The effectiveness of a 595 nm pulsed-dye-laser in the treatment of surgical scars following a knee arthroplasty

Pongcharoen, Padcha; Pongcharoen, Boonchana; Disphanurat, Wareeporn

doi:10.1080/14764172.2019.1661488

Cited by 12 publications

(19 citation statements)

References 21 publications

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“…Untreated HSs can also bring about some physiological and psychological disturbance from cosmetic dis gurement and even economic burden for the victims [2]. Clinically, lasers of diverse parameters are recognized as effective and safe treatment options for HSs, especially fractional CO2 laser and 595-nm PDL [5][6][7][8][9][10][11][12][13][14]. The primary mechanisms of the two laser systems may be involved in some inter-cell factors and pathways in skin resurfacing, such as BMP-7 and Fas expression [29-33, 45, 46].…”

Section: Discussionmentioning

confidence: 99%

“…Fractional CO2 laser and 595-nm PDL in treating hypertrophic scars build on the principle of selective photothermolysis, and their e cacies have been clinically proved [9][10][11][12][13][14][35][36][37][38][39][40][41][42][43][44][45][46]. In current study, we attempted combined use of the two laser modalities in rabbit models, with BMP-7 and Fas expression detected in the HS tissues.…”

Section: Discussionmentioning

confidence: 99%

“…HSs are characterized by signs of hard and red nodules, and symptoms of pain and itch, and treatment of them primarily rely on corticosteroid injection, laser therapy and surgery [4][5][6][7][8]. Fractional CO2 laser(FCO2L) and 595-nm pulsed dye laser (PDL) are selective phototherapeutics, and have been validated to be effective in therapy of HS [9][10][11][12][13][14], yet their underlying mechanisms remain unclear, and need validation through measurement of bone morphogenetic protein-7(BMP-7) and Fas gene, the two important proteins that affect the prognosis of this condition on molecular study basis. BMP-7, a TGF-β superfamily, has been shown to effectively inhibit the brosis process through regulating cell proliferation, differentiation and apoptosis in various organs and tissuesin series of studies [15][16][17][18][19][20][21][22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

“…Both FCO2L and 595-nm PDL have been validated to be conventional treatment options for HSs with clinical safety and e cacy [9][10][11][12][13][14]. FCO2L for HS is based on fractional and micro-holes with almost identical density and depth, and suppresses abnormal and irregulated brosis and collagen, thus reducing scar formation at the HSs [40][41][42][43][44][45][46].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, 595-nm PDL can create deeper penetration than 585-nm PDL, and destroy extra hemoglobin and blood vessels at the HS and other skin conditions [35][36][37][38]. The effectiveness of 595-nm PDL for HSs has been evaluated and documented in some studies [9][10][11][12][35][36][37][38][39].…”

Section: Introductionmentioning

confidence: 99%

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Fractional CO2 laser combined with 595-nm PDL inhibiting hypertrophic scars via measuring BMP-7 and Fas expression in rabbit models

Zhang¹,

Zhou²,

Xia³

et al. 2020

Preprint

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Background and ObjectivesAlthough the treatment of hypertrophic scar (HS) remain challenging, fractional CO2 laser (FCO2L) and 595-nm pulsed-dye laser (PDL) have proved clinical efficacy. Meanwhile, BMP-7 and Fas proteins are demonstrated to promote wound healing and inhibit scar formation, yet few reports on the effect of the two proteins on hypertrophic scarring are available, and their molecular mechanisms remain unclear. In current study, we attempted to observe the effect of combined use of FCO2L with 595-nm PDL in HS animal models through determining the expression of BMP-7 and Fas in scar inhibition.Materials and MethodsTwenty New Zealand white rabbits were randomized to control group, FCO2L group, PDL group and combined treatment group. Four HS samples were developed at each ear of individual rabbit. FCO2L was respectively applied to simple FCO2L and combined treatment group, and simple 595-nm PDL and combined treatment group. Totally, 3 sessions of treatments were carried out once every 14 days. Then, the changes of fibroblasts and collagens in HSs and expression of BMP-7 and Fas proteins in the scar tissues were determined via histological and immunohistochemical studies, ELISA, CCK8 test, RT-PCR and Western blot assay.ResultsHSs were flattened and shrunk after treatment, especially in rabbits treated by FCO2L plus 595-nm PDL group, in which obviously decreased abnormal fibroblast and collagen were noted. The deference was significant compared to other groups(P < 0.001). Moreover, expression of BMP-7 and Fas was both increased in the combined treatment group compared to single FCO2L or 595-nm PDL therapy group (P < 0.001) .ConclusionsFCO2L combined with 595-nm PDL can improve HSs in rabbit models by inhibiting excessive fibroblast growth and collagen deposition. This may be associated with increased BMP-7 and Fas expression in the scar tissues. And our findings may pioneer a new therapeutic strategy for alternative treatment of HSs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Fractional CO2 laser combined with 595-nm PDL inhibiting hypertrophic scars via measuring BMP-7 and Fas expression in rabbit models

