The effects of bioactive compounds from blueberry and blackcurrant powders on the inhibitory activities of oat bran pastes against α-amylase and α-glucosidase linked to type 2 diabetes

Hui, Xiaodan; Wu, Gang; Han, Didi; Stipkovits, Letitia; Wu, Xiyang; Tang, Shuze; Brennan, Margaret A.; Brennan, Charles S.

doi:10.1016/j.foodres.2020.109756

Cited by 55 publications

(40 citation statements)

References 31 publications

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“…Similarly, D3G (100 µM) exhibited the inhibition of α-glucosidase by 44.5% [42]. Based on a previous study, the delphinidin-3-glucoside showed the most potency of α-glucosidase inhibition and was followed by cyanidin-3-glucoside, cyanidin-3-rutinoside, and malvidin-3-glucoside [43]. A previous study reported that anthocyanins could inhibit the intestinal α-glucosidase by the competitive inhibition between the glycosyl groups of anthocyanins and the active site of enzymes [44].…”

Section: Discussionmentioning

confidence: 89%

“…There also found that the number of hydroxyl groups in the B-ring of anthocyanins associated with an increase of binding affinity to the enzyme [45]. Moreover, the inhibitory effect of C3G and D3G on α-glucosidase also depends on the binding affinity with the enzyme and their chemical structure [43]. According to the molecular docking study, the binding of D3G and α-glucosidase depends on the polar interaction, hydrogen bonding, and hydrophobic interaction in the enzyme's catalytic site [42].…”

Section: Discussionmentioning

confidence: 94%

“…It is noteworthy that the molecular docking predicted D3G could bind with amino acid residues (Gly402 and Val380) of α-glucosidase through hydrogen bonding. In the meantime, C3G showed the binding affinity with amino residues, including Glu231, Val335, Gly402, and Val380 [43]. Therefore, it is hypothesized that ABE could inhibit α-glucosidase (maltase and sucrase) through the interaction of enzymes resulting from cyanidin-3-glucoside and delphinidin-3-glucoside as a predominant anthocyanin in ABE.…”

Section: Discussionmentioning

confidence: 99%

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Inhibitory Effect of Antidesma bunius Fruit Extract on Carbohydrate Digestive Enzymes Activity and Protein Glycation In Vitro

et al. 2020

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Antidesma bunius (L.) spreng (Mamao) is widely distributed in Northeastern Thailand. Antidesma bunius has been reported to contain anthocyanins, which possess antioxidant and antihypertensive actions. However, the antidiabetic and antiglycation activity of Antidesma bunius fruit extract has not yet been reported. In this study, we investigated the inhibitory activity of anthocyanin-enriched fraction of Antidesma bunius fruit extract (ABE) against pancreatic α-amylase, intestinal α-glucosidase (maltase and sucrase), protein glycation, as well as antioxidant activity. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) chromatogram revealed that ABE contained phytochemical compounds such as cyanidin-3-glucoside, delphinidin-3-glucoside, ellagic acid, and myricetin-3-galactoside. ABE inhibited intestinal maltase and sucrase activity with the IC50 values of 0.76 ± 0.02 mg/mL and 1.33 ± 0.03 mg/mL, respectively. Furthermore, ABE (0.25 mg/mL) reduced the formation of fluorescent AGEs and the level of Nε-carboxymethyllysine (Nε-CML) in fructose and glucose-induced protein glycation during four weeks of incubation. During the glycation process, the protein carbonyl and β-amyloid cross structure were decreased by ABE (0.25 mg/mL). In addition, ABE exhibited antioxidant activity through DPPH radical scavenging activity and Trolox equivalent antioxidant capacity (TEAC) with the IC50 values 15.84 ± 0.06 µg/mL and 166.1 ± 2.40 µg/mL, respectively. Meanwhile, ferric reducing antioxidant power (FRAP) showed an EC50 value of 182.22 ± 0.64 µg/mL. The findings suggest that ABE may be a promising agent for inhibiting carbohydrate digestive enzyme activity, reducing monosaccharide-induced protein glycation, and antioxidant activity.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

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Inhibitory Effect of Antidesma bunius Fruit Extract on Carbohydrate Digestive Enzymes Activity and Protein Glycation In Vitro

et al. 2020

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“…Anthocyanin-containing food extracts (2 g/day human equivalents) have also been shown to reduce insulin resistance in estrogen-deficient rats [ 15 ]. Anthocyanins demonstrate competitive inhibitory activity against α-amylases and α-glucosidases like acarbose by binding to their active sites and have shown been to lower postprandial glucose concentrations in molecular docking analyses [ 19 ]. Therefore, aqueous blackcurrant extract intake of about 1 to 2 g/day may encourage antidiabetic activity by improving insulin sensitivity and suppressing α-amylase activity.…”

