The effects of abiotic stresses on the NADP-dependent malic enzyme in the leaves of the hexaploid wheat

Fu, Z. Y.; Zhang, Z. B.; Liu, Z. H.; Hu, Xiaojun; Xu, Pei

doi:10.1007/s10535-011-0030-x

Cited by 19 publications

(10 citation statements)

References 34 publications

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“…Previous studies reported that the accumulation of enolase was upregulated in different varieties under drought stress and other abiotic stresses [ 53 , 55 – 57 ]. The activity of the NADP-dependent malic enzyme (NADP-ME) was typically enhanced under various stresses, including those caused by PEG, SA, cold, darkness and NaCl, and RT-PCR showed that the transcript accumulation of NADP-ME in the leaves was distinctly affected by various stresses [ 58 ]. The upregulated accumulation of cytosolic aconitase plays a role in mediating oxidative stress and regulating cell death, which might enhance the tolerance of wheat to drought stress [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of Leaf Proteins Differentially Accumulated between Wheat Cultivars Distinct in Their Levels of Drought Tolerance

Cheng

Dong

et al. 2015

PLoS ONE

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The drought-tolerant ‘Ningchun 47’ (NC47) and drought-sensitive ‘Chinese Spring’ (CS) wheat (Triticum aestivum L.) cultivars were treated with different PEG6000 concentrations at the three-leaf stage. An analysis on the physiological and proteomic changes of wheat seedling in response to drought stress was performed. In total, 146 differentially accumulated protein (DAP) spots were separated and recognised using two-dimensional gel electrophoresis. In total, 101 DAP spots representing 77 unique proteins were identified by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. These proteins were allocated to 10 groups according to putative functions, which were mainly involved in carbon metabolism (23.4%), photosynthesis/respiration (22.1%) and stress/defence/detoxification (18.2%). Some drought stress-related proteins in NC47, such as enolase, 6-phosphogluconate dehydrogenase, Oxygen-evolving enhancer protein 2, fibrillin-like protein, 2-Cys peroxiredoxin BAS1 and 70-kDa heat shock protein, were more upregulated than those in CS. Multivariate principal components analysis revealed obvious differences between the control and treatments in both NC47 and CS, while cluster analysis showed that the DAPs displayed five and six accumulation patterns in NC47 and CS, respectively. Protein–protein interaction network analysis showed that some key DAPs, such as 2-Cys peroxiredoxin BAS1, RuBisCO large subunit-binding protein, 50S ribosomal protein L1, 6-phosphogluconate dehydrogenase, glyceraldehyde 3-phosphate dehydrogenase isoenzyme and 70-kDa heat shock protein, with upregulated accumulation in NC47, had complex interactions with other proteins related to amino acid metabolism, carbon metabolism, energy pathway, signal transduction, stress/defence/detoxification, protein folding and nucleotide metabolism. These proteins could play important roles in drought-stress tolerance and contribute to the relatively stronger drought tolerance of NC47.

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Section: Discussionmentioning

confidence: 99%

Identification of Leaf Proteins Differentially Accumulated between Wheat Cultivars Distinct in Their Levels of Drought Tolerance

Cheng

Dong

et al. 2015

PLoS ONE

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“…UV-B radiation [40] . Increased NADP-ME activity in transgenic Arabidopsis by osmotic stress induced a greater salt tolerance [41] , wheat NADP-ME also responded to various abiotic stresses [42] . Heat shock proteins are well known to be induced by all kinds of stress conditions and are efficient to protect cells against these stresses.…”

Section: Discussionmentioning

confidence: 98%

Differences in Grain Ultrastructure, Phytochemical and Proteomic Profiles between the Two Contrasting Grain Cd-Accumulation Barley Genotypes

et al. 2013

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To reveal grain physio-chemical and proteomic differences between two barley genotypes, Zhenong8 and W6nk2 of high- and low- grain-Cd-accumulation, grain profiles of ultrastructure, amino acid and proteins were compared. Results showed that W6nk2 possesses significantly lower protein content, with hordein depicting the greatest genotypic difference, compared with Zhenong8, and lower amino acid contents with especially lower proportion of Glu, Tyr, Phe and Pro. Both scanning and transmission electron microscopy observation declared that the size of A-type starch molecule in W6nk2 was considerably larger than that of Zhenong8. Grains of Zhenong8 exhibited more protein-rich deposits around starch granules, with some A-type granules having surface pits. Seventeen proteins were identified in grains, using 2-DE coupled with mass spectrometry, with higher expression in Zhenong8 than that in W6nk2; including z-type serpin, serpin-Z7 and alpha-amylase/trypsin inhibitor CM, carbohydrate metabolism, protein synthesis and signal transduction related proteins. Twelve proteins were less expressed in Zhenong8 than that in W6nk2; including barley trypsin inhibitor chloroform/methanol-soluble protein (BTI-CMe2.1, BTI-CMe2.2), trypsin inhibitor, dehydroascorbate reductase (DHAR), pericentrin, dynein heavy chain and some antiviral related proteins. The data extend our understanding of mechanisms underlying Cd accumulation/tolerance and provides possible utilization of elite genetic resources in developing low-grain-Cd barley cultivars.

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“…Salicylic acid plays an important role in modulating plant responses to abiotic and biotic stresses (Senaratna et al 2000, Ding et al 2002, Tasgin et al 2006, Wen et al 2008, Fu et al 2011, Saruhan et al 2012, Mutlu et al 2013b.…”

Section: Discussionmentioning

confidence: 99%

Identification of alternatively spliced MsRan transcripts involved in low temperature response in Musa spp.

Zhang¹,

Fang²,

Zhang³

2017

Biologia plant.

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Ran is involved in response to external stimuli. In this study, six MsRan gene cDNA sequences were isolated from wild banana (Musa spp. AB group) from Sanming City, China. Sequence analysis reveals that MsRan3A, MsRan3A-1a, and MsRan3C contained Ran protein domains including a GTP hydrolysis domain, a RanGAP-binding domain, and an acidic tail, whereas two G boxes (G4 and G5) were absent in MsRan3A-6a. The physicochemical property of MsRan3A, MsRan3A-1a, MsRan3A-6a, and MsRan3C appeared to differ significantly. Real time quantitative PCR (qPCR) analysis indicates that MsRan3A-1, MsRan3A-5, MsRan3A-6, MsRan3A-6a, and MsRan3C-1 were expressed in roots, leaves, peduncles, bracts, flowers, peels, and pulp of the wild banana. MsRan3A-1a was expressed at extremely low levels in these tissues and was undetectable by qPCR. The MsRan genes were found to be involved in responses to a low temperature stress but with different response patterns. Furthermore, salicylic acid significantly enhanced MsRan gene expressions suggesting the involvement of these genes in salicylic acid signal transduction.

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The effects of abiotic stresses on the NADP-dependent malic enzyme in the leaves of the hexaploid wheat

Cited by 19 publications

References 34 publications

Identification of Leaf Proteins Differentially Accumulated between Wheat Cultivars Distinct in Their Levels of Drought Tolerance

Identification of Leaf Proteins Differentially Accumulated between Wheat Cultivars Distinct in Their Levels of Drought Tolerance

Differences in Grain Ultrastructure, Phytochemical and Proteomic Profiles between the Two Contrasting Grain Cd-Accumulation Barley Genotypes

Identification of alternatively spliced MsRan transcripts involved in low temperature response in Musa spp.

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