The effects of carbon/phosphorus ratio on polyphosphate- and glycogen-accumulating organisms in aerobic granular sludge

Muszyński, Adam; Miłobędzka, Aleksandra

doi:10.1007/s13762-015-0828-8

Cited by 31 publications

(5 citation statements)

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“…These moderate PR efficiencies could have been caused by the low biodegradability of tannic acid, which has been previously assessed [ 14 , 16 ]. A previous study [ 30 ] reported that polyphosphate accumulating organisms (PAO) accounted for 70% of all bacteria from AGS when the ratio COD/P was 15:1, which was associated with a high phosphorus release/dissolved organic carbon uptake ratio (0.4). The opposite trend was observed when the COD/P ratio was 100:1, favoring the glycogen accumulating organisms (GAO), which compete with PAO for a carbon source.…”

Section: Resultsmentioning

confidence: 99%

Treatment of old landfill leachate with high ammonium content using aerobic granular sludge

et al. 2017

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BackgroundAerobic granular sludge has become an attractive alternative to the conventional activated sludge due to its high settling velocity, compact structure, and higher tolerance to toxic substances and adverse conditions. Aerobic granular sludge process has been studied intensively in the treatment of municipal and industrial wastewater. However, information on leachate treatment using aerobic granular sludge is very limited.MethodsThis study investigated the treatment performance of old landfill leachate with different levels of ammonium using two aerobic sequencing batch reactors (SBR): an activated sludge SBR (ASBR) and a granular sludge SBR (GSBR). Aerobic granules were successfully developed using old leachate with low ammonium concentration (136 mg L−1 NH4 +-N).ResultsThe GSBR obtained a stable chemical oxygen demand (COD) removal of 70% after 15 days of operation; while the ASBR required a start-up of at least 30 days and obtained unstable COD removal varying from 38 to 70%. Ammonium concentration was gradually increased in both reactors. Increasing influent ammonium concentration to 225 mg L−1 N, the GSBR removed 73 ± 8% of COD; while COD removal of the ASBR was 59 ± 9%. The GSBR was also more efficient than the ASBR for nitrogen removal. The granular sludge could adapt to the increasing concentrations of ammonium, achieving 95 ± 7% removal efficiency at a maximum influent concentration of 465 mg L−1 N. Ammonium removal of 96 ± 5% was obtained by the ASBR when it was fed with a maximum of 217 mg L−1 NH4 +-N. However, the ASBR was partially inhibited by free-ammonia and nitrite accumulation rate increased up to 85%. Free-nitrous acid and the low biodegradability of organic carbon were likely the main factors affecting phosphorus removal.ConclusionThe results from this research suggested that aerobic granular sludge have advantage over activated sludge in leachate treatment.

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Section: Resultsmentioning

confidence: 99%

Treatment of old landfill leachate with high ammonium content using aerobic granular sludge

et al. 2017

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“…Competibacter abundance increased by >40% with the increasing rbCOD/P ratios from 20 to 50, but abundance of DF2 did not seem to vary, indicating that Competibacter was likely the one that responded to the change in loading ratio. Muszyński and Miłobędzka (2015) also observed increase in Competibacter abundance from 4 to 20% when the rbCOD/P ratio was changed from 15:1 to 100:1 with aerobic granular sludge while abundance of Alphaproteobacterial Defluvicoccus cluster 1 ( DF1 ) remained constant at 2% (they did not detect DF2 type GAOs in the granular sludge). These results clearly showed that both relative abundance and community compositions of PAOs and GAOs shifted as the influent rbCOD/P ratio changed.…”

Section: Resultsmentioning

confidence: 84%

Impact of Influent Carbon to Phosphorus Ratio on Performance and Phenotypic Dynamics in Enhanced Biological Phosphorus Removal (EBPR) System - Insights into Carbon Distribution, Intracellular Polymer Stoichiometry and Pathways Shifts

