Purpose: In this study, we investigated physical and psychological effects of the shiatsu (finger pressure) stimulation in a sitting position. Subjects and Methods: A crossover test was conducted on 20 subjects (average 34.5 ± 7.6 years old; male = 10, female = 10). First they were randomly divided into two groups: Group A (shiatsu stimulation/control) and group B (control/shiatsu stimulation). They had shiatsu stimulation (Namikoshi basic methods in the sitting position, five minutes). At the same time, POMS (profile of mood states), SBP (systolic blood pressure, DBP (diastolic blood pressure) and HR (heart rate) were measured. Results: Physical result-Compared to the baseline, the shiatsu stimulation showed statistical decreased SBP and HR while the control (no shiatsu stimulation) did not. Psychological result (POMS)-Compared to the baseline, the stimulation showed significant change in all six mood states (Tension-Anxiety, Depression-Dejection, Fatigue and Confusion decreased while Vigor elevated). Male showed statistical decrease in Tension-Anxiety, Depression-Dejection, Anger, Fatigue and Confusion as well as significant increase in Vigor. Female only showed decrease in Depression-Dejection and Anger. Discussion and Conclusion: The shiatsu stimulation in the sitting position could modulate autonomic nerve system and it could affect human body. Gender differences were found in physiological effect.