The effects of different bisphenol derivatives on oxidative stress, DNA damage and DNA repair in RWPE‐1 cells: A comparative study

Köse, Özge; Rachidi, Walid; Béal, David; Erkekoğlu, Pınar; Fayyad‐Kazan, Hussein; Koçer-Gümüşel, Belma

doi:10.1002/jat.3934

Cited by 39 publications

(27 citation statements)

References 73 publications

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“…GSH levels in this study were observed to be increase in the hepatocytes with concentrations that ranged between 15.63 and 250 µM; however, a signi cant decrease in the GSH content was determined with 500 µM of BPF. Similar to the results herein, Kose et al reported elevated GSH levels in RWPE-1 cells that were treated with BPA, as well as its analogues, BPF and BPS, for 24 h [16]. In another study, Maćczak et al [15] found depleted GSH levels in human erythrocytes exposed to BPF.…”

Section: Discussionsupporting

confidence: 86%

“…For example, cytotoxicity was increased on a dosedependent basis in human cell lines (HCLs), such as hepatoma HCL, HepG2; intestinal HCL, LS174T; and renal HCL, ACHN, treated with BPF concentrations ranging from 5 to 100 µM for 24 h [24]. Similarly, BPFinduced cytotoxicity was reported with concentrations of 0-600 µM for 24 h in RWPE-1 cells [16]. Russo et al reported that mouse embryo broblast cells and cancer cells exposed to BPF (0-300 µM for 48 h) resulted in increased cytotoxicity in a dose-dependent manner [25].…”

Section: Discussionmentioning

confidence: 93%

“…These mentioned members of the antioxidant defence system have been frequently selected as oxidative stress biomarkers that are related to xenobiotics in sh [14]. Toxicity studies in in vitro have shown that BPF increased ROS levels, induced lipid peroxidation, and modi ed antioxidant enzyme activity in different cell types, such as human erythrocytes [15], and RWPE-1 cells [16].…”

Section: Introductionmentioning

confidence: 99%

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In vitro effects of bisphenol F on antioxidant system indicators in the isolated hepatocytes of rainbow trout (Oncorhyncus mykiss)

Aykut

Kaptaner

2021

Mol Biol Rep

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Bisphenol F (BPF) has been used frequently in the plastics industry and the production of daily consumer products as an alternative to bisphenol A (BPA). It was aimed herein to determine the cytotoxic effects of BPF on hepatocytes isolated from the liver of rainbow trout (Oncorhyncus mykiss) using lactate dehydrogenase (LDH) assay and antioxidant defence system indicators. The cultured hepatocytes were exposed to seven concentrations (0, 15.63, 31.25, 62.50, 125, 250, and 500 µM) of BPF for 24 h. According to the LDH assay, the percentage of cytotoxicity was increased dose dependently in the cells.The malondialdehyde content, which is indicative of lipid peroxidation, was increased signi cantly at BPF concentrations between 15.63 and 250 µM, whereas it remained unchanged with a concentration of 500 µM. The activities of superoxide dismutase were increased, while those of catalase were decreased with all of the BPF concentrations. Elevated levels of reduced glutathione content were determined with BPF concentrations between 15.63 and 250 µM, but decreased signi cantly with a concentration of 500 µM.Signi cant increases in the activities of the glutathione peroxidase were found in hepatocytes treated with BPF at concentrations of 31.25 to 500 µM. GST activity was only signi cantly increased with a BPF concentration of 250 µM. The results showed that the toxic mechanism of BPF was mainly based on cell membrane damage and oxidative stress, which have an in uence on antioxidant defences. Therefore, BPF was reconsidered as a safe alternative instead of BPA in the manufacturing of industrial or daily products.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

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In vitro effects of bisphenol F on antioxidant system indicators in the isolated hepatocytes of rainbow trout (Oncorhyncus mykiss)

Aykut

Kaptaner

2021

Mol Biol Rep

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“…This genotoxic effect could be due to the potential of BPS to induce oxidative stress both in vivo ( 96 , 97 ) and in vitro ( 98 , 99 ). A modified version of the Comet assay demonstrated that BPS induced a genotoxic effect in human prostatic cells and that such effects were strictly associated to oxidative stress mechanisms ( 52 ).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, it is likely that BPS has the potential to interfere with, and disrupt the normal functions of endocrine system in organisms ( 51 ). Moreover, the genotoxic potential of BPS is also cause of concern since scientific literature reports conflicting results depending on the investigated experimental model ( 52 , 53 ). As the usage of BPS as a substitute to BPA is increasing there is an urgent need to improve our knowledge about possible adverse effects of BPS on physiological functions in living organisms.…”

