The effects of anions on sodium transport

Cuthbert, A. W.; Painter, Elisabeth; Prince, William

doi:10.1111/j.1476-5381.1969.tb08307.x

Cited by 16 publications

(8 citation statements)

References 12 publications

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“…Stimulatory and inhibitory effects of anionic replacements of C1 on active Na transport have been noted in frog skin (Ferriera, 1968;Cuthbert, Painter & Prince, 1969;Fischbarg, Zadunaisky & DeFisch, 1967) and toad urinary bladder (Singer & Civan, 1971). Singer and Civan (1971) reported that the relative abilities of anions (in the mucosal solution) to stimulate active Na transport across toad urinary bladder paralleled the "lyotropic series" (or "Hofmeister series") suggesting that the effect was due to interaction of these anions with relatively weak cationic sites.…”

Section: Possible Mechanism(s) Of Anion Actionmentioning

confidence: 94%

Effect of anions on amiloride-sensitive, active sodium transport across rabbit colon,in Vitro

1977

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Replacement of Cl in the solutions bathing partial mucosal strips of rabbit descending colon with sulfate, isethionate, hydroxypropane-sulfonate and, to a lesser degree, ethanesulfonate stimulates active Na absorption (JNanet) when the baso-lateral pump mechanism is not saturated. These effects are rapid in onset and are readily reversible. Our findings indicate that these stimulatory anions decrease the resistance of the amiloridesensitive Na entry step at the mucosal membrane (RmNa). However, when the active Na pump mechanism at the baso-lateral membrane is saturated these stimulatory anions do not decrease the resistance of the Na entry process. These findings suggest the presence of a negative feedback between the activity of the pump mechanism and the resistance of the Na entry step which may be mediated by the size of the intracellular Na transport pool. In other words, it seems that when the baso-lateral pump is operating at its maximal rate the resistance to Na entry across the mucosal membrane through the amiloride-sensitive pathway is at a minimum and cannot be further decreased.

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Section: Possible Mechanism(s) Of Anion Actionmentioning

confidence: 94%

Effect of anions on amiloride-sensitive, active sodium transport across rabbit colon,in Vitro

1977

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“…HALL AND OTHERS short-circuit current. Since an increase in chloride permeability may largely contribute to this (Cuthbert & Painter, 1968a, b;Cuthbert, Painter & Prince, 1969) skins were mounted in sulphate Ringer. In these circumstances, theophylline (2.5 x 10-M) caused a significant increase in shortcircuit current (Table 3).…”

Section: Effect Of Arachidonic Acid On Short-circuit Currentmentioning

confidence: 99%

Endogenous prostaglandins, adenosine 3':5'‐monophosphate and sodium transport across isolated frog skin.

