The effects of composition and surface chemistry on the toxicity of quantum dots

Sun, Haizhu; Zhang, Fan; Wei, Haotong; Yang, Bai

doi:10.1039/c3tb21151g

Cited by 67 publications

(45 citation statements)

References 81 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, for long-term imaging and sensing, the performance of fluorescent proteins and organic dyes are significantly affected by their low photo-bleaching threshold. For example, although silica or polymer coating technique has promoted the colloidal stability of Qdots, the heavy metal ions present in Qdots would generate toxic effect on living organisms or biological cells [9][10][11][12]. Interestingly, semiconducting polymer dots (Pdots), as a new kind of nanomaterials with excellent photostability and low biological toxicity, have shown its promise in cell imaging, drug delivery and release, and disease diagnosis [1,13,14].…”

Section: Introductionmentioning

confidence: 98%

Bright red-emitting polymer dots for specific cellular imaging

et al. 2015

View full text Add to dashboard Cite

A new conjugated polymer, poly[{2-methoxy-5-(2-ethylhexyloxy)-1,4-(1-cyanovinylphenylene)}-alt-co-{2,5-bis(N,N 0 -diphenylamino)-1,4-phenylene}] (DB-CN-PPV), with strong UV absorption and high fluorescence in red wavelength region, is self-assembled into nanoparticles (polymer dots, Pdots) in aqueous solution upon rapid mixing of the organic solution with water. Different-sized Pdots were obtained by varying the organic solvent percent in the mixture solution and further characterized by dynamic light scattering and transmission electron microscopy. Spectroscopic measurements indicate that the DB-CN-PPV Pdots exhibit a bright red-emitting and a quantum yield of *27 %. Single-particle imaging and analyses indicate the brightness of DB-CN-PPV Pdots under 405 nm excitation is four times brighter than that of the well-known inorganic quantum dot probes, Qdot655, under same excitation light. The successful bioconjugation and cellular imaging suggest DB-CN-PPV Pdots have potential application for biological fluorescence imaging.

show abstract

Section: Introductionmentioning

confidence: 98%

Bright red-emitting polymer dots for specific cellular imaging

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The previous studies have demonstrated that surface modification of QDs is very important to reduce toxicity and in particular, coating with BSA could provide protection from photooxidation and improve the biocompatibility of QDs. 15,42 In our study, the tailor-designed amphiphilic BSA-PCL conjugate has been proven to have good biocompatibility and a high degree of safety, 21 and surface engineering of CIS/ZnS QDs with the prepared BSA-PCL conjugate imparts good biocompatibility to QDs. Building on the above-mentioned MTT results, it can be deduced that the constructed CIS/ZnS nanoprobe is highly biocompatible and nontoxic to the living cells.…”

Section: Scheme 1 Construction Of the Nir Fluorescence Nanoprobe Witmentioning

confidence: 72%

“…12,13 Therefore, rational surface engineering and functionalization of CIS/ZnS QDs are of great significance. 14,15 Until now, various strategies for surface modification of QDs have been developed. Among them, coating of QDs with amphiphilic polymers via hydrophobic interactions is considered to be a promising, relatively easy, and robust approach.…”

Section: Introductionmentioning

confidence: 99%

Facile Construction of Near Infrared Fluorescence Nanoprobe with Amphiphilic Protein-Polymer Bioconjugate for Targeted Cell Imaging

Liu

Chen

Dong

et al. 2015

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

A simple, straightforward, and reproducible strategy for the construction of a near-infrared (NIR) fluorescence nanoprobe was developed by coating CuInS 2 /ZnS quantum dots (CIS/ZnS QDs) with a novel amphiphilic bioconjugate. The amphiphilic bioconjugate with a tailor-designed structure of bovine serum albumin (BSA) as the hydrophilic segment and poly(ε-caprolactone) (PCL) as the hydrophobic part was fabricated by chemical coupling the hydrophobic polymer chain to BSA via the maleimide−sulfhydryl reaction. By incorporating CIS/ZnS QDs into the hydrophobic cores of the self-assembly of BSA-PCL conjugate, the constructed NIR fluorescence nanoprobe exhibited excellent fluorescent properties over a wide pH range (pH 3−10) and a good colloidal stability in PBS buffer (pH = 7.4) with or without 10% fetal bovine serum. The presence of the outer BSA shell effectively reduced the nonspecific cellular binding and imparted high biocompatibility and low-toxicity to the probe. Moreover, the NIR fluorescence nanoprobe could be functionalized by conjugating cyclic Arg-Gly-Asp (cRGD) peptide, and the decorated nanoprobe was shown to be highly selective for targeted integrin α v β 3 -overexpressed tumor cell imaging. The feasibility of the constructed NIR fluorescence probe in vivo application was further investigated and the results demonstrated its great potential for in vivo imaging. This developed protocol for phase transfer of the CIS/ZnS QDs was universal and applicable to other nanoparticles stabilized with hydrophobic ligands.

show abstract

“…In addition to the passivating schemes referred to in Section and surface coatings to promote nanoparticle dispersion in solution, directly functional quantum dot coatings have been implemented to modify cellular behavior during or after particle uptake. One demonstrated bifunctional capping agent, capable of both stabilizing nanoparticles and offering the biomolecular carboxyl‐amine for subsequent functionalization, is l ‐cysteine.…”

Section: Coupling Topography and Chemistrymentioning

confidence: 99%

Engineering the Cell-Semiconductor Interface: A Materials Modification Approach using II-VI and III-V Semiconductor Materials

Bain

Ivanisevic

2014

Small

View full text Add to dashboard Cite

Developing functional biomedical devices based on semiconductor materials requires an understanding of interactions taking place at the material-biosystem interface. Cell behavior is dependent on the local physicochemical environment. While standard routes of material preparation involve chemical functionalization of the active surface, this review emphasizes both biocompatibility of unmodified surfaces as well as use of topographic features in manipulating cell-material interactions. Initially, the review discusses experiments involving unmodified II-VI and III-V semiconductors - a starting point for assessing cytotoxicity and biocompatibility - followed by specific surface modification, including the generation of submicron roughness or the potential effect of quantum dot structures. Finally, the discussion turns to more recent work in coupling topography and specific chemistry, enhancing the tunability of the cell-semiconductor interface. With this broadened materials approach, researchers' ability to tune the interactions between semiconductors and biological environments continues to improve, reaching new heights in device function.

show abstract

The effects of composition and surface chemistry on the toxicity of quantum dots

Cited by 67 publications

References 81 publications

Bright red-emitting polymer dots for specific cellular imaging

Bright red-emitting polymer dots for specific cellular imaging

Facile Construction of Near Infrared Fluorescence Nanoprobe with Amphiphilic Protein-Polymer Bioconjugate for Targeted Cell Imaging

Engineering the Cell-Semiconductor Interface: A Materials Modification Approach using II-VI and III-V Semiconductor Materials

Contact Info

Product

Resources

About