The Effects of a Gastric Inhibitory Substance on Gastric Secretion in the Pylorus Ligated Rat¹

Abstract

An extract was prepared from juice secreted by vagally denervated isolated pouches of the gastric antrum in the dog, by dialyzing against water and lyophilization. This gastric inhibitory substance (GIS) was assayed in pylorus ligated rats. Three groups of rats served as controls. One group received no injections, while the two other were injected with saline (1 ml/100 g rat) intravenously (i.v.) or intraperitoneally (i.p.). Two different types of gastric secretion were studied: (a) basal or spontaneous gastric output, and (b) gastric acid response to exogenous gastrin. GIS lowered significantly the basal or spontaneous output of acid, but did not alter the basal output of pepsin significantly. When GIS was injected into animals stimulated by gastrin, there was a highly significant lower output of both acid and pepsin.

Studies on some Quaternary Compounds with an Inhibitory Effect on the Gastric Secretion of Rats

“…However, substances of totally different types have also been reported to have effect. SEMB (1966) found a preparation gained from the antrum of dogs to have a considerable inhibitory effect on Shay rats-a mechanism of action which presumably can be left out of account in the present study. BASS et al.…”

The Effect of Gastric Juice Extracts on Histamine as a Gastric Secretory Stimulant

“…The doses of the extracts from antrum or fundus were also of the same order of magnitude as those shown previously to give inhibition upon i.v. injection (Semb 1966a(Semb , 1969. I n earlier studies, it was shown that inhibition of gastric acid secretion took place about 3/4-1 hr after the i.v.…”

“…injection of extracts from gastric juice. Extracts prepared from human anacid gastric juice and juice of isolated antral pouches in the dog are the richest source of this inhibitor (Brunschwig et al 1939, 1940, Hood, Code and Grindlay 1953, Semb 1966a . However, the mode of action of this "gastric inhibitory substance" (GIS) is not clear.…”

Secretion of Hydrochloric Acid in Ghosh-Lai Rat Preparation following Stimulation with Histamine and Pentagastrin and Inhibition by Gastrone and Atropine

“…Gastrone is the endogenous inhibitor of gastric acid secre tion (9) described by Brunschwig et al (4) in anacid human gastric juice and determined by means of bioassays on dogs with Pavlov pouches. Its inhibitory activity on HC1 output has been thereafter studied by Blackburn et al (2) and Code et al (5) on dogs with Heidenhain pouches and by other authors on rats with pyloric ligation (6,12,13) or gastric fistula (1). Gastrone B is the impor tant inhibitor of hydrochloric acid secretion contained in 'fraction 7' of Fiasse et al (6) separated from the anacid gastric juice on Scphadex G-100 column.…”