The effects of d-Tyrosine combined with amikacin on the biofilms of Pseudomonas aeruginosa

She, Pengfei; Chen, Lihua; Liu, Hongbo; Zou, Yaru; Luo, Zhen; Koronfel, Asmaa; Wu, Yong

doi:10.1016/j.micpath.2015.07.009

Cited by 28 publications

(30 citation statements)

References 32 publications

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“…Thus, the use of disassembling agents is considered a good strategy to enhance the therapeutic potential of AMPs and other antimicrobials against mature biofilms. A number of compounds including lytic enzymes, furanones, triterpenes and D-amino acids have shown ability to disassemble the biofilm structure [ 46 , 47 , 48 , 49 ]. In this study, we used as potentiating agent the cation chelator EDTA.…”

Section: Discussionmentioning

confidence: 99%

The Semi-Synthetic Peptide Lin-SB056-1 in Combination with EDTA Exerts Strong Antimicrobial and Antibiofilm Activity against Pseudomonas aeruginosa in Conditions Mimicking Cystic Fibrosis Sputum

Maisetta

Grassi

Esin

et al. 2017

IJMS

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Pseudomonas aeruginosa is a major cause of chronic lung infections in cystic fibrosis (CF) patients. The ability of the bacterium to form biofilms and the presence of a thick and stagnant mucus in the airways of CF patients largely contribute to antibiotic therapy failure and demand for new antimicrobial agents able to act in the CF environment. The present study investigated the anti-P. aeruginosa activity of lin-SB056-1, a recently described semi-synthetic antimicrobial peptide, used alone and in combination with the cation chelator ethylenediaminetetraacetic acid (EDTA). Bactericidal assays were carried out in standard culture conditions and in an artificial sputum medium (ASM) closely resembling the CF environment. Peptide’s structure and interaction with large unilamellar vesicles in media with different ionic strengths were also investigated through infrared spectroscopy. Lin-SB056-1 demonstrated fast and strong bactericidal activity against both mucoid and non-mucoid strains of P. aeruginosa in planktonic form and, in combination with EDTA, caused significant reduction of the biomass of P. aeruginosa mature biofilms. In ASM, the peptide/EDTA combination exerted a strong bactericidal effect and inhibited the formation of biofilm-like structures of P. aeruginosa. Overall, the results obtained highlight the potential of the lin-SB056-1/EDTA combination for the treatment of P. aeruginosa lung infections in CF patients.

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Section: Discussionmentioning

confidence: 99%

The Semi-Synthetic Peptide Lin-SB056-1 in Combination with EDTA Exerts Strong Antimicrobial and Antibiofilm Activity against Pseudomonas aeruginosa in Conditions Mimicking Cystic Fibrosis Sputum

Maisetta

Grassi

Esin

et al. 2017

IJMS

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“…Effects of D-amino acids on B. subtilis may be simply due to toxic effects of inhibition of protein synthesis (48). Others have reported that mixtures of D-amino acids (49) or combinations of D-amino acids with antibiotics (50) may be more effective against biofilm formation than individual D-amino acids. Interpretation of these studies is complicated due to differences in strains, biofilm assays (for example, microtiter plate vs. flow-cell), and the dosage of specific D-amino acids used (51).…”

Section: Introductionmentioning

confidence: 99%

Bacterial d -amino acids suppress sinonasal innate immunity through sweet taste receptors in solitary chemosensory cells

