The effect of zinc on the growth and physiological and biochemical parameters in seedlings of Eruca sativa (L.) (Rocket)

Abstract

Eruca sativa seedlings were treated with different Zn concentrations (0, 250, 500, 1,000, 2,000 lg g -1 dried growth medium) under controlled conditions. The seedlings were harvested 20 days after Zn treatment. Physiological parameters, such as root and shoot length, fresh and dry weight, were measured and Zn content of roots and shoots was determined. Furthermore, various biochemical parameters were studied on E. sativa leaves: enzymatic antioxidants, such as superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (POD), ascorbate peroxidase (APX), and non-enzymatic antioxidants, such as ascorbate, non-protein thiols. Malondialdehyde, which is an index of lipid peroxidation, was assayed. Zn treatment did not have any significant effect on fresh and dry weights, whereas 500 lg g -1 Zn increased root growth significantly (p \ 0.05). Zn accumulated in roots 2-8 times more than it did in leaves. Lipid peroxidation increased in proportion with the increase in Zn. Although a decrease in SOD and CAT activities at increased Zn was found, a significant increase in APX and POD was observed at 500 and 1,000 lg g -1 Zn, respectively. In addition, an increase in the amounts of non-protein thiols and total AsA (Ascorbate) was observed with the increase in Zn.

The effects of cadmium on the biochemical and physiological parameters of Eruca sativa

“…In this study, SOD activity decreased in all Cd concentrations when compared with that of the control but the decrease in 450 µg • g -1 was significant statistically. Similarly, it was reported in the other researches that SOD activity decreased in toxic concentrations of some heavy metals [13,29]. Cd is a redox inactive metal and it has no direct effect on the SOD activity.…”

“…The analysis showed that lipid peroxidation, the most distinctive indication of oxidative stress, increased due to the increase in cadmium concentration. A similar responses were observed in plants treated with Cd and the other heavy metal [29,32,35]. The enzymes, such as SOD, CAT, PODx are considered to play an important role in the cellular defense mechanisms against ROS caused by heavy metal toxicity.…”

“…Italy and Turkey. Eruca sativa was determined to be tolerant against some heavy metals [29]. Based on this information, the aim of the research, was to determine effects of Cd on biochemical and physiological parameters of the leaves and the roots of E. sativa, and tolerance level to Cd or the accumulation ratio of parts of the plant.…”

Zinc stress induces physiological, ultra-structural and biochemical changes in mandarin orange (Citrus reticulata Blanco) seedlings

“…Leaf and root tissues of the mandarin plants, subjected to Zn-deficiency and -excess, generated AsA in lower quantity (Fig. 5a) probably due to higher activity of APX, which along with AsA are the components of Halliwel-Asada pathway (Ozdener and Aydin 2010). In our study, starch contents dwindled in the Zn-deficient and Zn-toxic plants (Fig.…”

Nitric Oxide Is Associated with Long-Term Zinc Tolerance in Solanum nigrum

“…Many studies have indicated that PCD plays a role in the developmental plasticity of plant architecture (Duan et al, 2010). Excess Zninduced growth inhibition and root death have been found in various plant species (Lingua et al, 2008;Ozdener and Aydin, 2010). However, whether Zninduced root death occurs through PCD and its underlying mechanisms are poorly understood.…”