The effects of collector geometry on the internal structure of the 3D nanofiber scaffold fabricated by divergent electrospinning

Zhou, Yingge; Hu, Zhiyong; Du, Dong; Tan, George Z.

doi:10.1007/s00170-018-2899-4

Cited by 22 publications

(10 citation statements)

References 46 publications

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“…Functionalized electrospun fibrous scaffolds incorporate specific compounds like drugs, biologically active species as well as other biocompatible chemical agents that can improve the accommodation of specific cell types [ 22 , 23 , 24 ]. Apart from composition diversity, these structures can provide specific cues for cells surface guidance, based on custom tailored scaffolds topography [ 25 , 26 ], as well as 3D architectures to closely resemble the native tissue microstructures [ 27 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

Poly(ε-caprolactone) Titanium Dioxide and Cefuroxime Antimicrobial Scaffolds for Cultivation of Human Limbal Stem Cells

Trcin

Zdraveva

Dolenec³

et al. 2020

Polymers

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Limbal Stem Cell Deficiency (LSCD) is a very serious and painful disease that often results in impaired vision. Cultivation of limbal stem cells for clinical application is usually performed on carriers such as amniotic membrane or surgical fibrin gel. Transplantation of these grafts is associated with the risk of local postoperative infection that can destroy the graft and devoid therapeutic benefit. For this reason, electrospun scaffolds are good alternatives, as proven to mimic the natural cells surroundings, while their fabrication technique is versatile with regard to polymer functionalization and scaffolds architecture. This study considers the development of poly(ε-caprolactone) (PCL) immune-compatible and biodegradable electrospun scaffolds, comprising cefuroxime (CF) or titanium dioxide (TiO2) active components, that provide both bactericidal activity against eye infections and support of limbal stem cells growth in vitro. The PCL/CF scaffolds were prepared by blend electrospinning, while functionalization with the TiO2 particles was performed by ultrasonic post-processing treatment. The fabricated scaffolds were evaluated in regard to their physical structure, wetting ability, static and dynamic mechanical behaviour, antimicrobial efficiency and drug release, through scanning electron microscopy, water contact angle measurement, tensile testing and dynamic mechanical analysis, antimicrobial tests and UV-Vis spectroscopy, respectively. Human limbal stem cells, isolated from surgical remains of human cadaveric cornea, were cultured on the PCL/CF and PCL/TiO2 scaffolds and further identified through immunocytochemistry in terms of cell type thus were stained against p63 marker for limbal stem cells, a nuclear transcription factor and cytokeratin 3 (CK3), a corneal epithelial differentiation marker. The electrospun PCL/CF and PCL/TiO2 successfully supported the adhesion, proliferation and differentiation of the cultivated limbal cells and provided the antimicrobial effect against Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans.

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Section: Introductionmentioning

confidence: 99%

Poly(ε-caprolactone) Titanium Dioxide and Cefuroxime Antimicrobial Scaffolds for Cultivation of Human Limbal Stem Cells

Trcin

Zdraveva

Dolenec³

et al. 2020

Polymers

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“…Scaffolds made of micro-nanofibers have shown great potential for tissue engineering. In our previous research projects, a novel divergence electrospinning strategy was developed to scale up traditional 2D nanofiber mats to 3D nanofiber scaffolds with gradient microstructures along the vertical direction [ 32 , 33 , 34 , 35 , 36 ]. The scaffolds’ thicknesses ranged from 2 to 10 cm.…”

Section: Discussionmentioning

confidence: 99%

Fabrication of Nanopores Polylactic Acid Microtubes by Core-Sheath Electrospinning for Capillary Vascularization

2021

Self Cite

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There has been substantial progress in tissue engineering of biological substitutes for medical applications. One of the major challenges in development of complex tissues is the difficulty of creating vascular networks for engineered constructs. The diameter of current artificial vascular channels is usually at millimeter or submillimeter level, while human capillaries are about 5 to 10 µm in diameter. In this paper, a novel core-sheath electrospinning process was adopted to fabricate nanoporous microtubes to mimic the structure of fenestrated capillary vessels. A mixture of polylactic acid (PLA) and polyethylene glycol (PEO) was used as the sheath solution and PEO was used as the core solution. The microtubes were observed under a scanning electron microscope and the images were analyzed by ImageJ. The diameter of the microtubes ranged from 1–8 microns. The diameter of the nanopores ranged from 100 to 800 nm. The statistical analysis showed that the microtube diameter was significantly influenced by the PEO ratio in the sheath solution, pump rate, and the viscosity gradient between the sheath and the core solution. The electrospun microtubes with nanoscale pores highly resemble human fenestrated capillaries. Therefore, the nanoporous microtubes have great potential to support vascularization in engineered tissues.

