The effects of follicular fluid on in vitro maturation, oocyte fertilization and the development of bovine embryos

Kim, K S; Mitsumizo, Naruki; Fujita, Kaden K.; Utsumi, Kyozo

doi:10.1016/0093-691x(96)00008-8

Cited by 46 publications

(27 citation statements)

References 33 publications

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“…The present study indicated that heparin affinity chromatography is an effective method to derive active factor(s) in bFF for promoting developmental competence of bovine oocytes. Kim et al [30] previously reported that supplementation of 10-30%(v/v) pooled bFF into IVM medium had no effect on developmental rates to the blastocyst stage, similarly to the present results (Fig. 2), whereas addition of 60% bFF had inhibitory effects.…”

Section: Discussionsupporting

confidence: 79%

In Vitro Maturation of Bovine Oocytes with Fractions of Bovine Follicular Fluid Separated by Heparin Affinity Chromatography.

Ikeda

Azuma

Hashimoto

et al. 1999

Journal of Reproduction and Development

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Abstract.To elucidate the beneficial effects of follicular fluid (FF) added to maturation medium on nuclear maturation and postfertilization development of bovine oocytes in vitro, we attempted to separate largely bovine FF (bFF) collected from small follicles into heparin-binding and -nonbinding fractions (HBF and HNF, respectively) by heparin affinity chromatography. Each fraction was dialyzed (molecular weight cut off 1,000 Da) and lyophilized, then dissolved in a half original bFF volume of modified synthetic oviduct fluid (mSOF). Experiment 1: Cumulus-oocyte-complexes (COCs) were matured in mSOF in the presence or absence (control) of whole bFF (wFF), HBF or HNF at a concentration of 10% (v/v). After in vitro fertilization, oocytes were freed from COCs and then cultured in mSOF with fetal calf serum until Day 8. Nuclear maturation rates were not different among wFF, HBF, HNF and control (69.8, 75.6, 63.6 and 72.9%, respectively). However, HBF favored postfertilization development, especially to the blastocyst stage (27.2%) compared with the control (19.0%), whereas HNF showed inhibitory effects, although wFF had no effect. Experiment 2: To determine whether the effects of HBF and HNF would become more evident by high-dose supplementation, HBF or HNF was added to in vitro maturation (IVM) medium at concentrations of 10,20 or 40% (v/v). HBF at all concentrations examined resulted in significantly higher (p<0.05) cleavage rates (80.5-86.2%) and blastocyst yield (31.1-44.2%) than the control(cleavage: 70.1%, blastocyst: 22.4%), whereas high dosages of HNF ( 20%) markedly inhibited embryonic development. These results indicated that heparin-binding fraction of bFF when added to the IVM medium was highly effective for enhancement of developmental competence of bovine oocytes in vitro.

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Section: Discussionsupporting

confidence: 79%

In Vitro Maturation of Bovine Oocytes with Fractions of Bovine Follicular Fluid Separated by Heparin Affinity Chromatography.

Ikeda

Azuma

Hashimoto

et al. 1999

Journal of Reproduction and Development

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“…Furthermore, when horse oocytes are matured in vitro in a medium supplemented with 250 ng/ml progesterone, the cumulus cells undergo a greater degree of expansion (Schmid et al, 1998). Kim et al (1996) showed that the use of follicular fluid in proportions of 10-30% facilitated nuclear and cytoplasmic maturation, which is in conformity with our results. Nevertheless, Aguilar et al (2001) obtained good maturation results using high percentages (up to 50%) of follicular fluid.…”

Section: Tablesupporting

confidence: 82%

Effect of thein vitromaturation medium on equine oocytes: Comparison of follicular fluid and oestrous mare serum

Gil

Saura²,

Echegaray³

et al. 2005

Acta Veterinaria Hungarica

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The present study evaluated the effect of supplementing the medium used to mature equine oocytes in vitro with oestrous mare serum (EMS) or horse follicular fluid (HFF). To this end, 144 ovaries were obtained from mares aged 16-21 months and transported to the laboratory in Dulbecco's phosphate buffered saline (D-PBS) at 30 °C. Oocytes were harvested from the ovaries by slicing, and then selected for in vitro maturation (IVM) according to the number of cumulus cell layers and the characteristics of the cytoplasm. The selected oocytes were washed three times in TCM199 medium plus HEPES (TCM-199H) or in the same medium plus glutamine (TCM-199G), then matured in vitro in six study groups established according to the in vitro maturation (IVM) treatment to see possible interactions between HEPES and glutamine on other supplements: Ten percent EMS was added to two of these media and 10% HFF was added to the media in two other groups . IVM was performed at 38.5 °C for 40 h in a controlled atmosphere (5% CO 2 , 95% relative humidity). The findings indicate that the presence of EMS or HFF in the TCM-199H medium gives rise to the best results in terms of the proportions of oocytes reaching maturity (37.7% and 36.8%, respectively). The values obtained with EMS and HFF were statistically similar to each other but differed from the other treatments. The media containing glutamine led to the highest proportions of degenerated oocytes.

