The effects of fermented rye products on gut microbiota and their association with metabolic factors in Chinese adults – an explorative study

Liu, Yuwei; Xue, Kun; Iversen, Kurt; Qu, Zheng; Dong, Chenglin; Jin, Taiyi; Hallmans, Göran; Åman, Per; Johansson, Anders; He, Gengsheng; Landberg, Rikard

doi:10.1039/d1fo01423d

Cited by 14 publications

(6 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The relative abundance of Collinsella , a microorganism associated with the risk of fatty liver disease ( Astbury et al, 2020 ), was also found in the same pattern. Romboutsia is also an important microorganism related to the production of acetic acid ( Liu et al, 2021 ) and butyric acid ( Qin et al, 2021 ). At the species level, the different treatments significantly altered the relative abundances of Romboutsia ilealis .…”

Section: Resultsmentioning

confidence: 99%

Naoxintong Capsule Alternates Gut Microbiota and Prevents Hyperlipidemia in High-Fat-Diet Fed Rats

Wan

et al. 2022

Front. Pharmacol.

View full text Add to dashboard Cite

Background: Naoxintong Capsule (NXT) is a formulated Traditional Chinese Medicine (TCM) widely applied in the treatment of cardiovascular and metabolic diseases, most of which are closely related to hyperlipidemia as a major risk factor. Given the current limited understandings to the role of gut microbiota in the lipid-lowering effect of NXT and other TCM products, this study investigated the regulation of gut microbiota and lipid metabolism by NXT, and their potential relationship.Methods: The chemical components of NXT were firstly analyzed with HPLC-MS method. In high fat diet (HFD)-fed rat models, as well as normal rats as control, the histopathological and biochemical changes of serum and liver were examined, including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C). In addition, the gut microbiota community was analyzed using 16S rRNA sequencing technique, the fecal levels of gut microbiota related metabolites, including bile acids (BAs) and short chain fatty acids (SCFAs) were determined with HPLC-MS. The correlations of the clinical indicators and gut microbiota related indicators were then investigated statistically.Results: The results showed that NXT exerted potential preventive effect on hyperlipidemia. Specifically, NXT significantly reduced the body weight, TC, TG and LDL-C in serum, increased HDL-C in serum, reduced the TC and TG in liver, as well as protected liver. The body weight, serum lipid levels and liver function were all significantly alleviated. The gut microbiota of the HFD-fed rats was reconstituted with supplementation of NXT. The fecal levels of gut microbiota related metabolites, including BAs and SCFAs were also altered. The correlation between the gut microbiota and clinical/metabolomic parameters was then studied. As the result, the amount of propionic aicd, Firmicutes/Bacteroidetes ratio (F/B) and the relative abundance of Collinsella in feces are the most possibly potential therapeutic biomarkers of NXT.Conclusion: NXT was effective in regulation of gut microbiota and prevention of hyperlipidemia in HFD fed rats. The present work might provide novel insights into the anti-hyperlipidemia effect of TCM and afford new scientific evidence for clinical application of TCM.

show abstract

Section: Resultsmentioning

confidence: 99%

Naoxintong Capsule Alternates Gut Microbiota and Prevents Hyperlipidemia in High-Fat-Diet Fed Rats

Wan

et al. 2022

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

“…IVA is a short‐chain fatty acid that can be found in human serum, feces, urine, and dental plaque. In clinical studies, altered concentrations of IVA are often detected in gastrointestinal disorders (Liu et al, 2021). Multiple analyses of H. pylori infection and irritable bowel syndrome have found an association (El‐Badry et al, 2018; Kim et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Targeted metabolomics analysis for serum Helicobacter pylori–positive based on liquid chromatography–tandem mass spectrometry

