The effects of amlodipine and platelet rich plasma on bone healing in rats

Abstract

AimThe aim of this study was to evaluate the effects of calcium channel blocker (CCB) amlodipine (AML), platelet rich plasma (PRP), and a mixture of both materials on bone healing.Materials and methodsFifty-six male Wistar rats were randomly divided into four groups: group A, tibia defect model with no treatment; group B, tibia defect model treated with AML, 0.04 mg daily by oral gavage; group C, tibia defect model treated with local PRP; group D, tibia defect model treated with local PRP and AML, 0.04 mg daily by oral gavage.ResultsAt day 21, bone healing was significantly better in groups C and D compared to group A (P<0.05), but comparisons showed no statistically significant difference in group B (P>0.05). At day 30, groups B and C showed no statistically significant difference (P>0.05) compared to group A, but bone healing in group D was significantly better than in group A (P<0.05). Statistically, AML did not affect alkaline phosphatase (ALP) activity at 21 and 30 days (P>0.05), but PRP and AML + PRP increased ALP activity statistically (P<0.05).ConclusionIt can be concluded that AML had neither a positive nor a negative effect on bone healing, but when used in combination with PRP, it may be beneficial.

Histological and immunohistochemical study of the potential healing effects of glucosamine and platelets rich plasma on experimentally induced oral mucositis in adult male rats

“…Furthermore a significant decrease of expression caspase reaction in the cytoplasm of buccal epithelial cells, while a significant increase of expression PCNA immunostaining of these cells as compared with group II. These results were an identity with previous researchers [12,17,[32][33][34], who stated that PRP promotes healing in a different form of lesions because it has many important bioactive proteins hastens the endothelial, mesenchymal, epithelial and epidermal Intralesional injection of PRP was proceeded as it offers a selective spreading in the targeted parts of the wound that require more aid in the healing process and therefore a reduction in the required volume of PRP [35]. Both recent experimental and clinical studies [11,17] have revealed that intralesional injection of PRP in wound healing and muscle injuries lead to better regeneration, increasing neovascularization, reducing fibrosis and scarring.…”

“…PRP promotes bone, muscle, tendon, cartilage and skin growth and minimizes both pain and inflammation [11][12][13]. PRP is an autologous output of blood and it formed of a high platelet concentration in a small quantity of plasma.…”

“…PRP is an autologous output of blood and it formed of a high platelet concentration in a small quantity of plasma. It has great advantage on clinical proposal as it is an cheap product, easy to obtain, and there is no risk of rejection or immune reaction and also had an antimicrobial action [12,14].…”

Pentoxifylline and electromagnetic field improved bone fracture healing in rats

“…Swift bone fracture healing after traumas and surgical procedures is crucial in order to regain normal functioning of the bone and its surrounding structures. 32 There have been an increasing number of studies on this subject. 25 , 32 Although the outcome of various clinical and surgical procedures in bone healing is affected by several factors, which may be patient-, defect-, or surgery-related variables, 32 blood flow and circulation play a significant role in the process of bone remodeling and the reparative regeneration of bone tissue.…”

“… 32 There have been an increasing number of studies on this subject. 25 , 32 Although the outcome of various clinical and surgical procedures in bone healing is affected by several factors, which may be patient-, defect-, or surgery-related variables, 32 blood flow and circulation play a significant role in the process of bone remodeling and the reparative regeneration of bone tissue. 33 , 34 After the third day of the fracture in our current study, there was a significant rise in local blood flow, which is associated with vascular proliferation in the formation of fracture callus.…”