The effect on immunocytes of anodic oxide titanium after hydrothermal treatment

Abstract

All dental root implants come in contact with the oral epithelium, and many complex factors are found to arise in this region. In order to perform a successful dental root implantation, it is necessary to clarify the interaction of the dental root implant material with the host defense mechanisms involved in the specific and nonspecific immune responses to many antigens in oral bacteria and their components. Recently, focusing on developing the dental root implant, the Nikon Corporation improved the surface characteristics of pure titanium even further by developing a hydroxyapatite (HA) layer formed on an anodic titanium oxide film containing Ca and P via hydrothermal treatment (SA treatment). However, since little is known about the effect of SA-treated pure titanium (HA/Ti) on the defense mechanisms of the oral membrane epithelium, we investigated (1) the in vitro proliferation of murine splenic B lymphocytes on the surface of HA/Ti in the presence of three lipopolysaccharide (LPS) concentrations and (2) interleukin-1alpha (IL-1alpha) production by the reaction of human peripheral blood mononuclear cells (PBM cells) on the surface of HA/Ti under the same concentrations. After culture, murine splenic lymphocytes were measured by uptake of 3H-thymidine, and cytokine release (IL-1alpha) from PBM cells was measured by ELISA. Results showed that HA/Ti had hardly any effect on the LPS-induced proliferation of B lymphocytes and IL-1alpha production. In vitro investigations of the effects of HA/Ti on the LPS-induced proliferation of murine splenic B lymphocytes and IL-1alpha from PBM cells might be a useful way of elucidating the defense mechanism between implants and the oral epithelium.

Modification of titanium oxide layer by calcium and phosphorus

“…The oxide layer enriched in this way is characterized by increased biotolerance. The biotolerance of titanium anodized in a solution containing calcium and phosphorus can be additionally improved by means of a hydrothermal treatment [25][26][27]. Moreover, the application of plasma electrolytic oxidation in solutions containing calcium and phosphorus leads to an increase of corrosion resistance of titanium in physiological saline solutions [18,28,29].…”

Anodic oxidation and hydrothermal treatment of commercially pure titanium surfaces increases expression of bone morphogenetic protein‐2 in the adherent macrophage cell line J774A.1

“…Studies have confirmed that a HA/cpTi surface promotes early production and mineralization of bone matrix,14, 15 and early bone formation. A HA/cpTi surface is compatible with bone tissue,16 and has no adverse effect on immune cells 17. Furthermore, the thin HA layer formed on HA/cpTi increases in stability during the process of bone matrix mineralization 18.…”

Association of the composite IL‐1 genotype with peri‐implantitis: a systematic review

“… No genotype testing: Perala et al (1992), Kao et al (1995), Mengel et al (1996), Lamster et al (1996), Panagakos et al (1996), Salcetti et al (1997), Cuenin et al (1997), Curtis et al (1997), Aboyoussef et al (1998), Schierano et al (2003), Pietruski et al (2001), Hultin et al (2002), Murata et al (2002), Schultze‐Mosgau et al (2006), Konttinen et al (2006), Liskmann et al (2006), Machtei et al (2006), Lachmann et al (2007b). In vitro studies: Koka & Reinhardt (1997), Wakabayashi et al (1997), Takebe et al (1998), Reyes‐Botella et al (2002), Ataoglu et al (2002), Spyrou et al (2002), Schierano et al (2003). Experimental studies: Katsuki et al (1998), Nor et al (2001), Schierano et al (2005). No data on oral implants: Becker & Rosenbaum (2000), Rhoton‐Vlasak et al (2005). Review article: Greenstein & Hart (2002a, 2002b), Nares (2003), Hwang & Wang (2007). Cross‐sectional design: Campos et al (2005a), Lachmann et al (2007a). …”

“…In vitro studies: Koka & Reinhardt (1997), Wakabayashi et al (1997), Takebe et al (1998), Reyes‐Botella et al (2002), Ataoglu et al (2002), Spyrou et al (2002), Schierano et al (2003).…”