The effect of yeast extract and methyl jasmonate on rosmarinic acid accumulation in Coleus blumei hairy roots

Bauer, Nataša; Kiseljak, Divor; Jelaska, Sibila

doi:10.1007/s10535-009-0118-8

Cited by 46 publications

(21 citation statements)

References 45 publications

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“…There have been several reports on RA production in roots transformed by A. rhizogenes at higher levels than in roots of field-grown plants. For example, 50 mg RA per gram of dry weight was found in hairy roots of Coleus blumei, while the roots of parent plant produced RA at the level of 17 mg g -1 dry weight (Bauer et al 2009). In hairy roots cultures of Hyssopus officinalis, the production of RA increased about 60 % compared to normal roots of the plant species (Kochan et al 1999).…”

Section: Resultsmentioning

confidence: 99%

Hairy roots of Dracocephalum moldavica: rosmarinic acid content and antioxidant potential

et al. 2013

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Hairy roots of Dracocephalum moldavica L. were induced using Agrobacterium rhizogenes strain A4. Transformed roots were obtained from shoot explants with low transformation frequency of up to 3 %. The effects of different liquid media: Murashige and Skoog (MS), Gamborg et al. (B5) and Woody Plant (WP) with full-and half-strength ( MS, B5, WP), on biomass accumulation and rosmarinic acid (RA) content were investigated. The hairy roots were cultured in photoperiod (16 h light/8 h dark) and darkness. Biomass of D. moldavica hairy roots was the highest (7.23 g flask -1 of fresh weight and 0.89 g flask -1 of dry weight) in the cultures grown in WP medium under periodic light. Ultra performance liquid chromatography analysis revealed the highest RA content (78 mg g -1 dry wt) in roots cultured in B5 medium under photoperiod conditions. It was about tenfold higher compared to roots of field-grown mother plants. Antioxidant activities and total phenolic contents of methanolic extracts of D. moldavica hairy roots cultured in B5 and WP media under photoperiod and darkness and roots of field grown plants were compared. All extracts were investigated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging and phosphomolybdenum reduction assays. Total phenolic contents were estimated by the FolinCiocalteu method. The methanolic extract of D. moldavica hairy roots grown in B5 medium under photoperiod possessed the strongest effects on reducing Mo and DPPH radical scavenging. The activities were significantly higher (p B 0.05) than those of methanolic extract of roots of intact plants grown in the field. The most active methanolic extract of hairy roots was characterized by the highest level of rosmarinic acid and total content of phenolic compounds.

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Section: Resultsmentioning

confidence: 99%

Hairy roots of Dracocephalum moldavica: rosmarinic acid content and antioxidant potential

et al. 2013

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“…Elicitors as compounds or treatments have been proved to be an effective method to increase secondary metabolite production. A number of elicitors such as methyl jasmonate (MJ) [14][15][16][17] and salicylic acid (SA) [14,18] have been used successfully for enhanced production of secondary metabolites in hairy root cultures of many plant species. In addition, fungal elicitors are significant in triggering phytoalexin production in cultured cells and have been used for enhanced production of secondary metabolites in some medicinal plants [19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Enhanced production of valerenic acid in hairy root culture of Valeriana officinalis by elicitation

Torkamani

Jafari²,

Abbaspour

et al. 2014

Open Life Sciences

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Valerenic acid (VA) is a pharmacologically-active sesquiterpene found in valerian (Valeriana officinalis L., Valerianaceae) roots and rhizomes. The plant produces only small amounts of this metabolite naturally. So, induction of hairy roots as well as elicitation can be useful to increase its commercial production. In this study, Wild-type strain ‘A13’ of Agrobacterium rhizogenes was used to induce hairy roots in valerian. The influence of three different elicitors including Fusarium graminearum extract (FE), methyl jasmonate (MJ) and salicylic acid (SA) on VA production in the selected hairy root line ‘LeVa-C4’ was also investigated. The 23-day-old cultures were treated with different concentrations of the elicitors at exposure time of 3 and 7 days. FE (1%) and MJ (100 µM L−1) highly promoted VA production at 7 days after elicitation, to a level of 12.31- and 6-fold higher than that of non-elicited controls, respectively, and FE did not exert any negative effects on biomass yield of hairy root. SA did not significantly increase the production of VA. This is the first time study to assess the elicitation of hairy root cultures to promote VA biosynthesis in valerian and the resulting experiments demonstrated that F. graminearum extract and MJ were indeed a potent inducer of VA biosynthesis.

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“…In our previous investigation we have shown the stimulating eff ect of methyl jasmonate and yeast extract on RA production in hairy roots (22). Here, the application of ABA strongly enhanced RA production and did not aff ect growth, which makes ABA one of the strongest inducers, as well as the most promising agent for elicitation of RA synthesis in C. blumei hairy roots.…”

Section: The Eff Ect Of Plant Growth Regulators On Growth and Rosmarimentioning

confidence: 74%

“…Normal roots were regenerated on young leaf explants cultured on MS medium supplemented with 1 mg/L of NAA. Tumour induction was performed on young leaf explants cultured in hormone-free MS medium following transformation with Agrobacterium tumefaciens A281 and B6S3 strains while hairy roots were induced with Agrobacterium rhizogenes A4 strain (21,22). Tumours and hairy roots were maintained on hormone--free MS medium.…”

Section: Plant Materials and Cultivation Conditionsmentioning

confidence: 99%

Potential of Different Coleus blumei Tissues for Rosmarinic Acid Production

Bauer

Vuković

Likić

et al. 2015

Food Technol. Biotechnol.

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SummaryRosmarinic acid is one of the main active components of Coleus blumei and is known to have numerous health benefi ts. The pharmacological signifi cance of rosmarinic acid and its production through in vitro culture has been the subject of numerous studies. Here, the ability of diff erent tissues to accumulate rosmarinic acid and sustainability in production over long cultivation have been tested. Calli, tumours, normal roots and hairy roots were established routinely by application of plant growth regulators or by transformation with agrobacteria. The diff erences among the established tumour lines were highly heterogeneous. Hairy root lines showed the highest mean growth rate and consistency in rosmarinic acid production. Although some tumour lines produced more rosmarinic acid than the hairy root lines, over a long cultivation period their productivity was unstable and decreased. Further, the eff ects of plant growth regulators on growth and rosmarinic acid accumulation were tested. 2,4-Dichlorophenoxyacetic acid signifi cantly reduced tumour growth and rosmarinic acid production. 1-Naphthaleneacetic acid strongly stimulated hairy root growth whilst abscisic acid strongly enhanced rosmarinic acid production. Hairy roots cultured in an airli bioreactor exhibited the highest potential for mass production of rosmarinic acid.

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The effect of yeast extract and methyl jasmonate on rosmarinic acid accumulation in Coleus blumei hairy roots

Cited by 46 publications

References 45 publications

Hairy roots of Dracocephalum moldavica: rosmarinic acid content and antioxidant potential

Hairy roots of Dracocephalum moldavica: rosmarinic acid content and antioxidant potential

Enhanced production of valerenic acid in hairy root culture of Valeriana officinalis by elicitation

Potential of Different Coleus blumei Tissues for Rosmarinic Acid Production

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