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“…Não houve diferença (P>0,05) na taxa de maturação dos ovócitos de diferentes tamanhos de folículos, pois mais de 80% atingiram o estádio de MII em um período de 24 horas, em todos os grupos (Tabela 3). As taxas de maturação obtidas são semelhantes às citadas na literatura quanto a B. taurus (Kim et al, 1996;Gandolfi et al, 1997). Entretanto, quando o tamanho dos folículos foi considerado, Pavlok et al (1992) e Fair et al (1995) mostraram que em ovócitos provenientes de folículos menores do que 2 mm a taxa de maturação nuclear é menor do que em ovócitos de folículos acima de 3 mm.…”

Section: Resultsunclassified

Efeito do tamanho do folículo na maturação nuclear e citoplasmática de ovócitos de fêmeas zebuínas

Dode

Rodovalho

Ueno

et al. 2000

Pesq. agropec. bras.

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RESUMO -Este estudo avaliou o efeito do tamanho do folículo na capacidade dos ovócitos de sofrerem maturação nuclear e citoplasmática. Ovários de vacas Nelore (Bos indicus) foram coletados logo após o abate, e os complexos cúmulus-ovócitos foram aspirados de folículos de diferentes categorias, e classificados de acordo com seus diâmetros em: 1-2 mm, 3-5 mm, 6-8 mm e ≥9 mm. A medida do diâmetro e a fixação dos ovócitos para avaliação do estádio nuclear antes da maturação foram feitas logo após a aspiração. Ovócitos morfologicamente viáveis foram maturados, fecundados e cultivados in vitro. Os ovócitos obtidos de folículos de 1-2 mm apresentaram menor diâmetro (P<0,01) do que os dos demais grupos. Antes da maturação, 89,8%, 90,1%, 85,7% e 100% dos ovócitos provenientes de folículos de 1-2, 3-5, 6-8 e ≥9 mm de diâmetro, respectivamente, apresentavam vesícula germinativa. O tamanho do folículo não influenciou (P<0,05) a taxa de maturação nuclear. A porcentagem de ovócitos que chegaram a metáfase II foi de 88,8%, 87,8%, 92,9% e 100% no que se refere aos ovócitos de folículos de 1-2, 3-5, 6-8 e ≥9 mm, respectivamente. As taxas de penetração e clivagem foram semelhantes (P>0,05) nos grupos de 1-2 mm (93,4% e 81,9%), 3-5 mm (90,7% e 79,6%), 6-8 mm (91,3% e 77,8%) e ≥9 mm (92,0% e 78,3%). Da mesma forma, não houve diferenças (P>0,05) entre as categorias de folículos no que se refere às taxas de polispermia, descondensação da cabeça do espermatozóide e formação de pronúcleos. Os resultados deste estudo demonstram que na espécie B. indicus, folículos com diâmetro de 1 mm até ≥9 mm não influenciaram a capacidade dos ovócitos de reiniciar e completar a meiose e de clivar após maturação e fecundação in vitro.Termos para indexação: bovino, diâmetro folicular, competência meiótica, Nelore, Bos indicus. EFFECT OF FOLLICLE SIZE IN NUCLEAR AND CYTOPLASMIC MATURATION OF OOCYTES FROM ZEBU COWSABSTRACT -The objective of this study was to investigate the effect of follicle size on the ability of the oocytes to undergo nuclear and cytoplasmic maturation. Ovaries from Nelore cows (Bos indicus) were collected just after slaughter. The cumulus oocyte complexes were aspirated from four size categories follicles: 1-2 mm, 3-5 mm, 6-8 mm and ≥9 mm of diameter. The oocytes were measured and evaluated for chromatin configuration before maturation. After selection the oocytes were matured, fertilized and cultured in vitro. Oocytes obtained from follicles of 1-2 mm showed a smaller diameter (P<0.01) than the other follicle groups. Before maturation 89.8%, 90.1%, 85.7% and 100% of oocytes from follicles of 1-2 mm, 3-5 mm, 6-8 mm and >9 mm, respectively, showed a germinal vesicle. A similar maturation rate (P<0.05) was observed for all groups, with 88.8%, 87.8%, 92.9% and 100.0% of the oocytes in metaphase II for follicles of 1-2, 3-5, 6-8 and >9 mm, respectively. The penetration and clivage rates were also similar (P>0.05) for oocytes obtained from follicle of 1-2 mm (93.4% and 81.9%), 3-5 mm (90.7% and 79.6%), 6-8 mm (91.3% and 77.8%) and ≥9 mm (...

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The effects of follicular fluid on in vitro maturation, oocyte fertilization and the development of bovine embryos

Cited by 46 publications

References 33 publications

In Vitro Maturation of Bovine Oocytes with Fractions of Bovine Follicular Fluid Separated by Heparin Affinity Chromatography.

In Vitro Maturation of Bovine Oocytes with Fractions of Bovine Follicular Fluid Separated by Heparin Affinity Chromatography.

Effect of thein vitromaturation medium on equine oocytes: Comparison of follicular fluid and oestrous mare serum

Efeito do tamanho do folículo na maturação nuclear e citoplasmática de ovócitos de fêmeas zebuínas

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