Wang

Shan

Zhang

et al. 2023

Biomedical Chromatography

View full text Add to dashboard Cite

Helicobacter pylori (H. pylori), as a harmful bacteria associated with gastric cancer, can have adverse effects on human normal flora and metabolism. However, the effects of H. pylori on human metabolism have not been fully elucidated. The 13C breathing test was used as the basis for distinguishing negative and positive groups. Serum samples were collected from the two groups for targeted quantitative metabolomics detection; multidimensional statistics were used, including partial least squares discriminant analysis (PLS‐DA), principal component analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS‐DA), and differential metabolites were screened. Unidimensional statistics combined with multidimensional statistics were used to further screen potential biomarkers, and finally pathway analysis was performed. SPSS 21.0 software package was used for statistical analysis of experimental data. Multivariate statistical analysis such as PLS‐DA, PCA, and OPLS‐DA was performed using Simca‐P 13.0 to search for differential metabolites. This study confirmed that H. pylori caused significant changes in human metabolism. In this experiment, 211 metabolites were detected in the serum of the two groups. Multivariate statistical analysis showed that PCA of metabolites was not significantly different between the two groups. PLS−DA indicated that the serum of the two groups was well clustered. There were significant differences in metabolites between OPLS−DA groups. By setting the variable importance in projection (VIP) threshold as one and the corresponding P−value <0.05, a total of 40 metabolites were screened in this study. P <0.05 and ∣log2FC∣>0 (where FC is the fold change) were used together as a unidimensional statistical filter condition. The analysis found that the expression of 15 metabolites such as propionic acid, acetic acid, adipic acid increased, and the metabolism of six products such as deoxycholic acid (DCA), 4−hydroxyphenylpyruvic acid, pyruvic acid decreased. P <0.05, false discovery rate <0.5, ∣log2FC∣>1, and OPLSDA_VIP>1 were used together as a condition for filter screening potential biomarkers. Four potential biomarkers were screened, which were sebacic acid, isovaleric acid, DCA, and indole−3−carboxylic acid. Finally, the different metabolites were added to the pathway‐associated metabolite sets (SMPDB) library for the corresponding pathway enrichment analysis. The significant abnormal metabolic pathways were taurine and subtaurine metabolism, tyrosine metabolism, glycolysis or gluconeogenesis, pyruvate metabolism, etc. This study shows that H. pylori has an impact on human metabolism. Not only a variety of metabolites have significant changes, but also metabolic pathways are abnormal, which may be the reason for the high risk of H. pylori causing gastric cancer.

show abstract

“…28,29 Clostridium sensu stricto 1, Romboutsia, Ruminococcaceae UCG 013, and the Eubacterium ventriosum group, which have been reported to be efficient fermenters capable of producing SCFAs were found in abundance after L. rhamnosus and L-LXOS intervention. [30][31][32][33] The effects of L-HXOS on gut microbiota were associated with significantly increasing levels of Bifidobacterium, Ruminiclostridium 9, Bacteroides, GCA 900066575, and Tyzzerella. Consistent with our findings, a large number of in vivo and in vitro studies consistently showed significant bifidogenic changes in the gut microbiota under XOS stimulation.…”

Section: Discussionmentioning

confidence: 99%

Dose-dependent effects of xylooligosaccharides on glycemic regulation with L. rhamnosus CCFM1060 in diabetic mice

Cui

Liu

et al. 2023

Food Funct.

View full text Add to dashboard Cite

show abstract

The effects of fermented rye products on gut microbiota and their association with metabolic factors in Chinese adults – an explorative study

Abstract: Rye is among the cereals with highest content of dietary fibre. A high rye food intake has been associated with improved metabolic risk factors in some but not all observational...

Cited by 14 publications

References 29 publications

Naoxintong Capsule Alternates Gut Microbiota and Prevents Hyperlipidemia in High-Fat-Diet Fed Rats

Naoxintong Capsule Alternates Gut Microbiota and Prevents Hyperlipidemia in High-Fat-Diet Fed Rats

Targeted metabolomics analysis for serum Helicobacter pylori–positive based on liquid chromatography–tandem mass spectrometry

Dose-dependent effects of xylooligosaccharides on glycemic regulation with L. rhamnosus CCFM1060 in diabetic mice

Contact Info

Product

Resources

About