The effects of calcium phosphate cement particles on osteoblast functions

Pioletti, Dominique P.; Takei, Hiroshi; Lin, Tong; Landuyt, P. Van; Jun, Quing; Kwon, Soon Yong; Sung, K.L. Paul

doi:10.1016/s0142-9612(99)00250-1

Cited by 125 publications

(85 citation statements)

References 36 publications

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“…Pioletti et al described that CaP cement particles smaller than 10 mm have an effect on the viability and proliferation of osteoblast-like cells. 40 Evidently, the observed acidity of Calcibon CaP cement in in vitro cell culture experiments does not hamper the final in vivo behavior of the material, because in vivo studies with Calcibon 21,24,41 describe excellent bone compatibility, as characterized by the fast deposition of new bone on the cement surface, which proves that the material is osteoconductive and suitable for clinical purposes. A reason for the excellent in vivo behavior might be vascularization, which provides a continuous flow around the inserted implant that can elute the toxicity of the CaP cement.…”

Section: Discussionmentioning

confidence: 96%

The Cytocompatibility and Early Osteogenic Characteristics of an Injectable Calcium Phosphate Cement

et al. 2007

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In this study, the cytocompatibility and early osteogenic characteristics of rat bone marrow cells (RBMCs) on injectable calcium phosphate (CaP) cement (Calcibon) were investigated. In addition to unmodified CaP cement discs, 2 other treatments were given to the discs: preincubation in MilliQ and sintering at different temperatures. After primary culture, RBMCs were dropwise seeded on the discs and cultured for 12 days. The samples were evaluated in terms of cell viability, morphology (live and dead assays and scanning electron microscopy (SEM)), cell proliferation (deoxyribonucleic acid (DNA) analyses), early cell differentiation (alkaline phosphatase (ALP) activity), and physicochemical analyses (xray diffraction (XRD)). The live and dead, DNA, and SEM results showed that Calcibon discs without any additional treatment were not supporting osteoblast-like cells in vitro. There were fewer cells, and cell layers were detached from the disc surface. Therefore, different preincubation periods and sintering temperatures were evaluated to improve the cytocompatibility of the CaP cement. Preincubating discs in MilliQ for periods of 1, 4, 8, and 12 weeks resulted in the hydrolysis of a-tri calcium phosphate (TCP) into an apatite-like structure with some b-TCP, as shown with XRD, but the material was not cytocompatible. Sintering the discs between 8008C and 11008C resulted in conversion of a-TCP to b-TCP with some hydroxyapatite and an increase in crystallinity. Eventually, the discs sintered at 11008C achieved better cell attachment, more-abundant cell proliferation, and earlier differentiation than other sintered (6008C, 8008C, and 10008C), preincubated, and unmodified specimens. On basis of our results, we conclude that in vivo results with CaP-based cements do not guarantee in vitro applicability. Furthermore, unmodified Calcibon is not cytocompatible in vitro, although preincubation of the material results in a more-favorable cell response, sintering of the material at 11008C results in the best osteogenic properties. In contrast to in vivo studies, the Calcibon CaP cement is not suitable as a scaffold for cellbased tissue-engineering strategies.

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Section: Discussionmentioning

confidence: 96%

The Cytocompatibility and Early Osteogenic Characteristics of an Injectable Calcium Phosphate Cement

et al. 2007

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“…Titanium (Ti) particles downregulated the gene expression of type I collagen [8], while UHMWPE particles were shown to affect osteoblast differentiation [9]. We showed that particles had a cytotoxic effect [10], modulated the fibronectin gene expression [11], and decreased the adhesion strength [12] of osteoblasts. Moreover, combination of particles and cytokines had a synergic effect on the production of inflammatory factors [13].…”

Section: Introductionmentioning

confidence: 92%

The influence of wear particles in the expression of osteoclastogenesis factors by osteoblasts

Pioletti

Kottelat

2004

Biomaterials

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Orthopedic implant failures are often associated with peri-implant osteolysis. Particles generated from the wear process have been suspected to play an important role in this situation. Indeed, the peri-implant osteolysis could be due to the presence of particles stimulating the osteoclastogenesis process. We hypothesize then that the presence of a low particle concentration positively influences osteoblasts to produce osteoclastogenesis factors. If true, this hypothesis would then support the idea that the particles could be at the origin of the process leading to implant loosening. To check the validity of this hypothesis, we quantified in vitro the production of different genes involved in the osteoclastogenesis process using primary isolated human osteoblasts treated or not with particles. Results showed that low concentrations of particles might have a stimulating effect on osteoblasts to produce osteoclastogenesis factors as demonstrated by the increase of RANKL and CSF-1 gene expression in the particle group. r

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“…3 These particles are generated through years of wear. Any of the materials (metallic, polyethylene, methacrylate, ceramic) used for the implants or even the calcium phosphate cement 4 can be involved. In cases of loose Ti implants, the mean concentration of Ti particles retrieved from tissue surrounding the implants can reach 0.1% of the dry tissue weight.…”

Section: Introductionmentioning

confidence: 99%

Gene expression analysis of osteoblastic cells contacted by orthopedic implant particles

Pioletti

Leoni

Genini

et al. 2002

J. Biomed. Mater. Res.

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Particles generated from orthopedic implants through years of wear play an essential role in the aseptic loosening of a prosthesis. We have investigated the biocompatibility of these orthopedic particles on different osteoblast-like cells representative of different stages of osteoblast maturation. We found the particles induced a caspasedependent apoptosis of osteoblasts, with less mature osteoblasts being the most susceptible. An analysis of gene expression was performed on the less mature osteoblasts, which were in contact with the particles. We found that the particles had a profound impact on genes that code for inflammatory cytokines and genes involved in controlling the nuclear architecture. Results from this study suggest that the peri-implant osteolysis after a total joint replacement can be due in part to a decrease of bone formation and not solely to an overstimulation of bone resorption as is generally proposed. Development of new drugs that promote normal bone formation and osteoblast survival would possibly control peri-implant osteolysis, resulting in a better prognosis for patients with orthopedic implants.

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The effects of calcium phosphate cement particles on osteoblast functions

Cited by 125 publications

References 36 publications

The Cytocompatibility and Early Osteogenic Characteristics of an Injectable Calcium Phosphate Cement

The Cytocompatibility and Early Osteogenic Characteristics of an Injectable Calcium Phosphate Cement

The influence of wear particles in the expression of osteoclastogenesis factors by osteoblasts

Gene expression analysis of osteoblastic cells contacted by orthopedic implant particles

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