: 99%

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Homoeologous Chromosomes From Two Hordeum Species Can Recognize and Associate During Meiosis in Wheat in the Presence of the Ph1 Locus

et al. 2018

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Understanding the system of a basic eukaryotic cellular mechanism like meiosis is of fundamental importance in plant biology. Moreover, it is also of great strategic interest in plant breeding since unzipping the mechanism of chromosome specificity/pairing during meiosis will allow its manipulation to introduce genetic variability from related species into a crop. The success of meiosis in a polyploid like wheat strongly depends on regular pairing of homologous (identical) chromosomes and recombination, processes mainly controlled by the Ph1 locus. This means that pairing and recombination of related chromosomes rarely occur in the presence of this locus, making difficult wheat breeding trough the incorporation of genetic variability from related species. In this work, we show that wild and cultivated barley chromosomes associate in the wheat background even in the presence of the Ph1 locus. We have developed double monosomic wheat lines carrying two chromosomes from two barley species for the same and different homoeology chromosome group, respectively. Genetic in situ hybridization revealed that homoeologous Hordeum chromosomes recognize each other and pair during early meiosis in wheat. However, crossing over does not occur at any time and they remained always as univalents during meiosis metaphase I. Our results suggest that the Ph1 locus does not prevent chromosome recognition and pairing but crossing over between homoeologous. The role of subtelomeres in chromosome recognition is also discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Homoeologous Chromosomes From Two Hordeum Species Can Recognize and Associate During Meiosis in Wheat in the Presence of the Ph1 Locus

et al. 2018

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“…Most of the studies performed during meiosis in wheat plants carrying alien chromosomes are focused on meiosis metaphase I or later stages [35,46,47]. Previously, we studied interspecific chromosome associations in early meiosis stages (pachytene) and we managed to track simultaneously extra wild and cultivated barley chromosomes (for the same and for different homoeology groups) in the wheat background using in situ hybridization [21].…”

Section: Discussionmentioning

confidence: 99%

Wild and Cultivated Homoeologous Barley Chromosomes Can Associate and Recombine in Wheat in the Absence of the Ph1 Locus

Calderón

Prieto

2021

Agronomy

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Bread wheat is an allohexaploid that behaves as a diploid during meiosis, the cell division process to produce the gametes occurring in organisms with sexual reproduction. Knowledge of the mechanisms implicated in meiosis can contribute to facilitating the transfer of desirable traits from related species into a crop like wheat in the framework of breeding. It is particularly interesting to shed light on the mechanisms controlling correct pairing between homologous (equivalent) chromosomes and recombination, even more in polyploid species. The Ph1 (Pairing homoeologous 1) locus is implicated in recombination. In this work, we aimed to study whether homoeologous (equivalent chromosomes from different genomes) Hordeum chilense (wild barley) and H. vulgare (cultivated barley) chromosomes can associate and recombine during meiosis in the wheat background in the absence of the Ph1 locus. For this, we have developed H. chilense and H. vulgare double monosomic addition lines for the same and for different homoeology group in wheat in the ph1b mutant background. Using genomic in situ hybridization, we visualized the two (wild and cultivated) barley chromosomes during meiosis and we studied the processes of recognition, association, and recombination between homoeologous chromosomes in the absence of the Ph1 locus. Our results showed that the Ph1 locus does not prevent homoeologous chromosome pairing but it can regulate recombination.

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“…Studies of the meiotic behaviour of 2D and 2R in dimonosomic lines of wheat showed no pairing between them (Silkova et al 2014). The only T2R.2DL translocations found thus far have resulted from ruptures in the centromeric region, which were followed by centric fusion; thus, these events did not involve small chromosome segments (Krasilova et al 2012).…”

Section: Introductionmentioning

confidence: 91%

The effect of 2D(2R) substitution on the agronomical traits of winter triticale in early generations of two connected crosses

Bazhenov

Divashuk

Kroupin

et al. 2015

Cereal Research Communications

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The association between genomic constitution and agronomic traits was studied in F 2 plants and F 3:4 families of two crosses between a winter hexaploid triticale line with a 2D(2R) chromosome substitution and two hexaploid triticale cultivars carrying the complete rye genome (BBAARR). The analyses revealed that 2D(2R) substitution reduces plant height and spikelet number per spike, increases the 1,000-kernel weight, does not reduce grain shrivelling, and promotes early heading and anthesis. 2D(2R) substitution lines exhibit deeper postharvest seed dormancy, which provides resistance to preharvest sprouting. However, 2D(2R) substitution lines are not recommended for winter hexaploid triticale cultivar development purposes due to their reduced grain productivity.

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The effect of wheat-rye substitution on chromosome elimination: An analysis of univalents’ behavior in wheat meiosis with dimonosomy and tetramonosomy

Cited by 5 publications

References 17 publications

Homoeologous Chromosomes From Two Hordeum Species Can Recognize and Associate During Meiosis in Wheat in the Presence of the Ph1 Locus

Homoeologous Chromosomes From Two Hordeum Species Can Recognize and Associate During Meiosis in Wheat in the Presence of the Ph1 Locus

Wild and Cultivated Homoeologous Barley Chromosomes Can Associate and Recombine in Wheat in the Absence of the Ph1 Locus

The effect of 2D(2R) substitution on the agronomical traits of winter triticale in early generations of two connected crosses

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