et al. 2017

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In the upper respiratory epithelium, bitter and sweet taste receptors present in solitary chemosensory cells influence antimicrobial innate immune defense responses. Whereas activation of the bitter taste receptor (T2R) stimulates surrounding epithelial cells to release antimicrobial peptides, activation of the sweet taste receptor (T1R) in the same cells inhibits this response. It is thought that this mechanism exists to control the magnitude of antimicrobial peptide release based upon the sugar content of airway surface liquid. We hypothesized that D-amino acids, which are produced by various bacteria and activate T1R in taste receptor cells in the mouth, may also activate T1R in the airway. Here, we show that both the T1R2 and T1R3 subunits of the sweet taste receptor (T1R2/3) are present in the same chemosensory cells of primary human sinonasal epithelial cultures. Respiratory isolates of Staphylococcus species, but not Pseudomonas aeruginosa, produced at least two D-amino acids that activate the sweet taste receptor. In addition to inhibiting P. aeruginosa biofilm formation, D-amino acids derived from Staphylococcus inhibited T2R-mediated signaling and defensin secretion in sinonasal cells by activating T1R2/3. D-amino acid–mediated activation of T1R2/3 also enhanced epithelial cell death during challenge with Staphylococcus aureus in the presence of the bitter-receptor–activating compound denatonium benzoate. These data establish a potential mechanism for interkingdom signaling in the airway mediated by bacterial D-amino acids and the mammalian sweet taste receptor in airway chemosensory cells.

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“…The clinical isolates utilized in this study were selected from a collection of clinical isolates of P. aeruginosa during January 2014 to December 2014 (She et al. ) from the Third Xangya hospital of Centre South University (Changsha, Hunan, China). No special ethical permit was required for this study according to the Chinese law.…”

Section: Methodsmentioning

confidence: 99%

“…Pseudomonas aeruginosa strain PAO1 (ATCC 15692) is a well-characterized wound isolate widely used as a laboratory strain (Holloway 1955;Schmidt et al 1996). The clinical isolates utilized in this study were selected from a collection of clinical isolates of P. aeruginosa during January 2014 to December 2014 (She et al 2015) from the Third Xangya hospital of Centre South University (Changsha, Hunan, China). No special ethical permit was required for this study according to the Chinese law.…”

Section: Bacterial Strains and Growth Mediamentioning

confidence: 99%

Effects of norspermidine on Pseudomonas aeruginosa biofilm formation and eradication

She

Wang

et al. 2016

MicrobiologyOpen

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Biofilms are defined as aggregation of single cell microorganisms and associated with over 80% of all the microbial infections. Pseudomonas aeruginosa is a Gram‐negative opportunistic pathogen capable of leading to various infections in immunocompromised people. Recent studies showed that norspermidine, a kind of polyamine, prevented and disrupted biofilm formation by some Gram‐negative bacterium. In this study, the effects of norspermidine on P. aeruginosa biofilm formation and eradication were tested. Microtiter plate combined with crystal violet staining was used to study the effects of norspermidine on P. aeruginosa initial attachment, then we employed SEM (scanning electron microscope), qRT‐PCR, and QS‐related virulence factor assays to investigate how norspermidine prevent biofilm formation by P. aeruginosa. We reported that high‐dose norspermidine had bactericide effect on P. aeruginosa, and norspermidine began to inhibit biofilm formation and eradicate 24‐h mature biofilm at concentration of 0.1 and 1 mmol/L, respectively, probably by preventing cell‐surface attachment, inhibiting swimming motility, and downregulating QS‐related genes expression. To investigate the potential utility of norspermidine in preventing device‐related infections, we found that catheters immersed with norspermidine were effective in eradicating mature biofilm. These results suggest that norspermidine could be a potent antibiofilm agent for formulating strategies against P. aeruginosa biofilm.

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The effects of d-Tyrosine combined with amikacin on the biofilms of Pseudomonas aeruginosa

Cited by 28 publications

References 32 publications

The Semi-Synthetic Peptide Lin-SB056-1 in Combination with EDTA Exerts Strong Antimicrobial and Antibiofilm Activity against Pseudomonas aeruginosa in Conditions Mimicking Cystic Fibrosis Sputum

The Semi-Synthetic Peptide Lin-SB056-1 in Combination with EDTA Exerts Strong Antimicrobial and Antibiofilm Activity against Pseudomonas aeruginosa in Conditions Mimicking Cystic Fibrosis Sputum

Bacterial d -amino acids suppress sinonasal innate immunity through sweet taste receptors in solitary chemosensory cells

Effects of norspermidine on Pseudomonas aeruginosa biofilm formation and eradication

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