The Effects of Adenoviral Transfection of the Keratinocyte Growth Factor Gene on Epidermal Stem Cells: an In Vitro Study

Li, Xinping; Liang, Ling; Zhao, Pengwei; Uchida, Kenzo; Baba, H.; Huang, Huiyan; Bai, Wenfang; Bai, Liming; Zhang, Mingsheng

doi:10.1007/s10059-013-0093-y

Cited by 6 publications

(6 citation statements)

References 38 publications

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“…Monoculture models of the epithelium offer a useful platform to study the re-epithelialization process. The cultures consist of keratinocytes (preferably HaCaT cells, an immortalized human skin cell line [147]), primary keratinocytes, or epidermal stem cells [139,[148][149][150][151]. The cell line is grown in a monolayer in vitro and is subjected to a number of assays relevant to the wound healing process [139].…”

Section: In Vitro Wound Healing Modelsmentioning

confidence: 99%

“…The cell line is grown in a monolayer in vitro and is subjected to a number of assays relevant to the wound healing process [139]. Many studies have used the presence of cytokines [138,[152][153][154][155], growth factors [148,[156][157][158][159][160], or the expression of several markers of the ECM [161,162] as standards for the measurement of the wound healing processes [139]. Co-culture cell models more closely resemble the in vivo epithelial structure and consist of cells that are normally in communication during the wound healing process.…”

Section: In Vitro Wound Healing Modelsmentioning

confidence: 99%

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Current Methods for the Discovery of New Active Ingredients from Natural Products for Cosmeceutical Applications

Espinosa-Leal

García‐Lara

2019

Planta Med

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Cosmeceuticals are designed to serve a dual purpose: to provide desired esthetical effects and to treat dermatological conditions. Natural products derived from plants and marine organisms are a novel source of potential cosmeceutical active ingredients for incorporation into new formulations due to consumer demands. Contrary to common perceptions, most regulatory agencies do not view cosmeceuticals as being a separate category from cosmetics; thus, these products are not regulated accordingly, thereby forcing the consumer to rely on the self-regulatory policies of the cosmetics industry. Cosmeceuticals are advertised as having capabilities that include anti-aging, anti-acne, solar-protective, wound healing, and skin whitening. Such traits normally comprise several biological activities. In order to ensure the safety and efficacy of these products, active ingredients employed in the formulations must undergo a series of tests. In this review, in vitro (enzymatic and cellular) and in vivo tests employed to evaluate the potential of new cosmeceutical active ingredients are discussed, and new trends that are being explored by the cosmeceutical industry are described.

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Section: In Vitro Wound Healing Modelsmentioning

confidence: 99%

Section: In Vitro Wound Healing Modelsmentioning

confidence: 99%

Current Methods for the Discovery of New Active Ingredients from Natural Products for Cosmeceutical Applications

Espinosa-Leal

García‐Lara

2019

Planta Med

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“…Western blot analysis for KGF expression was carried out using the tissue proteins. Briefly, tissue proteins were obtained from the extracts of the epidermis of mice, and Western blot was performed as described in Li et al 13 Antibody reaction was done with antibodies against KGF (Boster, Wuhan, China; dilution 1: 200), GAPDH (Santa Cruz Biotechnology, Santa Cruz, CA; dilution 1: 2,000).…”

Section: Western Blot Analysismentioning

confidence: 99%

“…11 Keratinocyte growth factor (KGF), known as fibroblast growth factor 7, is a paracrine growth factor for keratinocytes, which could inhibit terminal differentiation of cultured keratinocytes, 12 and stimulate ESCs to growth and cell division. 13 Guo et al. 14 has shown that KGF was required for hair development.…”

Section: Introductionmentioning

confidence: 99%

Exposure to 50 Hz electromagnetic fields enhances hair follicle regrowth in C57BL/6 mice

Wang

Bai

et al. 2019

Exp Biol Med (Maywood)