Section: Introductionmentioning

confidence: 99%

Bisphenol A and Bisphenol S Induce Endocrine and Chromosomal Alterations in Brown Trout

et al. 2021

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Bisphenol A is a widely used compound found in large amount of consumer products. As concerns have been raised about its toxicological and public health effect, the use of alternatives to bisphenol A are now increasing. Bisphenol S is one of the analogues being used as a replacement for bisphenol A despite the fact that little is known about the effects of bisphenol S on living organisms. In this study, we investigated the potential endocrine and genotoxic effects of bisphenol A and bisphenol S in juvenile brown trout (Salmo trutta). The fish were exposed to the compounds for either 2 weeks or 8 weeks via sustained-release cholesterol implants containing doses of 2 mg/kg fish or 20 mg/kg fish of the substances. The effects on the thyroid hormone levels and the estrogenic disrupting marker vitellogenin were evaluated, along with the genotoxic markers micronucleated cells and erythrocyte nuclear abnormalities. An increase in plasma vitellogenin was observed in fish exposed to the high dose of bisphenol A for 2 weeks. At this experimental time the level of the thyroid hormone triiodothyronine (T3) in plasma was elevated after bisphenol S exposure at the high concentration, and paralleled by an increase of micronucleated cells. Moreover, bisphenol A induced an increase of micronuclei frequency in fish erythrocytes after the exposure at the lowest dose tested. Taken together the results indicate that both bisphenol A and its alternative bisphenol S cause endocrine disrupting and genotoxic effects in brown trout, although suggesting two different mechanisms of damage underlying bisphenol A and bisphenol S activity.

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Biochemical responses and antioxidant defense mechanisms in Channa Striatus exposed to Bisphenol S

Mohan,

Jacob,

Malini

et al. 2024

J Biochem & Molecular Tox

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Bisphenol S (BPS), a BPA analog and a safer alternative, is utilized in a diverse range of industrial applications, such as making polycarbonate plastics, epoxy resins, thermal receipt papers, and currency bills. Recently, the increased use of BPS in containers and packages for daily life has been interrogated due to its identical chemical structure and probable endocrine‐disrupting actions as BPA has. The present study aimed to evaluate the alterations in biochemical indices and antioxidant enzymes as certain indicators of the endocrine‐disrupting effect of BPS in Channa striatus, a freshwater fish. BPS‐exposed fish species were subjected to three sub‐lethal concentrations of BPS (1, 4, and 12 ppm) and observed after an interval of 7 and 21 days. Exposure to BPS caused a reduction in the level of protein in muscle, gonads and the liver due to an impairment of protein synthesis. Levels of cholesterol in the muscle, gonads, and liver of BPS‐exposed fish were found to be decreased after treatment, indicating either an inhibition of cholesterol biosynthesis in the liver or reduced absorption of dietary cholesterol. The levels of antioxidant enzymes such as superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase showed remarkable increases, while the activity of glutathione S‐transferase decreased considerably, indicating the antioxidant defense mechanism to counteract the oxidative stress induced by BPS. Moreover, a significant increase was noted in the level of lipid peroxidation products, like malondialdehyde and conjugate diene, which represent biomarkers of oxidative stress. The histoarchitecture changes were also observed in the liver, muscle and gonads of BPS‐treated fish species. The present study showed that sub‐lethal exposure to BPS significantly influenced the activities of these enzymes and peroxidation byproducts. From this study, it is concluded that BPS‐caused toxic effects in fish species lead to an imbalance in the antioxidant defense system. It is clearly indicated that BPS toxicity could lead to susceptible oxidative stress in various tissues and could damage vital organs.

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The effects of different bisphenol derivatives on oxidative stress, DNA damage and DNA repair in RWPE‐1 cells: A comparative study

Cited by 39 publications

References 73 publications

In vitro effects of bisphenol F on antioxidant system indicators in the isolated hepatocytes of rainbow trout (Oncorhyncus mykiss)

In vitro effects of bisphenol F on antioxidant system indicators in the isolated hepatocytes of rainbow trout (Oncorhyncus mykiss)

Bisphenol A and Bisphenol S Induce Endocrine and Chromosomal Alterations in Brown Trout

Biochemical responses and antioxidant defense mechanisms in Channa Striatus exposed to Bisphenol S

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