The effects of combined low level laser therapy and mesenchymal stem cells on bone regeneration in rabbit calvarial defects

Fekrazad, Reza; Ghuchani, Mostafa Sadeghi; Eslaminejad, Mohammadreza Baghban; Taghiyar, Leila; Kalhori, Katayoun Am; Pedram, Mir Sepehr; Shayan, Arman Mohammadi; Aghdami, Nasser; Abrahamse, Heidi

doi:10.1016/j.jphotobiol.2015.08.002

Cited by 47 publications

(45 citation statements)

References 27 publications

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“…The process of studies inclusion/exclusion. be distinguished by specific laser such as GaAs, 37 GaAlAs, 29,[38][39][40][41] InGaAlP, 42,43 He-Ne, 44,45 and Nd:YAG. 35,46 Laser type was not available in two studies.…”

Section: Resultsmentioning

confidence: 99%

“…18 LEDs offer advantages such as low price, acceptable efficacy, high switching rate, and long life. 19,20 PBM has various biostimulatory effects on wound healing, [21][22][23][24] extracellular matrix synthesis, 25 and promotion of cellular proliferation and differentiation in tissues such as bone, [26][27][28][29] nerves, 30 and skin. [31][32][33][34][35][36] Studies in this area are few and contradictory.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Photobiomodulation on Mesenchymal Stem Cells

Fekrazad

Asefi

Allahdadi³

et al. 2016

Photomedicine and Laser Surgery

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Photobiomodulation on Mesenchymal Stem Cells

Fekrazad

Asefi

Allahdadi³

et al. 2016

Photomedicine and Laser Surgery

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“…Histomorphometric analysis revealed significant differences in favor of Group AFGL when compared to Group AFG at all postoperative periods, with a thinner interface. The neovascularization and thermic and biological stimuli provided by LLLT can lead to better graft integration [32,[35][36][37][38].…”

Section: Discussionmentioning

confidence: 99%

Effects of low-level laser therapy on autogenous bone graft stabilized with a new heterologous fibrin sealant

Gonçalves

Buchaim

Bueno³

et al. 2016

Journal of Photochemistry and Photobiology B: Biology

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Autogenous bone grafts are used to repair bone defects, and the stabilization is needed for bone regeneration. Laser photobiomodulation is a modality of treatment in clinical practice for tissue regeneration, and it has therapeutic effects as an anti-inflammatory, analgesic and modulating cellular activity. The aim of the present study was to evaluate the effects of low-level laser therapy (LLLT) on an autogenous bone graft integration process stabilized with a new heterologous fibrin sealant. Forty rats were divided into two groups: Autogenous Fibrin Graft (AFG, n=20), in which a 5mm dome osteotomy was conducted in the right parietal bone and the graft was adhered to the left side using fibrin sealant; and Autogenous Fibrin Graft Laser (AFGL, n=20), which was subjected to the same procedures as AFG with the addition of LLLT. The treatment was performed immediately following surgery and then three times a week until euthanasia, using an 830nm laser (30mW, 6J/cm(2), 0.116cm(2), 258.6mW/cm(2), 2.9J). Five animals from each group were euthanized at 10, 20, 30 and 40days postoperative, and the samples were submitted to histomorphological and histomorphometric analysis. Partial bone regeneration occurred, with new bone tissue integrating the graft to the recipient bed and small areas of connective tissue. Comparative analysis of the groups at the same intervals revealed minor interfaces in group AFGL, with statistically significant differences (p<0.05) at all of the analyzed intervals (10days p=0.0087, 20days p=0.0012, 30days p<0.0001, 40days p=0.0142). In conclusion, low-level laser therapy stimulated bone regeneration and accelerated the process of integration of autogenous bone grafts.

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“…The cranium of each animal was shaved and disinfected with povidone-iodine, and the surgical site was injected with 1 mL of 2% lidocaine HCL and 1:100,000 epinephrine (Yu-Han Co., Gunpo, Korea). An about 30 mm incision was made on the skull to expose the parietal bones, and four 6-mm-diameter calvarial defects were produced on each rabbit using a dental-trephine bur as described previously [34,35,36]. The same amounts (0.03 ± 0.002 g) of BCP (Bio-C, Cowellmedi, Pusan, Korea), BCP/CMC, BCP/c-CMC, and BCP/HyA were randomly placed on each calvarial defect (Figure 2).…”

Section: Methodsmentioning

confidence: 99%

The Effect of Bisphasic Calcium Phosphate Block Bone Graft Materials with Polysaccharides on Bone Regeneration

et al. 2017

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In this study, bisphasic calcium phosphate (BCP) and two types of polysaccharide, carboxymethyl cellulose (CMC) and hyaluronic acid (HyA), were used to fabricate composite block bone grafts, and their physical and biological features and performances were compared and evaluated in vitro and in vivo. Specimens of the following were prepared as 6 mm diameter, 2 mm thick discs; BPC mixed with CMC (the BCP/CMC group), BCP mixed with crosslinked CMC (the BCP/c-CMC group) and BCP mixed with HyA (the BCP/HyA group) and a control group (specimens were prepared using particle type BCP). A scanning electron microscope study, a compressive strength analysis, and a cytotoxicity assessment were conducted. Graft materials were implanted in each of four circular defects of 6 mm diameter in calvarial bone in seven rabbits. Animals were sacrificed after four weeks for micro-CT and histomorphometric analyses, and the findings obtained were used to calculate new bone volumes (mm3) and area percentages (%). It was found that these two values were significantly higher in the BCP/c-CMC group than in the other three groups (p < 0.05). Within the limitations of this study, BCP composite block bone graft material incorporating crosslinked CMC has potential utility when bone augmentation is needed.

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The effects of combined low level laser therapy and mesenchymal stem cells on bone regeneration in rabbit calvarial defects

Cited by 47 publications

References 27 publications

Effect of Photobiomodulation on Mesenchymal Stem Cells

Effect of Photobiomodulation on Mesenchymal Stem Cells

Effects of low-level laser therapy on autogenous bone graft stabilized with a new heterologous fibrin sealant

The Effect of Bisphasic Calcium Phosphate Block Bone Graft Materials with Polysaccharides on Bone Regeneration

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