The effects of dental pulp stem cells on bone regeneration in rat calvarial defect model: Micro-computed tomography and histomorphometric analysis

Asutay, Fatih; Polat, Serkan; Gül, Mehmet; Subaşı, Cansu; Kahraman, Sibel; Karaöz, Erdal

doi:10.1016/j.archoralbio.2015.09.002

Cited by 40 publications

(35 citation statements)

References 31 publications

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“…hDPSCs are a kind of mesenchymal stem cell with the potential for cell-mediated therapy and tissue engineering applications. Previous work has reported that hDPSCs have significant osteogenic differentiation ability for bone regeneration ( Asutay et al., 2015 ; Khanna-Jain et al., 2012 ).…”

Section: Discussionmentioning

confidence: 99%

In vitro response of dental pulp stem cells in 3D scaffolds: A regenerative bone material

Jimenez

Munévar

González

et al. 2018

Heliyon

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Three-dimensional-porous scaffolds of bone graft substitutes play a critical role in both cell targeting and transplantation strategies. These scaffolds provide surfaces that facilitate the response of stem cells related to attachment, survival, migration, proliferation, and differentiation.ObjectiveThe aim of this study was to evaluate the in vitro behavior of human dental pulp mesenchymal stem cells cultured on scaffolds of polylactic/polyglycolic acid with and without hydroxyapatite.MethodWe performed an in vitro experimental study using dental pulp stem cells obtained from samples of premolars, molars. The cells were cultured on scaffolds with osteogenic differentiation medium. Cell proliferation, adhesion and cell differentiation to an osteoblastic linage in the biomaterial were evaluated at three different time points: 7, 15 and 30 days. Each experiment was performed in triplicate. Analysis of the data was performed with the Split Plot block and MANOVA model.ResultsThe differentiation capability of hDPSCs towards the osteoblast lineage was better in the scaffold of PLGA/HA at 7, 15 and 30 days, as indicated by the high expression of osteogenic markers RUNX2, ALP, OPN and COL-I, compared with differentiation in the PLGA scaffold. No statistically significant differences were found in cell adhesion between the two types of scaffolds.ConclusionThe PLGA/HA scaffold provided better physical and chemical signals, as judged by the ability of dental pulp stem cells to adhere, proliferate and differentiate toward the osteogenic lineage.

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Section: Discussionmentioning

confidence: 99%

In vitro response of dental pulp stem cells in 3D scaffolds: A regenerative bone material

Jimenez

Munévar

González

et al. 2018

Heliyon

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“…While no complete healing was detected after 8 weeks, more bone was formed in the DPSC group than in the two other control groups. In another experiment, DPSCs were mixed with HA/TCP paste and injected into full-thickness cranial defects of immuno-deficient rats [210]. The in vivo results showed that the calcification rate and bone mineral density values in the DPSC/HA/TCP group were significantly higher than in the other groups, suggesting that DPSCs may be a suitable cell source for bone tissue engineering.…”

Section: Applications Of Injectable Scaffolds In Dental and Craniomentioning

confidence: 99%

Injectable scaffolds: Preparation and application in dental and craniofacial regeneration

Chang

Ahuja

et al. 2017

Materials Science and Engineering: R: Reports

211

170

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Injectable scaffolds are appealing for tissue regeneration because they offer many advantages over pre-formed scaffolds. This article provides a comprehensive review of the injectable scaffolds currently being investigated for dental and craniofacial tissue regeneration. First, we provide an overview of injectable scaffolding materials, including natural, synthetic, and composite biomaterials. Next, we discuss a variety of characteristic parameters and gelation mechanisms of the injectable scaffolds. The advanced injectable scaffolding systems developed in recent years are then illustrated. Furthermore, we summarize the applications of the injectable scaffolds for the regeneration of dental and craniofacial tissues that include pulp, dentin, periodontal ligament, temporomandibular joint, and alveolar bone. Finally, our perspectives on the injectable scaffolds for dental and craniofacial tissue regeneration are offered as signposts for the future advancement of this field.

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“…The bone‐forming capacity of DPSC has been demonstrated in animal models and, as for bone marrow stromal stem cells, an appropriate scaffold and supplementary growth factors seem to be prerequisites for these cells to participate in bone regeneration in vivo (Asutay et al., ; Chamieh et al., ; Tsukamoto et al., ; Wongsupa, Nuntanaranont, Kamolmattayakul, & Thuaksuban, ). Notwithstanding the large number of animal preclinical studies, there are few human in vivo studies addressing the bone regenerative capacity of DPSC.…”

Section: Mesenchymal Stem Cells and Bone Regenerationmentioning

confidence: 99%

Mesenchymal stem cells and biologic factors leading to bone formation

Bartold

Gronthos

Haynes

et al. 2019

J Clinic Periodontology

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Background Physiological bone formation and bone regeneration occurring during bone repair can be considered distinct but similar processes. Mesenchymal stem cells (MSC) and associated biologic factors are crucial to both bone formation and bone regeneration. Aim To perform a narrative review of the current literature regarding the role of MSC and biologic factors in bone formation with the aim of discussing the clinical relevance of in vitro and in vivo animal studies. Methods The literature was searched for studies on MSC and biologic factors associated with the formation of bone in the mandible and maxilla. The search specifically targeted studies on key aspects of how stem cells and biologic factors are important in bone formation and how this might be relevant to bone regeneration. The results are summarized in a narrative review format. Results Different types of MSC and many biologic factors are associated with bone formation in the maxilla and mandible. Conclusion Bone formation and regeneration involve very complex and highly regulated cellular and molecular processes. By studying these processes, new clinical opportunities will arise for therapeutic bone regenerative treatments.

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The effects of dental pulp stem cells on bone regeneration in rat calvarial defect model: Micro-computed tomography and histomorphometric analysis

Cited by 40 publications

References 31 publications

In vitro response of dental pulp stem cells in 3D scaffolds: A regenerative bone material

In vitro response of dental pulp stem cells in 3D scaffolds: A regenerative bone material

Injectable scaffolds: Preparation and application in dental and craniofacial regeneration

Mesenchymal stem cells and biologic factors leading to bone formation

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