The effects of DGAT1 and DGAT2 mRNA expression on fat deposition in fatty and lean breeds of pig

Cui, Jingxiang; Zeng, Yongqing; Wang, H.; Chen, W.; Du, Jianguo; Chen, Q.M.; Hu, Yue; Yang, Lei

doi:10.1016/j.livsci.2011.04.007

Cited by 39 publications

(29 citation statements)

References 27 publications

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“…Total RNA was reverse transcribed to cDNA using SuperScrit TM III Reverse Transcriptase (Invitrogen) according to the manufacturer’s instructions (Omi et al 2005; Cui et al 2011). …”

Section: Methodsmentioning

confidence: 99%

Study on quantitative expression of PPARγ and ADRP in muscle and its association with intramuscular fat deposition of pig

et al. 2016

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BackgroundIntramuscular fat (intramuscular fat, IMF) is one of the important traits of pork quality. How to reasonably improve the intramuscular fat content is the most focus researchers. Some possible regulation of intramuscular fat deposition of candidate genes to cause the attention of people. The objective of this study was to elucidate the relationship between peroxisome proliferator-activated receptor γ (PPARγ) and adipose differentiation-related protein (ADRP) mRNA expression and intramuscular fat (IMF) deposition in the muscle tissue of three breeds of pig: Laiwu (LW), Lulai Black (LL), and Large White (LY).Results qPCR analysis of the PPARγ and ADRP genes in the three breeds of pig revealed PPARγ and ADRP mRNA expression profiles of LW > LL > LY and LL > LW > LY, respectively. PPARγ mRNA expression was significantly and positively correlated with IMF deposition (p < 0.05). There were significant correlations between PPARγ and ADRP mRNA expression levels (p < 0.01).ConclusionsThese results suggest correlations between PPARγ and ADRP in fat deposition and regulation in pigs, PPARγ gene may be a main effector of IMF content and play an important role during adipocyte differentiation in pigs, thereby providing new information to further elucidate molecular mechanisms associated with intramuscular fat deposition in Laiwu pigs and provides new data for further molecular studies of mechanisms underlying intramuscular fat deposition in human obesity. The continued elucidation of specific genetic mechanisms between PPARγ and ADRP warrants further studies.

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“…Total RNA was reverse transcribed to cDNA using SuperScrit TM III Reverse Transcriptase (Invitrogen) according to the manufacturer’s instructions (Omi et al 2005; Cui et al 2011). …”

Section: Methodsmentioning

confidence: 99%

Study on quantitative expression of PPARγ and ADRP in muscle and its association with intramuscular fat deposition of pig

et al. 2016

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“…It encodes an acyl CoA wax alcohol acyltransferase involved in sebum production, and in humans is expressed in sebocytes, aiding in the prevention of surface desiccation of the skin (Turkish et al 2005). The gene is a member of a family including DGAT1 and DGAT2 , which have previously been associated with backfat thickness and intramuscular fat content respectively in pig breeds (Cui et al 2011). It is likely that this pseudogenised AWAT1 in Duroc pigs represents a species-wide trait, as no transcripts for AWAT1 appear in the EST databases from any pig breeds.…”

Section: Resultsmentioning

confidence: 99%

The pig X and Y chromosomes: structure, sequence and evolution

Skinner

Sargent

Churcher

et al. 2014

Preprint

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We have generated an improved assembly and gene annotation of the pig X chromosome, and a first draft assembly of the pig Y chromosome, by sequencing BAC and fosmid clones, and incorporating information from optical mapping and fibre-FISH. The X chromosome carries 1,014 annotated genes, 689 of which are protein-coding. Gene order closely matches that found in Primates (including humans) and Carnivores (including cats and dogs), which is inferred to be ancestral. Nevertheless, several protein-coding genes present on the human X chromosome were absent from the pig (e.g. the cancer/testis antigen family) or inactive (e.g. AWAT1), and 38 pig-specific X-chromosomal genes were annotated, 22 of which were olfactory receptors. The pig Y chromosome assembly focussed on two clusters of male-specific low-copy number genes, separated by an ampliconic region including the HSFY gene family, which together make up most of the short arm. Both clusters contain palindromes with high sequence identity, presumably maintained by gene conversion. The long arm of the chromosome is almost entirely repetitive, containing previously characterised sequences. Many of the ancestral X-related genes previously reported in at least one mammalian Y chromosome are represented either as active genes or partial sequences. This sequencing project has allowed us to identify genes - both single copy and amplified - on the pig Y, to compare the pig X and Y chromosomes for homologous sequences, and thereby to reveal mechanisms underlying pig X and Y chromosome evolution.

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“…More recently, diacylgycerol acyltransferase (DGAT) mRNA expression has been demonstrated to be breed-dependent, and correlated to IMF content in Laiwu, Lulai Black, and Large White pigs (Cui et al, 2011). The highly positive correlation between the low density lipoprotein receptor (LDLR) and FABP3 expression and IMF content was also confirmed in porcine muscle in three genetic groups (Serão et al, 2011).…”

mentioning

confidence: 89%

Effects of breed, postnatal development, and nutrition on mRNA expression of the FTO gene in porcine muscle and its relationship with intramuscular fat deposition

Tao¹,

Men²,

Deng³

et al. 2013

CZECH J ANIM SCI

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ABSTRACT:The effects of breed, development, and nutrition on mRNA expression of the fat mass and obesityassociated gene (FTO) and its relationship with intramuscular fat (IMF) content in porcine muscle (m. longissimus dorsi; m.l.d.) were estimated. Purebred Jinhua, Zhongbai, Yorkshire, Duroc, Duroc × Zhongbai (DZ), and Duroc × Yorkshire × Landrace (DYL) pigs were used to investigate the effect of breed. Pigs weighing 2.5, 10, 20, 40, 60, and 100 kg were selected to study the effects of different stages of development. To study the effect of nutrition, four diets were selected: corn-soybean (CS), CS with 1.2% conjugated linoleic acid (CLA) or 0.05% creatine monohydrate (CMH), and barley-soybean (BS). All eighty animals were slaughtered, and m.l.d. samples were collected to examine FTO mRNA expression and IMF content. Results showed that breed significantly affected FTO mRNA expression and IMF content. FTO mRNA expression in the studied pigs was in the order: Zhongbai and Yorkshire > Duroc and DZ > Jinhua and DYL. The IMF content ordered by breed was Duroc > DZ > DYL > Jinhua > Zhongbai > Yorkshire. Both FTO mRNA expression and IMF content increased with age of the pigs, with the greatest difference seen between 100 kg pigs and all other weights. In the study, none of the four diets had a significant effect (P > 0.05) on FTO mRNA expression or IMF content. The study demonstrated that FTO mRNA expression increased with increasing body weight and was significantly affected by the breed of pigs. The results showed that FTO mRNA expression had an inconsistent correlation with IMF content between breeds and developmental ages.

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The effects of DGAT1 and DGAT2 mRNA expression on fat deposition in fatty and lean breeds of pig

Cited by 39 publications

References 27 publications

Study on quantitative expression of PPARγ and ADRP in muscle and its association with intramuscular fat deposition of pig

Study on quantitative expression of PPARγ and ADRP in muscle and its association with intramuscular fat deposition of pig

The pig X and Y chromosomes: structure, sequence and evolution

Effects of breed, postnatal development, and nutrition on mRNA expression of the FTO gene in porcine muscle and its relationship with intramuscular fat deposition

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