The Effects of Cu-doped TiO2 Thin Films on Hyperplasia, Inflammation and Bacteria Infection

Abstract

In the present work, different concentrations of Cu ion (1, 2, 5 and 10 wt %) were doped in the TiO2 film by a sol-gel method and dip coating process. The morphology of the Cu-doped TiO2 films were characterized by scanning electron microscopy (SEM) and the results showed that the doped Cu made no change to the TiO2 films. The nitric oxide (NO) release experiment showed that these Cu-doped surfaces showed the ability of catalytic decomposition of exogenous donor S-nitroso-N-acetyl-penicillamine (SNAP) to generate (NO). Based on fluorescence analysis and CCK-8 quantitative results, such films had the ability to inhibit smooth muscle cells adhesion, proliferation and migration with SNAP in vitro. The macrophage adhesion assay and anti-bacterial test proved that such Cu-doped TiO2 films also possessed anti-inflammatory and anti-bacterial abilities. All the abilities above showed positive correlation with the amounts of the doped Cu. This study suggested that the Cu-doped TiO2 films were capable of generating physiological levels of NO in the presence of endogenous donor S-nitrosothiols (RSNO), endowing the TiO2 films with anti-hyperplasia, anti-inflammatory and anti-bacterial abilities.

Surface Modification of Cardiovascular Stent Material 316L SS with Estradiol-Loaded Poly (trimethylene carbonate) Film for Better Biocompatibility

“…Then, the samples were picked up and washed with NS at 37 °C three times, and subsequently fixed with 4% paraformaldehyde (Sigma, Ontario, CA, USA) for 30 min at 25 °C. After a rinsed step, the HUASMC on each sample was stained with the rhodamine reagent for 15 min and observed under inverted fluorescence microscopy (OLIMPUS-IX51, Japan) [ 36 ]. The HUASMC numbers of 316L SS, PTMC and PTMC-E5 were determined via a typical CCK-8 assay [ 36 ].…”

“…After a rinsed step, the HUASMC on each sample was stained with the rhodamine reagent for 15 min and observed under inverted fluorescence microscopy (OLIMPUS-IX51, Japan) [ 36 ]. The HUASMC numbers of 316L SS, PTMC and PTMC-E5 were determined via a typical CCK-8 assay [ 36 ].…”

Preparing Sodium Alginate/Polyethyleneimine Spheres for Potential Application of Killing Tumor Cells by Reducing the Concentration of Copper Ions in the Lesions of Colon Cancer

“…We also found that SA hydrogel has good plasticity and biocompatibility in our previous work [12]. However, the porous structures of tens of micron scales on the surface of SA hydrogels may not be conducive to the implementation of this function: The porous connectivity structure of hydrogels may cause the exudation of inhaled copper ions and reduce the efficiency of carrying copper [13]; on the other hand, pores with a diameter of tens of micron-scales may allow most cells to migrate and aggregate, and the migrated cells will cause long-term hydrogel retention in the body, delaying the excretion of adsorbed copper ions outside [14]. In the previous research, polyethyleneimine (PEI) molecules were modified on the materials surface via layer-by-layer self-assembly and were proved to inhibit series of pathologically related cells of esophageal cancer [15,16].…”

Preparing a novel magnesium‐doped hyaluronan/polyethyleneimine nanoparticle to improve endothelial functionalisation

“…The fluorescence intensity of CD31 on each sample was calculated by the software ipwin32 [31]. An MTT method was also performed to investigate the influence of the MgNP on HUVEC growth [32]. The nitric oxide (NO) released from the HUVEC was determined by the Griess reagent method [33].…”