The effective and safe method for preventing and treating bacteria-induced dental diseases by herbal plants and a recombinant peptide

Abstract

Background This study was conducted aimed at evaluating the antibacterial property of the recombinant peptide of bacteriocin entrocin P (EnP), the essential oil of Cuminum cyminum, and the extract of Ferulago angulata on some oral pathogens. Besides, the cytotoxicity of EnP was evaluated. Material and Methods The antimicrobial property was tested on streptococcus mutans (ATCC 35668), streptococcus salivarius (ATCC 9222), streptococcus oralis (ATCC 35037), and Enterococcus faecalis (ATCC 29212), using the microbroth dilution method. The 0.2% Chlorhexidin (CHX) mouthwash was used as the control group. Besides, the cytotoxicity analysis was done on gingival fibroblasts by the MTT colorimetric method. The data were reported using descriptive methods, and analyzed by one-way ANOVA, and Tukey’s HSD test. Results The strongest bacteriostatic and bactericidal effects of C. cyminum and F. angulata were observed for S.mutans and S. oralis , respectively (with the MIC and MBC value being 62.5 μg/mL). The antibacterial properties of EnP were comparable to those of CHX, being several times stronger than medicinal plants (1-14 μg/mL). Based on the cytotoxicity evaluation, there was no statistically significant difference observed between the cytotoxicity of the control group and that of Enp for three evaluations, except after 72 hours when the cell viability at the concentration of 3.75 µg/ml was significantly lower than that of the control group ( P =0.05). However, no concentration of EnP was observed to be over 50% of the growth inhibition (IC50) of the fibroblasts for the three evaluations. Conclusions EnP could be utilized in dental materials as a natural and safe antimicrobial agent against oral streptococci and E. faecalis, being as effective as CHX mouthwash. Key words: Antimicrobial peptide, Bacteriocin Entrocin P, Chlorhexidine, Cuminum cyminum, Enterococcus faecalis, Ferulago angulata.

Thymus richardii subsp. nitidus (Guss.) Jalas Essential Oil: An Ally against Oral Pathogens and Mouth Health

“…Dental researchers are developing and testing new therapeutic substances that are low-or non-toxic to prevent or eradicate dental plaque-related disorders. For this reason, three oral pathogenic strains were chosen as Gram-positive (S. mutans, S. oralis, and S. aureus) [67] and three oral and/or opportunistic pathogenic strains as Gram-negative bacteria (P. aeruginosa, S. Typhimurium, and E. coli) [68]. As can be seen in Figure 3, there is a proportionality between the increase in EO concentration and the decrease in bacterial survival.…”

Extraction of Cuminum cyminum and Foeniculum vulgare Essential Oils and Their Antibacterial and Antibiofilm Activity against Clinically Isolated Porphyromonas gingivalis and Prevotella intermedia: An In Vitro Study

“…The use of natural products such as essential oils (EOs) as antimicrobial agents has increased in medicine and dentistry over the last few decades because of their safety and widespread acceptance by the population worldwide. Herbal products are effective against infections, carcinomas, and cardiovascular and neurological disorders [12].…”