The effects of cytokinin types and their concentration on in vitro multiplication of sweet cherry cv. Lapins (Prunus avium L.)

Abstract

Determination of the most optimal types and concentrations of plant growth regulators as medium constituents is one of the most important aspects of successful micropropagation, among other in vitro factors. With the aim of optimization of in vitro multiplication of sweet cherry cv. Lapins the effect of following cytokinins has been studied: benzyladenine (BA), isopentenyl adenine (2iP), kinetin (KIN) and thidiazuron (TDZ) at concentrations of 1, 2, 5, 10 and 15μM, combined with auxine, indole-3-butyric acid (IBA) at concentrations of 0, 0.5, 2.5 and 5μM. Murashige and Skoog (1962) was the basic medium used in all the combinations. The following multiplication parameters were monitored: multiplication index, length of axial and lateral shoots. Fresh and dry shoot weight (callus, stem and leaves -S + L) were determined. Some specific issues, such as colour, leaf and callus size, leaf roll, incidence of chlorosis or necrosis along with occurrence of rhizogenesis, i.e. roots unusual for this phase of micropropagation, were also monitored. The highest multiplication index as well as length of axial and lateral shoots was obtained on media with BA. Very poor multiplication, with large sized shoots and big leaves, was achieved on media with 2iP, TDZ and KIN, whereas in many combinations with 2iP, and particularly in those with KIN, rhizogenesis was induced. Obtained results suggest that the choice of cytokinins for the phase of multiplication of sweet cherry is limited to BA. For more rapid micropropagation, through joining rooting and multiplication phases, KIN and 2iP may be applied. The latter two may be also used to obtain sturdy shoots (elongation phase, prior to rooting).

Micropropagation of carnation ( Dianthus caryophyllus L . ) in liquid medium by temporary immersion bioreactor in comparison with solid culture

“…In our study it was suggested that 3 mg L −1 BAP is the optimal plant growth regulators for shoot multiplication of carnation plantlets, due to better performance of 3 mg L −1 BAP on number of shoots and rate of proliferation than other concentrations. Considering this fact, it is highly recommended that the higher concentration of cytokinin component is necessary for the growth and differentiation of tissue culture [8]. It agrees with results obtained by Kadota and Niimi with pear ( Pyrus pyrifolia) [21] .…”

Naturally Occurring and Artificial N9-Cytokinin Conjugates: From Synthesis to Biological Activity and Back

“…Moreover, 6-benzylaminopurine (BAP) and Kin, which are widely used in many commercial tissue culture techniques [ 19 , 20 ], due to their low cost and high efficacy, are probably the most well-known ArCKs [ 21 ]. Benzyalminopurine is used for the micropropagation of vast numbers of plant species [ 22 , 23 , 24 , 25 , 26 , 27 ]. Kin has been usually used in mixtures with α-naphthalene acetic acid (NAA) in tissue culture of many plants as well [ 28 , 29 , 30 , 31 , 32 ].…”

Anti-neuroinflammatory potential of Tylophora indica (Burm. f) Merrill and development of an efficient in vitro propagation system for its clinical use

“…In contrast, we have established a more efficient protocol forming 12-13 shoots per nodal explants on both solid and liquid media. However, reduced rate of shoot proliferation at 15μM of KN may be due to its weak potential to induce shoot multiplication [49,50]. One of the major challenges involved in the scale-up of in vitro micropropagation method is the cost involved in the process.…”