The effects of CD147 on the cell proliferation, apoptosis, invasion, and angiogenesis in glioma

Abstract

To analyze the effects of extracellular matrix metalloproteinase inducer (CD147) on glioma proliferation, apoptosis, invasion, and angiogenesis. Tissue samples were obtained from 101 glioma cases while normal brain tissues were obtained from 30 brain injury cases. Immunohistochemical assay was performed to detect the expressions of CD147, CD34, and VEGF in tissue samples. QRT-PCR was performed to detect the relative expression of CD147 mRNA in human glioma cell lines. CD147 siRNA was transfected into glioma cell line U251. Cell proliferation, apoptosis, invasion, and angiogenesis were tested by MTT, flow cytometry, Transwell assay, and vasculogenic mimicry assay, respectively. Expressions of relative proteins were analyzed with western blot. CD147 was positively expressed with the percentage of 0, 37.5, 44.8, 67.9, and 85.7 % in normal tissues and glioma tissues with WHO grades I-IV, respectively, and the scores of MVDand VEGF were associated with the expression of CD147. CD147 was significantly upregulated in the human glioma cell lines (P < 0.05). Downregulated the expression of CD147 suppressed cell proliferation, blocked cell cycle, induced apoptosis, inhibited cell invasion and angiogenesis in glioma cells in vitro. The expression of CD147 was significantly associated with WHO tumor grade and angiogenesis; silencing of CD147 contributed to inhibition of glioma proliferation, invasion, and angiogenesis. Our study provided firm evidence that CD 147 is a potential glioma target for anti-angiogenic therapies.

Antibodies targeting surface membrane antigens in patients with chronic graft-versus-host disease

“…47 Similarly, blocking CD147 reduced tumor metastasis and prolonged survival in mouse models. [48][49][50][51][52] CD49b, CD49c, and CD49f (ITGA6) are cell surface receptor integrins known to play important roles in controlling the organization of cell cytoskeletons, cancer cell migration, and invasion. 53,54 Targeting these surface proteins has been shown to inhibit cancer metastasis.…”

Cerium Oxide Nanoparticles as Novel Tool in Glioma Treatment: An In vitro Study

“…As CNP impede migration; invasion and tube formation of ECs; our findings suggest an inhibition or decrease of the release of soluble (pro-invasive) factors by the studied cells. In our study; we focussed on the cell adhesion molecule EMMPRIN (CD147; Basigin) known to play a leading role in proliferation; invasion; and angiogenesis in brain tumors such as gliomas [11,13]. Indeed; further experiments showed that the amount of EMMPRIN in the conditioned medium of CNP-treated cells was significantly lowered compared to the mock-treated controls.…”

“…The protein EMMPRIN; also known as CD147 or basigin; belongs to the immunoglobulin family of adhesion molecules and is a type I transmembrane glycoprotein [10]. Epidemiological studies show that EMMPRIN is overexpressed in about 67 to 86% of anaplastic astrocytoma and glioblastoma; therefore often used as negative prognostic factor for patients with astrocytic gliomas [11][12][13]. In addition to its occurrrence on the surface and as secreted form of tumor cells [14][15][16]; EMMPRIN expression was also described for endothelial cells [17][18][19] and was shown to have a broad range of functions.…”

The Combination of CD147 and MMP-9 Serum Levels Is Identified as Novel Chemotherapy Response Markers of Advanced Non-Small-Cell Lung Cancer

“…With regard to the heterogeneity of tumor microenvironments, CD147 can activate matrix metalloproteinases (MMPs), degrade the extracellular matrix (ECM) of tumor cells, and induce the invasion/metastases of tumor cells [9,10]. CD147 also plays a pivotal role in inhibiting the apoptosis of tumor cells [11].…”