The effects of cold atmospheric plasma on cell adhesion, differentiation, migration, apoptosis and drug sensitivity of multiple myeloma

Xu, Dehui; Luo, Xiaohui; Xu, Yujing; Cui, Qingjie; Yang, Yanjie; Liu, Dingxin; Chen, Hailan; Kong, Michael G.

doi:10.1016/j.bbrc.2016.04.027

Cited by 56 publications

(35 citation statements)

References 56 publications

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“…C). Studies have shown that NTAP treatment reduces the expression and activities of MMP‐2 and MMP‐9 on thyroid papillary cancer cells and on multiple myeloma cells . Although no living tissues were evaluated in the present study, the down‐regulation of metalloproteinases by NTAP might be a promising tool for using to avoid enzymatic degradation of dentin over time.…”

Section: Discussionmentioning

confidence: 87%

Effect of non‐thermal atmospheric plasma on the dentin‐surface topography and composition and on the bond strength of a universal adhesive

Ayres

Bonvent

Mogilevych

et al. 2017

European J Oral Sciences

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This study investigated the effect of application of non-thermal atmospheric plasma (NTAP) on the topography and composition of the dentin surface, as well as the microtensile bond strength (μTBS) of a universal adhesive to NTAP-treated dentin. Exposed flat dentin surfaces from human third molars were either treated with NTAP for 10 and 30 s or untreated (control). The dentin-surface topography and chemical composition were characterized by atomic force microscopy (n = 3) and Raman confocal spectroscopy (n = 5), respectively. The μTBS (n = 8) of Scotchbond Universal to dentin was determined after storage for 24 h and 1 yr, either by direct water exposure or under simulated pulpal pressure. In-situ zymography was used to evaluate the influence of NTAP on the dentin-enzymatic activity. Non-thermal atmospheric plasma produced no remarkable topographical or chemical alterations at the dentin surface; only the amount of phosphate decreased following 10 s of treatment with NTAP. After 1 yr of direct water exposure, the μTBS of NTAP-treated specimens did not differ statistically significantly from that of untreated controls, whereas simulated pulpal pressure-aging resulted in a significantly higher μTBS for NTAP-treated dentin. The dentin-enzymatic activity appeared to be treatment-dependent, but the untreated controls showed more intense fluorescence within the hybrid layer. Scotchbond Universal maintained its μTBS strength after 1 yr of direct water exposure and simulated pulpal pressure, although remarkable statistical differences between treatments were observed depending on the aging condition.

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Section: Discussionmentioning

confidence: 87%

Effect of non‐thermal atmospheric plasma on the dentin‐surface topography and composition and on the bond strength of a universal adhesive

Ayres

Bonvent

Mogilevych

et al. 2017

European J Oral Sciences

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“…There is evidence from the recent literature to suggest that CAP shows a strong anti-cancer effects in a wide range of cancer cell lines, including melanoma, oral carcinoma, breast cancer, lung cancer and myeloma ( Fridman et al, 2007 ; Kim et al, 2010a ; Kim et al, 2010b ; Kim et al, 2010c ; Chang et al, 2014 ; Adachi et al, 2015 ; Vermeylen et al, 2016 ; Xu et al, 2016 ). Moreover, CAP has shown the ability to suppress or eradicate subcutaneous xenograft tumors in several mouse models ( Yajima et al, 2014 ; Mirpour et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Gene expression profiling and functional analysis reveals that p53 pathway-related gene expression is highly activated in cancer cells treated by cold atmospheric plasma-activated medium

Shi

Feng

et al. 2017

PeerJ

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BackgroundCold atmospheric-pressure plasma (CAP) has been considered a promising strategy for anti-cancer treatment. Traditionally, CAP was employed to kill cancer cells or tumor tissues by direct irradiation. However, CAP has some disadvantages such as infiltration capacity and storage convenience. Recently, plasma-activated medium (PAM) was used as an alternative strategy to treat cancer cells or tumors. The novel PAM approach has potential as an anti-cancer therapy.ObjectiveTo reveal the global activation of signaling pathways in oral cancer cells induced by PAM.MethodsOral squamous cell line SCC15 were treated by PAM and gene expression profiles were evaluated by using RNA-seq. Functional analyses were employed to reveal the global responses of SCC15 cells with PAM stimulation. QRT-PCR and Western blot were carried out to validate the expression levels of selected genes.ResultsMore than 6G clean data per sample were obtained in PAM-treated SCC15 cells. A total of 934 differentially expressed genes (DEGs) were identified and GO analysis implicated the deep involvement of biological process. KEGG mapping further clustered 40 pathways, revealing that “p53 pathway” was significantly enriched. SCC15 cells were commonly used as a p53-null cell line. Therefore, the enriched p53 pathway-related genes in our analysis might be activated by other stimulators, in a p53-independent manner. Gene set enrichment analysis (GSEA) was also performed to evaluate changes at the gene-sets level. The results demonstrated not only the high engagement of “p53 pathway” but also the involvement of novel pathways such as hypoxia pathway.ConclusionsThe present study elucidates the transcriptomic changes of PAM treated SCC15 cells, containing highly enriched DEGs involved in “p53 pathway”. Our analysis in this work not only provides genomic resources for future studies but also gives novel insights to uncover the molecular mechanism of PAM stimulation.

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“…The geometrical parameters of these plasma jets and the materials used to assembly the device are very numerous [5]. Thus sometimes the biological effects of plasma jets are strongly conflicting, being even opposite in certain studies [6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Time Behaviour of Helium Atmospheric Pressure Plasma Jet Electrical and Optical Parameters

et al. 2017

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Low temperature plasma jets gained increased interest in the last years as a potential device in many life science applications, including here human or veterinary medicine. Standardisation of plasma sources and biological protocols are necessary for quality assurance reasons, due to the fact that this type of atmospheric pressure plasma source is available in multiple configurations and their operational parameters span also on a broad range of items, such as all characteristics of high voltage pulses used for gas breakdown, geometrical characteristics, gas feed composition and conductive or biological target characteristics. In this paper we present results related to electrical, optical and molecular beam mass spectrometry diagnosis of a helium plasma jet, emphasising the influence of various operational parameters of the high voltage pulses on plasma jet properties. Discussion on physical parameters that influence the biological response is included, together with important results on plasma sources statistical behaviour until reaching a quasi-stationary working regime. The warm-up period of the plasma jet, specific to many other plasma sources, must be precisely known and specified whenever the plasma jets are used as a tool for life science applications.

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The effects of cold atmospheric plasma on cell adhesion, differentiation, migration, apoptosis and drug sensitivity of multiple myeloma

Cited by 56 publications

References 56 publications

Effect of non‐thermal atmospheric plasma on the dentin‐surface topography and composition and on the bond strength of a universal adhesive

Effect of non‐thermal atmospheric plasma on the dentin‐surface topography and composition and on the bond strength of a universal adhesive

Gene expression profiling and functional analysis reveals that p53 pathway-related gene expression is highly activated in cancer cells treated by cold atmospheric plasma-activated medium

Time Behaviour of Helium Atmospheric Pressure Plasma Jet Electrical and Optical Parameters

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