Majed

2019

Preprint

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13This study investigated the impact of influent carbon to phosphorus (P) ratio on the variation in 14 P-removal performance and associated intracellular polymers dynamics in key functionally 15 relevant microbial populations, namely, PAOs and GAOs, at both individual and populations 16 levels, in laboratory scale sequencing batch reactor-EBPR systems. Significant variations and 17 dynamics were evidenced for the formation, utilization and stoichiometry of intracellular 18 polymers, namely polyphosphate, glycogen and Polyhydroxyalkanoates in PAOs and GAOs in 19 the EBPR systems that were operated with influent C/P ranged from 20 to 50, presumably as 20 results of phylogenetic diversity changes and, or metabolic functions shifts in these two 21 populations at different influent C/P ratios. Single cell Raman micro-spectroscopy enabled 22 quantification of differentiated polymer inclusion levels in PAOs and GAOs and, showed that as 23 the influent rbCOD/P ratio increases, the excessive carbon beyond stoichiometric requirement 24 for PAOs would be diverted into GAOs. Our results also evidenced that when condition becomes 25 more P limiting at higher rbCOD/P ratios, both energy and reducing power generation required 26 2 for acetate uptake and PHB formation might shift from relying on both polyP hydrolysis and 1 glycolysis pathway, to more enhancement and dependence on glycolysis in addition to 2 partial/reverse TCA cycle. These findings provided new insights into the metabolic elasticity of 3 PAOs and GAOs and their population-level parameters for mechanistic EBPR modeling. This 4 study also demonstrated the potential of application of single cell Raman micro-spectroscopy 5 method as a powerful tool for studying phenotypic dynamics in ecological systems such as 6 EBPR. 7KEYWORDS Enhanced biological phosphorus removal, EBPR, Raman microscopy, 8 polyphosphate, PHB, glycogen 9 10 phosphorus removal, the benefits are often offset, in practice, by the needs to have standby 1 chemicals for achieving reliable and consistent performance. There is still knowledge gap in 2 understanding the mechanism and factors that control the stability of the process, particularly for 3 achieving extremely low effluent limits and, as a result, process stability and system performance 4 have been seen to vary among facilities (Gu et al., 2005; Neethling et al., 2005; Stephens et al., 5 2004). 6EBPR performance and stability have been shown to be affected by many factors among 7 which, the competition of the two main functionally relevant populations, namely polyphosphate 8 accumulating organisms or PAOs and glycogen accumulating organisms or GAOs, were found 9 to be crucial for achieving successful operation of EBPR. Although deterioration of EBPR 10 performance has been attributed to the proliferation of GAOs in lab-scale EBPRs and also at full 11 scale EBPR facilities (Gu et al., 2005; Cech and Hartman, 1993; Saunders et al., 2003), others 12 (Gu et al., 2008; Tu and Schuler, 2013) have also shown that efficient EBPR could a...

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“…Furthermore, Competibacter plays an important role in AGS formations. It has been identified as a GAO and has been found in AGS (de Kreuk & van Loosdrecht 2004;Weissbrodt et al 2013;Muszynśki & Miłobedzka 2015).…”

Section: Granulationmentioning

confidence: 99%

A dynamically controlled anaerobic/aerobic granular sludge reactor efficiently treats brewery/bottling wastewater

Vleeschauwer

Caluwé

Dobbeleers

et al. 2021

Water Science and Technology

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This study investigated the application of a dynamic control strategy in an aerobic granular sludge (AGS) reactor treating real variable brewery/bottling wastewater. For 482 days, the anaerobic and aerobic reaction steps in a lab-scale AGS system were controlled dynamically. A pH-based control was used for the anaerobic step, and an oxygen uptake rate (OUR) based control for the aerobic step. Additionally, the effect of an elongated aerobic step, and the effect of the removal of the suspended solids from the influent, on AGS formation were also investigated. In comparison to a static operation, the dynamic operation resulted in similar reactor performance, related to effluent quality and the anaerobic dissolved organic carbon (DOC) uptake efficiency, while the organic loading rate was significantly higher. The removal of suspended solids from the influent by chemical coagulation with FeCl3 turned hybrid floccular-granular sludge into fully granular sludge. The granulation coincided with a significant increase in the abundance of the glycogen-accumulating Candidatus Competibacter and an increase in the content of gel-forming EPS to respectively around 14% and 30%. In conclusion, this study showed the successful application of a dynamic control strategy based on common and low-cost sensors for AGS treatment of industrial wastewater.

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The effects of carbon/phosphorus ratio on polyphosphate- and glycogen-accumulating organisms in aerobic granular sludge

Cited by 31 publications

References 34 publications

Treatment of old landfill leachate with high ammonium content using aerobic granular sludge

Treatment of old landfill leachate with high ammonium content using aerobic granular sludge

Impact of Influent Carbon to Phosphorus Ratio on Performance and Phenotypic Dynamics in Enhanced Biological Phosphorus Removal (EBPR) System - Insights into Carbon Distribution, Intracellular Polymer Stoichiometry and Pathways Shifts

A dynamically controlled anaerobic/aerobic granular sludge reactor efficiently treats brewery/bottling wastewater

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