Background: Lower extremity stiffness simulates the response of the lower extremity to landing in running. However, its relationship with running economy (RE) remains unclear. This study aims to explore the relationship between lower extremity stiffness and RE.Methods: This study utilized articles from the Web of Science, PubMed, and Scopus discussing the relationships between RE and indicators of lower extremity stiffness, namely vertical stiffness, leg stiffness, and joint stiffness. Methodological quality was assessed using the Joanna Australian Centre for Evidence-Based Care (JBI). Pearson correlation coefficients were utilized to summarize effect sizes, and meta-regression analysis was used to assess the extent of this association between speed and participant level.Result: In total, thirteen studies involving 272 runners met the inclusion criteria and were included in this review. The quality of the thirteen studies ranged from moderate to high. The meta-analysis results showed a negative correlation between vertical stiffness (r = −0.520, 95% CI, −0.635 to −0.384, p < 0.001) and leg stiffness (r = −0.568, 95% CI, −0.723 to −0.357, p < 0.001) and RE. Additional, there was a small negative correlation between knee stiffness and RE (r = −0.290, 95% CI, −0.508 to −0.037, p = 0.025). Meta-regression results showed that the extent to which leg stiffness was negatively correlated with RE was influenced by speed (coefficient = −0.409, p = 0.020, r2 = 0.79) and participant maximal oxygen uptake (coefficient = −0.068, p = 0.010, r2 = 0.92).Conclusion: The results of this study suggest that vertical, leg and knee stiffness were negatively correlated with RE. In addition, maximum oxygen uptake and speed will determine whether the runner can take full advantage of leg stiffness to minimize energy expenditure.