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“…3D porous scaffolds are not only used as structural molds for tissue generation, but they also present signaling cues to cells and facilitate the delivery of therapeutic agents and oxygen. Generally, compared to conventional 2D scaffolds, the 3D porous scaffolds are capable of providing a better connection between organs and single cells 14‐17 . Up until the present time, various methods have been proposed for the fabrication of 3D fiber structures through electrospinning such as collector modifications, 17,18 self‐assembly processes, 19,20 needleless electrospinning, 21 conducting electrospinning under wet conditions, 22 the addition of porogen, 23 multilayering processes, and coaxial electrospinning.…”

Section: Introductionmentioning

confidence: 99%

“…Generally, compared to conventional 2D scaffolds, the 3D porous scaffolds are capable of providing a better connection between organs and single cells 14‐17 . Up until the present time, various methods have been proposed for the fabrication of 3D fiber structures through electrospinning such as collector modifications, 17,18 self‐assembly processes, 19,20 needleless electrospinning, 21 conducting electrospinning under wet conditions, 22 the addition of porogen, 23 multilayering processes, and coaxial electrospinning. Nonetheless, the aforementioned techniques may be costly or labor‐intensive as they involve the manipulation of the device or the collector 11,12 .…”

Section: Introductionmentioning

confidence: 99%

Biopolymer based three‐dimensional biomimetic micro/nanofibers scaffolds with porous structures via tailored charge repulsions for skin tissue regeneration

Rad

Mokhtari

Abbasi

2021

Polymers for Advanced Techs

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Nowadays, the fabrication of three‐dimensional (3D) fibrous scaffolds that mimic the natural skin tissue in microstructure and composition is still a challenge. 3D nanocomposite scaffolds combining biopolymers (e.g., proteins and polysaccharides) are promising candidates for skin tissue engineering. For this purpose, nano‐microfibers and nanofibers/net 3D scaffolds based on zein, gum arabic (GA), Calendula officinalis (C. officinalis) extract, and poly (ε‐caprolactone) (PCL) were successfully fabricated via electrospinning technique. The morphological observation of scaffolds indicated cone‐like construction. 3D structures were formed based on repulsions between adjacent fibers and high relative humidity. The results confirmed interconnected porous structure for the sintered 3D scaffolds. Physicochemical and biological properties of the fibrous scaffolds such as mechanical behavior, water sorption capacity, weight loss, porosity, and biocompatibility were studied. The 3D scaffolds revealed significant hydrophilicity and high porosity (ca. 94% porosity) with an average pore size >9 μm. In vitro biological evaluation indicated that PCL/Zein/GA/C. officinalis 3D scaffold with nanofibers/net morphology provided outstanding cell proliferation and attachment. The results confirmed that these nanofibers/net scaffolds with 3D structures could be potentially used in repairing skin defects.

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The effects of collector geometry on the internal structure of the 3D nanofiber scaffold fabricated by divergent electrospinning

Cited by 22 publications

References 46 publications

Poly(ε-caprolactone) Titanium Dioxide and Cefuroxime Antimicrobial Scaffolds for Cultivation of Human Limbal Stem Cells

Poly(ε-caprolactone) Titanium Dioxide and Cefuroxime Antimicrobial Scaffolds for Cultivation of Human Limbal Stem Cells

Fabrication of Nanopores Polylactic Acid Microtubes by Core-Sheath Electrospinning for Capillary Vascularization

Biopolymer based three‐dimensional biomimetic micro/nanofibers scaffolds with porous structures via tailored charge repulsions for skin tissue regeneration

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