Section: Discussionmentioning

confidence: 99%

“…Their major bioactive components are cyanidin 3- O -rutinoside and delphinidin 3- O -rutinoside, and they have also been reported to have anti-inflammatory, immunomodulatory, antioxidant, and antimicrobial properties [ 17 ]. They also demonstrate α-amylase and α-glucosidase inhibitory activities and stimulate AMPK activity to improve glucose metabolism [ 15 , 19 ]. Previous studies have shown that intake of 600 mg of anthocyanin-rich blackcurrant 30 min before a high carbohydrate meal reduces serum glucose concentration and elevates gastric inhibitory peptide (GIP) and glucagon-like peptide-1 (GLP-1) in post-menopausal women and men [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Aqueous Blackcurrant Extract Improves Insulin Sensitivity and Secretion and Modulates the Gut Microbiome in Non-Obese Type 2 Diabetic Rats

Yang

Zhang

et al. 2021

Antioxidants

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This study was undertaken to determine whether aqueous blackcurrant extracts (BC) improve glucose metabolism and gut microbiomes in non-obese type 2 diabetic animals fed a high-fat diet and to identify the mechanism involved. Partially pancreatectomized male Sprague–Dawley rats were provided a high-fat diet containing 0% (control), 0.2% (L-BC; low dosage), 0.6% (M-BC; medium dosage), and 1.8% (H-BC; high dosage) blackcurrant extracts; 0.2% metformin (positive-C); plus 1.8%, 1.6%, 1.2%, 0%, and 1.6% dextrin, specifically indigestible dextrin, daily for 8 weeks. Daily blackcurrant extract intakes were equivalent to 100, 300, and 900 mg/kg body weight (bw). After a 2 g glucose or maltose/kg bw challenge, serum glucose and insulin concentrations during peak and final states were obviously lower in the M-BC and H-BC groups than in the control group (p < 0.05). Intraperitoneal insulin tolerance testing showed that M-BC and H-BC improved insulin resistance. Hepatic triglyceride deposition, TNF-α expression, and malondialdehyde contents were lower in the M-BC and H-BC groups than in the control group. Improvements in insulin resistance in the M-BC and H-BC groups were associated with reduced inflammation and oxidative stress (p < 0.05). Hyperglycemic clamp testing showed that insulin secretion capacity increased in the acute phase (2 to 10 min) in the M-BC and H-BC groups and that insulin sensitivity in the hyperglycemic state was greater in these groups than in the control group (p < 0.05). Pancreatic β-cell mass was greater in the M-BC, H-BC, and positive-C groups than in the control group. Furthermore, β-cell proliferation appeared to be elevated and apoptosis was suppressed in these three groups (p < 0.05). Serum propionate and butyrate concentrations were higher in the M-BC and H-BC groups than in the control group. BC dose-dependently increased α-diversity of the gut microbiota and predicted the enhancement of oxidative phosphorylation-related microbiome genes and downregulation of carbohydrate digestion and absorption-related genes, as determined by PICRUSt2 analysis. In conclusion, BC enhanced insulin sensitivity and glucose-stimulated insulin secretion, which improved glucose homeostasis, and these improvements were associated with an incremental increase of the α-diversity of gut microbiota and suppressed inflammation and oxidative stress.

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Biological activities, therapeutic potential, and pharmacological aspects of blackcurrants (Ribes nigrum L): A comprehensive review

Ejaz,

Waliat,

Afzaal

et al. 2023

Food Science & Nutrition

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Blackcurrant possesses various health‐endorsing attributes owing to its polyphenol profile. Recent studies have demonstrated its therapeutic potential against various health disorders. Various bioactives present in blackcurrants have different functional and pharmacological aspects including anti‐inflammatory, antioxidant, and antimicrobial properties. The most dominant and important bioactive include anthocyanins, flavonols, phenolic acids, and polyunsaturated fatty acids. Food formats derived from blackcurrants comprise pomace, juice, powder, and extracts. All these food formats have industrial, prebiotic, and pharmacological benefits. In the current article, the nutritional composition, industrial applications, and therapeutic potential are discussed in the recent literature. Moreover, novel extraction techniques for the extraction of bioactive compounds present in blackcurrants and their safety concerns have been elaborated.

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The effects of bioactive compounds from blueberry and blackcurrant powders on the inhibitory activities of oat bran pastes against α-amylase and α-glucosidase linked to type 2 diabetes

Cited by 55 publications

References 31 publications

Inhibitory Effect of Antidesma bunius Fruit Extract on Carbohydrate Digestive Enzymes Activity and Protein Glycation In Vitro

Inhibitory Effect of Antidesma bunius Fruit Extract on Carbohydrate Digestive Enzymes Activity and Protein Glycation In Vitro

Aqueous Blackcurrant Extract Improves Insulin Sensitivity and Secretion and Modulates the Gut Microbiome in Non-Obese Type 2 Diabetic Rats

Biological activities, therapeutic potential, and pharmacological aspects of blackcurrants (Ribes nigrum L): A comprehensive review

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