The effects of 2,4‐dinitrophenol and <scp>d</scp>‐glucose concentration on the development, sex ratio, and interferon‐tau (IFNT) production of bovine blastocysts

Green, Mark P.; Harvey, Alexandra J; Spate, Lee D.; Kimura, Koji; Thompson, Jeremy G.; Roberts, R. Michael

doi:10.1002/mrd.22590

Cited by 17 publications

(12 citation statements)

References 74 publications

(122 reference statements)

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“…This increase in glucose concentrations was associated with delayed embryo development as measured by conceptus elongation on d 14. Although this finding disagrees with our original hypothesis, it is consistent with in vitro results reported by Green et al (2016), who altered glucose metabolism through either glucose supplementation or uncoupling oxidative phosphorylation with 2,4-dinitrophenol (DNP) between d 5 and 8 after in vitro fertilization. In that study, greater glucose concentrations (4.0 vs. 0 mM) during embryo culture substantially reduced bovine blastocyst development on d 8 (Green et al, 2016).…”

Section: Embryo Growth and Developmentsupporting

confidence: 59%

“…Although this finding disagrees with our original hypothesis, it is consistent with in vitro results reported by Green et al (2016), who altered glucose metabolism through either glucose supplementation or uncoupling oxidative phosphorylation with 2,4-dinitrophenol (DNP) between d 5 and 8 after in vitro fertilization. In that study, greater glucose concentrations (4.0 vs. 0 mM) during embryo culture substantially reduced bovine blastocyst development on d 8 (Green et al, 2016). Earlier work by Rieger et al (1995) suggested that increased rates of glucose metabolism induced by conditioned culture medium were unfavorable for subsequent embryo development.…”

Section: Embryo Growth and Developmentsupporting

confidence: 59%

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The effect of exogenous glucose infusion on early embryonic development in lactating dairy cows

Leane

Herlihy

Curran

et al. 2018

Journal of Dairy Science

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The objective of this study was to examine the effect of intravenous infusion of glucose on early embryonic development in lactating dairy cows. Nonpregnant, lactating dairy cows (n = 12) were enrolled in the study (276 ± 17 d in milk). On d 7 after a synchronized estrus, cows were randomly assigned to receive an intravenous infusion of either 750 g/d of exogenous glucose (GLUC; 78 mL/h of 40% glucose wt/vol) or saline (CTRL; 78 mL/h of 0.9% saline solution). The infusion period lasted 7 d and cows were confined to metabolism stalls for the duration of the study. Coincident with the commencement of the infusion on d 7 after estrus, 15 in vitro-produced grade 1 blastocysts were transferred into the uterine horn ipsilateral to the corpus luteum. All animals were slaughtered on d 14 to recover conceptuses, uterine fluid, and endometrial tissue. Glucose infusion increased circulating glucose concentrations (4.70 ± 0.12 vs. 4.15 ± 0.12 mmol/L) but did not affect milk production or dry matter intake. Circulating β-hydroxybutyrate concentrations were decreased (0.51 ± 0.01 vs. 0.70 ± 0.01 mmol/L for GLUC vs. CTRL, respectively) but plasma fatty acids, progesterone, and insulin concentrations were unaffected by treatment. Treatment did not affect either uterine lumen fluid glucose concentration or the mRNA abundance of specific glucose transporters in the endometrium. Mean conceptus length, width, and area on d 14 were reduced in the GLUC treatment compared with the CTRL treatment. A greater proportion of embryos in the CTRL group had elongated to all length cutoff measurements between 11 and 20 mm (measured in 1-mm increments) compared with the GLUC treatment. In conclusion, infusion of glucose into lactating dairy cows from d 7 to d 14 post-estrus during the critical period of conceptus elongation had an adverse impact on early embryonic development.

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Section: Embryo Growth and Developmentsupporting

confidence: 59%

Section: Embryo Growth and Developmentsupporting

confidence: 59%

The effect of exogenous glucose infusion on early embryonic development in lactating dairy cows

Leane

Herlihy

Curran

et al. 2018

Journal of Dairy Science

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“…On day 7 of development, in vitro -produced female bovine blastocysts show a higher incidence of cell apoptosis than male blastocysts (Ghys et al, 2016), with the earliest detection of this effect on day 6 (Oliveira et al, 2016). Dimorphism in terms of metabolic strategy is also a distinct; this is demonstrated by the differential uptake of amino acids between male and female embryos (Sturmey et al, 2010), and was shown in a study by Green et al (2016) in which alteration of glucose availability in culture medium induced a stronger skew in sex ratio of bovine blastocysts.…”

