1998
DOI: 10.1002/elps.1150190816
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The effects of electroendosmosis in agarose electrophoresis

Abstract: The effects of agarose gel strips without and with 0.03 m(r) electroendosmosis (EEO) on isoelectric focusing (IEF) were studied. It is shown that only agarose without EEO can be used for IEF. The effects of electrode buffer strips using agarose with different EEO of 0, 0.03, 0.08, 0.20 m(r) on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and native PAGE were also studied. It apparently did not affect SDS-PAGE, but affected native PAGE to a certain extent. The higher the EEO value was, t… Show more

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Cited by 22 publications
(19 citation statements)
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“…The basic indicators for evaluating agarose are sulfate content, gel strength, EEO, gelling temperature, and melting temperature, and these indicators determine the quality of agarose [ 8 , 10 , 33 ]. The sulfate content, gel strength, and EEO of raw agar, desulfated agar, and commercial agarose are shown in Table 3 .…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…The basic indicators for evaluating agarose are sulfate content, gel strength, EEO, gelling temperature, and melting temperature, and these indicators determine the quality of agarose [ 8 , 10 , 33 ]. The sulfate content, gel strength, and EEO of raw agar, desulfated agar, and commercial agarose are shown in Table 3 .…”
Section: Resultsmentioning
confidence: 99%
“…EEO is one of the quality standards of agarose, and it refers to the movement of liquid in gel [ 2 , 33 ]. The anionic group in agarose gel is fixed on the matrix and cannot move, but it can prevent the decomposable counter cation from migrating to the cathode in the matrix.…”
Section: Resultsmentioning
confidence: 99%
“…8). Agarose gel fibres are negatively charged ( Z f = −40 charge per fibre), which creates an ion cloud surrounding fibres similar to the ion cloud surrounding the NPs to form the so-called electrostatic double layer (EDL) 48,49 .…”
Section: Resultsmentioning
confidence: 99%
“…The acid groups associate with hydrated positively charged counter-ions from the buffer system to create a double layer [20][21][22]. On application of an electric field, these ions migrate through the matrix towards the cathode (negative electrode) drawing water with them as part of the hydration sphere [19,[23][24][25]. This causes the net flow of solvent towards either the cathode or the anode depending on the membrane charge.…”
Section: Eeomentioning
confidence: 99%
“…The EEO phenomenon is used as the driving force in capillary electrophoresis [18]. In relation to gel and preparative membrane electrophoresis it denotes the bulk fluid flow through the gels or membranes caused by the presence of charges on the network [19]. While deliberate inclusion of charged groups to obtain EEO properties is desirable for capillary electrophoresis, for situations such as gel and preparative membrane electrophoresis EEO is often a problem encountered during electrophoretic separations.…”
Section: Eeomentioning
confidence: 99%