Hall¹,

O'Donoghue²,

O'Regan³

et al. 1976

The Journal of Physiology

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1. Sodium transport across isolated frog skin, as measured by the short-circuit current, was decreased by acetylsalicylic acid, mefenamic acid, paracetamol and phenylbutazone. Indomethacin (6 x 106 M) had a biphasic effect on the short-circuit current: a transient increase followed by a sustained decrease. 2. The release of prostaglandin-like material from the skin was reduced by acetylsalicylic acid and indomethacin. Paracetamol caused a significant reduction in the short-circuit current response of the skin to low doses of arachidonic acid, but the response to the highest dose tested was not significantly altered. 3. Indomethacin (6 x 106 M) increased the sensitivity of the skin to applied prostaglandin E1. The other prostaglandin synthetase inhibitors did not have this effect. Indomethacin (6 x 106 M) also enhanced the effect of antidiuretic hormone on the short-circuit current. 4. Indomethacin (30 x 106 M) increased the short-circuit current and diminished the response to applied prostaglandin E1. 5. In sulphate Ringer, theophylline increased the short-circuit current and diminished the response to prostaglandin E1. 6. Prostaglandin E1 increased the levels of cyclic AMP in frog skin and these increases preceded the increases in short-circuit current. There was a seasonal variation in the level of cyclic AMP in the skin: the levels in winter exceeded those in summer. There was also a seasonal variation in the cyclic AMP response to prostaglandin E1: the winter response was greater than that in summer. 7. Indomethacin (6 x 106 M) had a biphasic effect on cyclic AMP levels in the skin, an initial increase followed by a decrease. Indomethacin also potentiated prostaglandin E1 stimulated cyclic AMP accumulation. 8. Theophylline increased cyclic AMP levels in the skin and potentiated prostaglandin E1 stimulated cyclic AMP accumulation. W. J. HALL AND OTHERS 9. Pre-treatment of the skin with theophylline reversed the effects of cyclic AMP on the short-circuit current and open-circuit potential. 10. It is concluded that endogenous prostaglandins help to maintain sodium transport across isolated frog skin and that the effects of E-type prostaglandins on the short-circuit current are mediated by increased cyclic AMP levels. The transient increase in short-circuit current and the increased skin sensitivity caused by indomethacin (6 x 10-6 mI) are attributed to inhibition of phosphodiesterase activity. The failure of theo-phylline to potentiate the short-circuit current response of the skin to prostaglandin E1 is attributed to alteration of cyclic AMP action on the skin by theophylline.

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“…The influence of mucosal anions on sodium transport in amphibian epithelia has been reported in numerous instances (Zadunaisky & de Fisch, 1963;Aceves, Erlij & Edwards, 1968;Ferreira, 1968;Cuthbert, Painter & Prince, 1969). The influence of anions on sodium transport in the Amphiuma bladder resembles that observed in the turtle bladder (Gonzalez, Shamoo & Brodsky, 1969).…”

Section: Discussionmentioning

confidence: 80%

The electrical properties and active ion transport across the urinary bladder of the urodele, Amphiuma means.

Degnan¹,

Zadunaisky²

1977

The Journal of Physiology

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SUMMARY1. The electrical properties and the active transport processes of the isolated urinary bladder of the urodele, Amphiuma means, were studied by mounting this tissue as a flat sheet between two halves 'of a lucite chamber. The mean transepithelial potential difference was 70-2 + 2-3 mV (serosa positive), the mean short-circuit current was 10*9 + 05 ,uA/mg of dry weight and the mean transepithelial d.c. resistance was 6540 + 374 Q. mg of dry weight.2. The short-circuit current (he) accounted for 92 % of the net 22Na+ flux from the mucosa to the serosa. The difference resulted from a transport of 36Cl-in the same direction as sodium.3. The active sodium transport exhibited typical saturation kinetics, having a Km of 15-4 m-equiv/l. and approaching zero order at 60-70 mequiv/l. The transepithelial potential difference increased linearly with the log of the mucosal sodium concentration at a rate of 50 3 mV per tenfold concentration change.4. In the absence of the major anions (HCO3-and Cl-) from the bathing solutions, the electrical properties and the sodium influx decreased to less than 40 % of their control values. The presence of only one of these two. anions in the serosal bathing solution was sufficient to maintain these, parameters.5. Amiloride (10-5 M) and ouabain (106 M) inhibited the sodium transport 97 %/0 and 85 % respectively. Amphotericin B (106 M) stimulated the. sodium transport 47 %. Furosemide (10-3 M) inhibited the chloride transport 43 %. The sodium transport was insensitive to the action of two enurohypophyseal peptides tested, lysine-vasotocin and pitocin.

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The effects of anions on sodium transport

Cited by 16 publications

References 12 publications

Effect of anions on amiloride-sensitive, active sodium transport across rabbit colon,in Vitro

Effect of anions on amiloride-sensitive, active sodium transport across rabbit colon,in Vitro

Endogenous prostaglandins, adenosine 3':5'‐monophosphate and sodium transport across isolated frog skin.

The electrical properties and active ion transport across the urinary bladder of the urodele, Amphiuma means.

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