Section: Introductionmentioning

confidence: 99%

Sexual Dimorphism of miRNAs Secreted by Bovine In vitro-produced Embryos

2017

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Sexual dimorphism of bovine blastocysts has previously been observed through differences in development, cell death, metabolism, telomere length, DNA methylation, and transcriptomics. However, dimorphism in the secretion of miRNAs to culture media has not yet been evaluated. The objectives of this study were to determine if sex-specific blastocyst miRNA secretion occurs and to further investigate the role these miRNAs may have in the interaction between a blastocyst and the maternal environment. In vitro embryo culture was performed and media from male and female blastocysts was collected into sex-specific pools. Profiling of 68 miRNAs revealed a total of eight miRNAs that were differentially expressed between female and male-conditioned media. Validation by qPCR confirmed higher expression of miR-22 (P < 0.05), miR-122 (P < 0.05), and miR-320a (P < 0.05) in female media for three additional biological replicates. To examine the potential roles of secreted miRNAs to the media in communication with the maternal environment, miR-22, miR-122, and miR-320a were each supplemented to four replicates of primary bovine endometrial epithelial cell culture. Uptake of miR-122 (P < 0.05) and miR-320a (P < 0.05) was detected, and a trend of uptake was detected for miR-22 (P > 0.05). Further, expression of the progesterone receptor transcript, a predicted target of all three miRNAs, was found to be upregulated in the cells following supplementation of miR-122 (P < 0.05) and miR-320a (P < 0.05), and a trend upregulation of the transcript was observed following miR-22 (P > 0.05) supplementation. This work demonstrates that male and female conceptuses are able to differentially secrete miRNAs at the blastocyst stage and that these miRNAs have the ability to induce a transcriptomic response when applied to maternal cells. This knowledge builds on the known dimorphic differences in conceptuses at the blastocyst stage and demonstrates a role for blastocyst-secreted miRNAs in cell–cell communication.

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“…All reagents were sourced from Sigma-Aldrich unless otherwise stated. The methods for oocyte collection, maturation, fertilisation and in vitro culture to the 8-cell stage have been previously described 43 44 . Briefly, bovine cumulus-oocyte-complexes (COC) were collected from antral follicles from abattoir-derived ovaries in HEPES-buffered tissue culture medium-199 (TCM 199) supplemented with 10% fetal calf serum (FCS; Life Technologies) and 4 mg/mL fatty acid-free bovine serum albumin (FAF-BSA; MP BioMedical).…”

Section: Methodsmentioning

confidence: 99%

Bisphenol A affects early bovine embryo development and metabolism that is negated by an oestrogen receptor inhibitor

Choi

Harvey

Green

2016

Sci Rep

Self Cite

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Increasing evidence supports an association between exposure to endocrine disruptors, such as the xenoestrogen bisphenol A (BPA), a commonly used plasticiser, and the developmental programming of offspring health. To date however animal studies to investigate a direct causal have mainly focussed on supra-environmental BPA concentrations, without investigating the effect on the early embryo. In this study we investigated the effect of acute BPA exposure (days 3.5 to 7.5 post-fertilisation) at environmentally relevant concentrations (1 and 10 ng/mL) on in vitro bovine embryo development, quality and metabolism. We then examined whether culturing embryos in the presence of the oestrogen receptor inhibitor fulvestrant could negate effects of BPA and 17β-oestradiol (E2). Exposure to BPA or E2 (10 ng/mL) decreased blastocyst rate and the percentage of transferrable quality embryos, without affecting cell number, lineage allocation or metabolic gene expression compared to untreated embryos. Notably, blastocysts exposed to BPA and E2 (10 ng/mL) displayed an increase in glucose consumption. The presence of fulvestrant however negated the adverse developmental and metabolic effects, suggesting BPA elicits its effects via oestrogen-mediated pathways. This study demonstrates that even acute exposure to an environmentally relevant BPA concentration can affect early embryo development and metabolism. These may have long-term health consequences on an individual.

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The effects of 2,4‐dinitrophenol and d‐glucose concentration on the development, sex ratio, and interferon‐tau (IFNT) production of bovine blastocysts

Cited by 17 publications

References 74 publications

The effect of exogenous glucose infusion on early embryonic development in lactating dairy cows

The effect of exogenous glucose infusion on early embryonic development in lactating dairy cows

Sexual Dimorphism of miRNAs Secreted by Bovine In vitro-produced Embryos

Bisphenol A affects early bovine embryo development and metabolism that is negated by an oestrogen receptor inhibitor

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