The Effects of Carbendazim on Acute Toxicity, Development, and Reproduction in Caenorhabditis elegans

Abstract

Carbendazim, as a fungicide, was commonly used to control fungal diseases in agriculture, forestry, and veterinary medicines. In this study, the acute and reproductive toxicity of carbendazim was assessed using Caenorhabditis elegans (C. elegans) as a model in order to preliminarily evaluate the potential risks of this fungicide in agricultural production and application. The results showed that the growth of C. elegans was inhibited by 0.01 μg/L carbendazim. The treatment of 0.1 μg/L carbendazim caused a significant decrease in locomotion behavior and significant damage to the reproductive and antioxidant system, causing the lifespan of nematodes to be drastically shortened. These results provide a better understanding of the environmental risk of carbendazim and raise new concerns about safety.

Carbendazim toxicity in different cell lines and mammalian tissues

“…[15][16][17] Despite the fact that CBZ toxicity has been known since the 1980s, it has recently become an important topic due to the growing concern as environmental endocrine disruptor. [18] CBZ induces multiple defects in various experimental species. CBZ causes toxicity by blocking the development of microtubules in the neurological system, as well as the male reproductive and endocrine systems, and is a known teratogen.…”

“…[22,[24][25][26] CBZ has been banned in European countries due to its detrimental environmental and health effects including developmental and reproductive disruptions, toxicity and long term adverse effects. [4,13,18] Despite the fact that toxicological evidence led the US Environmental Protection Agency to suspend their registrations for a decade, CBZ is still widely used in agriculture in several countries including India, Brazil and China. CBZ has a residual half-life of 7.0 and 6.5 days in entire fruits after preharvest and postharvest treatments, respectively.…”

Polysaccharides from Volvariella volvacea inhibit fat accumulation in C. elegans dependent on the aak‐2/nhr‐49‐mediated pathway

“…Worms were grown on nematode growth medium (NGM) plates and fresh E. coli OP50 was sprayed as the food source. Synchronized L1 worms were applied in all assays, which were synchronized by bleach and NaOH followed by washing with M9 buffer three times according to previously described (Bai et al, 2020; Li et al., 2020). Lyophilized VPS was diluted ddH 2 O at a 10 mg/ml of stocking solution, which was detected by the phenol–sulfuric acid method.…”

التأثير الوقائي لمستخلص فاکهة الکمکوات الإيثانولي ضد تسمم الکبد الناتج عن الکاربيندازيم في الفئران

“…Carbendazim is a known aneugen (5) and can be classified as a possible human carcinogen (6). In rats, carbendazim has been shown to have negative effects on biochemical, histological, and hematological markers in the liver, kidney, and endocrine glands, as well as their hormonal levels (7). Kumquat is a small, elliptical shaped fruit, closely related to citrus.…”