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Many studies have suggested that electromagnetic field activity affects the cellular activity of many types of cells involved in forming hair follicles. However, the bio-effects of electromagnetic fields on hair follicle growth have not been fully elucidated. This present study was designed to determine whether 50 Hz electromagnetic fields increased hair follicle regrowth. In this experiment, C57BL/6 mice were used to present the model of depilation-induced hair follicle cycling, and then those mice were divided at random into the control group and the electromagnetic field group. After electromagnetic field (50 Hz, 5 mT) exposure for 16 days, the skin specimens of the mice were harvested to assess for hair regrowth, and epidermal stem cells proliferation was evaluated by immunofluorescence staining. The expression and location of keratinocyte growth factors were also tested. Our results showed that, compared to the control, the hair club formed faster on the 3rd day, and most of the hair shafts erupted earlier from the pore in the epidermis on the 9th day after depilation, and the hairs length was significantly longer on the 16th day within the electromagnetic field group. After electromagnetic field treatment, there were more Ki67+ cells in the outer root sheath and hair bulb where it co-localized with K15+ cells compared to the control. Keratinocyte growth factors were expressed in the inner root sheath in both groups, and the electromagnetic field group showed more expression of keratinocyte growth factors. Our data suggested that the hair-growth-promoting effect of the 50 Hz electromagnetic field was observed in depilation-induced hair follicles cycling, which was associated with 50 Hz electromagnetic field enhancing K15+ stem cells proliferation and increased keratinocyte growth factor expression. Impact statement In this study, our experiments confirmed that 50 Hz EMF affected hair follicle regrowth, and 50 Hz EMF enhanced K15+ stem cells proliferation in the hair bulb and follicular outer root sheath of hair follicles. Those results indicated that 50 Hz EMF may be beneficial for functional healing of hair loss.

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“…Signaling pathways such as Wnt [ 10 ], TGF-β [ 11 , 12 ], MAPK [ 13 ], Shh [ 14 ], and Notch [ 15 ] are extensively involved in different components of hair follicle morphogenesis and its periodic variation. In addition, some cytokines also play an important role in hair follicle development and hair growth processes, including KGF [ 16 ], IGF [ 17 ], VEGF [ 18 ], EGF [ 19 ], and thymosin β4 [ 20 ], which promote the growth of hair follicles, and FGF-5 [ 21 , 22 ], which inhibits hair growth. MicroRNAs (miRNAs) are endogenously expressed, highly conserved small RNAs with 21–25 nucleotides that are derived from one arm of their precursor with a stem-loop structure that is expressed in specific cells and tissues [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

An Integrated Analysis of MicroRNA and mRNA Expression Profiles to Identify RNA Expression Signatures in Lambskin Hair Follicles in Hu Sheep

Sun

Yin

et al. 2016

PLoS ONE

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Wave patterns in lambskin hair follicles are an important factor determining the quality of sheep’s wool. Hair follicles in lambskin from Hu sheep, a breed unique to China, have 3 types of waves, designated as large, medium, and small. The quality of wool from small wave follicles is excellent, while the quality of large waves is considered poor. Because no molecular and biological studies on hair follicles of these sheep have been conducted to date, the molecular mechanisms underlying the formation of different wave patterns is currently unknown. The aim of this article was to screen the candidate microRNAs (miRNA) and genes for the development of hair follicles in Hu sheep. Two-day-old Hu lambs were selected from full-sib individuals that showed large, medium, and small waves. Integrated analysis of microRNA and mRNA expression profiles employed high-throughout sequencing technology. Approximately 13, 24, and 18 differentially expressed miRNAs were found between small and large waves, small and medium waves, and medium and large waves, respectively. A total of 54, 190, and 81 differentially expressed genes were found between small and large waves, small and medium waves, and medium and large waves, respectively, by RNA sequencing (RNA-seq) analysis. Differentially expressed genes were classified using gene ontology and pathway analyses. They were found to be mainly involved in cell differentiation, proliferation, apoptosis, growth, immune response, and ion transport, and were associated with MAPK and the Notch signaling pathway. Reverse transcription-polymerase chain reaction (RT-PCR) analyses of differentially-expressed miRNA and genes were consistent with sequencing results. Integrated analysis of miRNA and mRNA expression indicated that, compared to small waves, large waves included 4 downregulated miRNAs that had regulatory effects on 8 upregulated genes and 3 upregulated miRNAs, which in turn influenced 13 downregulated genes. Compared to small waves, medium waves included 13 downregulated miRNAs that had regulatory effects on 64 upregulated genes and 4 upregulated miRNAs, which in turn had regulatory effects on 22 downregulated genes. Compared to medium waves, large waves consisted of 13 upregulated miRNAs that had regulatory effects on 48 downregulated genes. These differentially expressed miRNAs and genes may play a significant role in forming different patterns, and provide evidence for the molecular mechanisms underlying the formation of hair follicles of varying patterns.

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The Effects of Adenoviral Transfection of the Keratinocyte Growth Factor Gene on Epidermal Stem Cells: an In Vitro Study

Cited by 6 publications

References 38 publications

Current Methods for the Discovery of New Active Ingredients from Natural Products for Cosmeceutical Applications

Current Methods for the Discovery of New Active Ingredients from Natural Products for Cosmeceutical Applications

Exposure to 50 Hz electromagnetic fields enhances hair follicle regrowth in C57BL/6 mice

An Integrated Analysis of MicroRNA and mRNA Expression Profiles to Identify RNA Expression Signatures in Lambskin Hair Follicles in Hu Sheep

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