The effects of −80 °C short-term storage on the mechanical response of tricuspid valve leaflets

Salinas, Samuel D.; Clark, Margaret; Amini, Rouzbeh

doi:10.1016/j.jbiomech.2019.109462

Cited by 19 publications

(8 citation statements)

References 35 publications

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“…We also observed that the strain at high stress values in the radial direction differed significantly in the control group post-treatment. Though not expected, this significant difference could be a result of tissue creep following the first set of loading protocols (as observed previously [27,28]) or because of the degradation of the tissue. Lastly, the responses of the tissue following elastin digestion were based on an ECM structure comprised of collagen that has experienced a sudden loss of undulation.…”

Section: Plos Onementioning

confidence: 51%

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The role of elastin on the mechanical properties of the anterior leaflet in porcine tricuspid valves

et al. 2022

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Elastin is present in the extracellular matrix (ECM) of connective tissues, and its mechanical properties are well documented. In Marfan syndrome, however, the inability to properly code for the protein fibrillin-1 prematurely leads to the degradation and loss of elastin fiber integrity in the ECM. In this study, the role of elastin in the ECM of the anterior leaflet of the tricuspid valve was investigated by examining the biomechanical behavior of porcine leaflets before and after the application of the enzyme elastase. Five loading protocols were applied to the leaflet specimens in two groups (elastase-treated and control samples). The mechanical response following elastase application yielded a significantly stiffer material in both the radial and circumferential directions. At a physiological level of stress (85 kPa), the elastase group had an average strain of 26.21% and 6.32% in the radial and circumferential directions, respectively, at baseline prior to elastase application. Following elastase treatment, the average strain was 5.28% and 0.97% in the radial and circumferential directions, respectively. No statistically significant change was found in the control group following sham treatment with phosphate-buffered saline (PBS). Two-photon microscopy images confirmed that after the removal of elastin, the collagen fibers displayed a loss of undulation. With a significant reduction in radial compliance, the ability to withstand physiological loads may be compromised. As such, an extracellular matrix that is structurally deficient in elastin may hinder normal tricuspid valve function.

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Section: Plos Onementioning

confidence: 51%

“…The radial direction, which is orthogonal to the circumferential direction, was by default aligned to the remaining axis on the phantom, shown in Fig 1B . Further details of this sample preparation procedure have been previously been described at length. [25][26][27][28].…”

Section: Sample Preparationmentioning

confidence: 99%

The role of elastin on the mechanical properties of the anterior leaflet in porcine tricuspid valves

et al. 2022

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“…There is no consensus on the literature as to whether freezing affects the mechanical properties of biological soft tissues. Indeed, some studies showed that there is not any significant difference in the stiffness between fresh and frozen tissues (43)(44)(45)(46), while other studies found an increase (47) or a decrease (48) in the stiffness of frozen tissue compared to fresh tissue. The elastic modulus of breast tissue obtained our study were in agreement with the values obtained on fresh tissue (7,10), suggesting that freezing did not significantly alter the mechanical properties of these tumour tissues.…”

Section: Discussionmentioning

confidence: 99%

Mechanical properties of breast, kidney, and thyroid tumours measured by AFM: Relationship with tissue structure

et al. 2022

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“…There is no consensus on the literature as to whether freezing affects the mechanical properties of biological soft tissues. Indeed, some studies showed that there is not any significant difference in the stiffness between fresh and frozen tissues (43)(44)(45)(46), while other studies found an increase (47) or a decrease (48) in the stiffness of frozen tissue compared to fresh tissue. The elastic modulus of breast tissue obtained in our study were in agreement with the values obtained on fresh tissue (7,10), suggesting that freezing did not significantly alter the mechanical properties of these tumour tissues.…”

Section: Discussionmentioning

confidence: 99%

Mechanical properties of breast, kidney, and thyroid tumours measured by AFM: relationship with tissue structure

Levillain

Confavreux

Decaussin‐Petrucci

et al. 2022

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The mechanical properties of the extracellular matrix are essential for regulating cancer cell behaviour, but how they change depending on tumour type remains unclear. The aim of the current study was to determine how the mechanical properties of tumours that frequently metastasize to bones were affected depending on histological type. Human breast, kidney, and thyroid specimens containing tumour and normal tissue were collected during surgery. The elastic modulus and elastic fraction of each sample were characterised using atomic force microscopy and compared with histopathological markers. We observed that tumour mechanical properties were differentially affected depending on organ and histological type. Indeed, clear cell renal carcinoma and poorly differentiated thyroid carcinoma displayed a decrease in the elastic modulus compared to their normal counterpart, while breast tumours, papillary renal carcinoma and fibrotic thyroid tumours displayed an increase in the elastic modulus. Elastic fraction decreased only for thyroid tumour tissue, indicating an increase in the viscosity. These findings suggest a unique mechanical profile associated with each subtype of cancer. Therefore, viscosity could be a discriminator between tumour and normal thyroid tissue, while elasticity could be a discriminator between the subtypes of breast, kidney and thyroid cancers.

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The effects of −80 °C short-term storage on the mechanical response of tricuspid valve leaflets

Cited by 19 publications

References 35 publications

The role of elastin on the mechanical properties of the anterior leaflet in porcine tricuspid valves

The role of elastin on the mechanical properties of the anterior leaflet in porcine tricuspid valves

Mechanical properties of breast, kidney, and thyroid tumours measured by AFM: Relationship with tissue structure

Mechanical properties of breast, kidney, and thyroid tumours measured by AFM: relationship with tissue structure

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The effects of −80 °C short-term storage on the mechanical response of tricuspid valve leaflets

Cited by 19 publications

References 35 publications

The role of elastin on the mechanical properties of the anterior leaflet in porcine tricuspid valves

The role of elastin on the mechanical properties of the anterior leaflet in porcine tricuspid valves

Mechanical properties of breast, kidney, and thyroid tumours measured by AFM: Relationship with tissue structure

Mechanical properties of breast, kidney, and thyroid tumours measured by AFM: relationship with tissue structure

Contact Info

Product

Resources

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The effects of −80 °C short-term storage on the mechanical response of tricuspid valve leaflets