Zhang¹,

Zhou²,

Xia³

et al. 2020

Preprint

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Verifying the outcomes of artesunate plus 595‐nm PDL in hypertrophic scars via determining BMP‐7 and Fas level in model rabbits

et al. 2022

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Background and Objectives: Single-use of artesunate (ART) or 595-nm pulsed-dye laser (PDL) has proven clinical efficacy in the treatment of hypertrophic scars (HSs), yet little research has been done on the combined use of ART and PDL. Bone morphogenetic protein-7 (BMP-7) and Fas are recognized to be two important proteins in reducing scar formation. This study was designed to observe the effect of ART combined with 595-nm PDL in the treatment of HS in rabbit models, and investigate the effect of such protocol on the expression of BMP-7 and Fas in rabbit models. Study Design/Materials and Methods: Twenty-four New Zealand white rabbits were randomly divided into the control group, ART group, PDL group, and combined treatment (ART + PDL) group. ART was respectively applied to the ART group and combined treatment group. Treatment was once every 2-week for a total of three sessions for both groups. Animals in the PDL group were simply treated with 595-nm PDL. Then, hematoxylin & eosin and Van Gieson straining, immunohistochemical study, enzyme-linked immunosorbent assay (ELISA), Cell counting kit-8 test, western blot assay, and real-time polymerase chain reaction (RT-PCR) were carried out to observe the development of HS samples and expression of BMP-7 and Fas proteins in the sample tissues. Results: After treatment, the scar samples grew lower and flatter, which was particularly evident in the combined treatment group, with notably inhibited fibroblast and collagen compared to other groups (p < 0.001). Western blot assay and RT-PCR demonstrated that the expression of BMP-7 was most increased in scar samples treated by ART + PDL. BMP-7 level was correspondingly and notably upregulated in treatment groups, especially in the ART + PDL group. In addition, relevant expression of Fas was also higher after treatment, especially in the ART + PDL group compared to either ART or 595-nm PDL group. The difference was significant among groups (p < 0.001). Conclusions: Combined use of ART and 595-nm PDL can inhibit HSs in rabbit models via inhibiting extra fibroblast and collagens. The potential mechanism may be involved in enhanced BMP-7 and Fas expression. Our observations may create an alternative therapeutic strategy for HSs in the clinic.

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595-nm pulsed dye laser combined with fractional CO2 laser reduces hypertrophic scar through down-regulating TGFβ1 and PCNA

et al. 2021

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The effectiveness of a 595 nm pulsed-dye-laser in the treatment of surgical scars following a knee arthroplasty

Cited by 12 publications

References 21 publications

Fractional CO2 laser combined with 595-nm PDL inhibiting hypertrophic scars via measuring BMP-7 and Fas expression in rabbit models

Fractional CO2 laser combined with 595-nm PDL inhibiting hypertrophic scars via measuring BMP-7 and Fas expression in rabbit models

Verifying the outcomes of artesunate plus 595‐nm PDL in hypertrophic scars via determining BMP‐7 and Fas level in model rabbits

595-nm pulsed dye laser combined with fractional CO2 laser reduces hypertrophic scar through down-regulating TGFβ1 and PCNA

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