The paper presents the results of research on the justification of parameters of transport operation of three-section main (train) UzTE16M3 freight diesel locomotives on the high-speed railway section Marokand- Kattakurgan of JSC “Uzbekistan Temir Yullari” when moving freight trains without stops and with stops at intermediate stations, passing points, and separate points. The specified results were obtained by methods of locomotive traction theory in the form of tabular data, graphical dependences, and regression equations for determining logistical kinematic parameters of freight train traffic and the main energy efficiency indicators of the investigated diesel locomotives in a given section. The research results obtained by the authors can be used by drivers - instructors in thermal engineering and specialists of linear enterprises of JSC “Uzbekistan Temir Yullari” locomotive complex, whose professional and production activity is connected with the issues of saving of natural diesel fuel consumption by diesel locomotives on high-speed sections of the railroads.