The Effects of Gamma Irradiation on the Growth and Propagation of &lt;em&gt;In-Vitro&lt;/em&gt; Chrysanthemum Shoot Explants (cv. &lt;em&gt;Yellow Puma&lt;/em&gt;)

Dwimahyani, Ita; Widiarsih, Sasanti

doi:10.17146/aij.2010.25

Cited by 17 publications

(7 citation statements)

References 4 publications

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“…Gamma irradiation inhibited in vitro shoot elongation of patchouli. Effect of gamma irradiation on inhibition of shoot length was observed in Triticum aestivum L. [29] Cicer [30], Corn [31], and Chrysanthemum [32]. Gamma irradiation prevented the shoot elongation of Cicer.…”

Section: Resultsmentioning

confidence: 99%

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The Effect of Gamma Irradiation on the Growth and Multiplication of the In Vitro Shoot of Patchouli (Pogostemon cablin Benth.)

Banyo

Indriyani

Widoretno

2020

JELS

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The objective of this research was to evaluate the effect of gamma irradiation on shoot growth and multiplication of Patchouli (Pogostemon cablin Benth.) Two weeks-old in vitro shoots were irradiated gamma-ray, at doses of 0, 15, 30, 45, 60, and 75 Gy. The control shoot was not irradiated. The irradiated shoots were cultured on Murashige and Skoog (MS) medium supplemented with 0.1 mg.L-1 NAA and 0.3 mg.L-1 BA and incubated in a growth room for eight weeks at a temperature of 25±2°C. The results showed that the gamma irradiation inhibited the growth and multiplication of shoots. Explants irradiated with high-dose gamma-ray (45-75 Gy) had not formed shoot in four weeks of culture, while 58.3-83.3% of the explants without irradiation or irradiated at low doses 15 and 30 Gy formed shoots. The higher irradiation doses increased percentage of browning explants and reduced the percentage of forming shoots. Within the eight weeks of culture, explant without irradiation was able to form shoots at the percentage of 100% with 24 shoots per explant, while explants irradiated at 15-45 Gy were able to grow form shoots at the percentage of 77.7-95.5%. The high doses-irradiated explants (60 and 75 Gy) were only able to form shoots less than 13-20%, with 2-3 shoots per explant.

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Section: Resultsmentioning

confidence: 99%

“…The higher irradiation, shoot length was more affected [30]. Previous research found that gamma irradiation decreased the number of leaves and branches on Chrysanthemum [32]. Gamma-ray irradiation also had a significant impact on the shoot length of Triticum aestivum L. The shoot length decreased by 46% as the gamma dose increased [29].…”

Section: Resultsmentioning

confidence: 99%

The Effect of Gamma Irradiation on the Growth and Multiplication of the In Vitro Shoot of Patchouli (Pogostemon cablin Benth.)

Banyo

Indriyani

Widoretno

2020

JELS

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“…Increasing demand for the new form of chrysanthemum leads to research for obtaining new varieties. Mutation breeding by radiation, a farming appliance of nuclear technology, and has been widely exploited to develop welladapted plant varieties by one or a few important qualities (Kumar et al 2006, Jain and Spencer (2006, Dwimahyani and Widiarsih 2010) Commercially important traits in horticulture plants have been altered positively by the various physical mutagens. Gamma rays are widely used among the physical mutagens for inducing mutations in owering plants due to their easy application and high e ciency.…”

Section: Introductionmentioning

confidence: 99%

Congenial in Vitro γ-Ray Induced Mutagenesis Underlying the Varied Array of Petal Colours in Chrysanthemum (Dendranthemum Grandiflorum Kitam). ‘Candid’

Din¹,

Qadri²,

Wani³

et al. 2021

Preprint

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Chrysanthemum (Dendranthemum grandiflorum kitam.) is amongst the top ten cut flowers globally. The flower colour of ancestral species is restricted to white, yellow, and pink and is acquired from anthocyanins, carotenoids, and the dearth of both pigments, respectively. An extensive array of flower colours, like orange, dark red, purplish-red, and red, has been bred by enhancing the variety of pigments or the amalgamation of both pigments. In recent times, green-flowered cultivars having chlorophylls in their ray petals have been produced and have grown a reputation. Furthermore, violet /blue flowers have been bred via transgenic interventions. Flower colour is considered as critically acclaimed feature of any flower cultivar especially chrysanthemum. Creating newer chrysanthemum cultivars with novel features, for instance, new flower colours in a time and input optimised approach, is the eventual ambition for breeders. Exploring the molecular mechanisms that control flower pigmentation may present imperative suggestions for the rational manoeuvring of flower colour. To generate a diverse array of flower colour mutants in chrysanthemum cv. “Candid” through mutagenesis, in vitro grown micro shoots were exposed to 10, 20, 30, and 40 Gy gamma irradiation at 100 Gy per minute and were evaluated for different parameters. The rhizogenesis parameters declined with the increase in irradiation dose from 0 Gy to 40 Gy, while as, 10 Gy dose proved to record minimum decline in contrast to the control. Survival, leaf size, and the number of leaves plant− 1 after the 8th -week interval also decreased with the increasing trend of gamma irradiation dose but recorded a minimum decline in plants raised from shoots irradiated with 10 Gy gamma irradiation dose with respect to the control. Apparently, the minimum delay in the number of days to floral bud appearance took under 10 Gy compared to control. The highest number of flower colour mutants were recorded under 10 Gy (light pink, orange-pink, white and yellow). Demountable mutation frequency based on flower colour was desirable in plants irradiated with the slightest dose of 10 Gy

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“…To create novel Chrysanthemum ower color/shape mutants, radiation has been used successfully (Misra et al 2003;Dwimahyani and Widiarsih 2011). As a result, radiation-induced mutagenesis has become a critical approach for ower breeding.…”

Section: Introductionmentioning

confidence: 99%

Mechanisms generating the numerous hues of chrysanthemum petals can be traced to in vitro γ-ray-induced mutagenesis.

Din

Qadri

Wani

et al. 2021

Preprint

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In terms of global cut flower production, chrysanthemum (Dendranthemum grandiflorum kitam.) ranks among the top ten. When a plant species possesses colour characteristics associated with those of ancestral species, that colour is described as being white, yellow, or pink, with white coloration representing an absence of pigments, and yellow and pink coloration each represents the presence of carotenoids and anthocyanins, respectively. Pigments, or a combination of pigments, have been improved upon to provide an expansive rainbow of floral colours like orange, dark red, purplish-red, and red. Recent green-flowered varieties have developed a reputation for containing chlorophylls in their ray petals. Additionally, transgenic interventions have been used to breed violet/blue flowers. Flower colour is a highly regarded characteristic of any flower cultivar, particularly chrysanthemum. Breeders' ultimate goal is to create newer chrysanthemum cultivars with innovative characteristics, such as new flower colours, using a time and input-efficient approach. Investigating the molecular mechanisms that regulate flower pigmentation may yield critical insights into the rational manipulation of floral colour. To generate a diverse array of flower colour mutants in chrysanthemum cv. “Candid” through mutagenesis, in vitro grown micro shoots were exposed to 10, 20, 30, and 40 Gy gamma irradiation at 100 Gy per minute and were evaluated for different parameters. The rhizogenesis parameters declined with the increase in irradiation dose from 0 Gy to 40 Gy, while as, 10 Gy dose proved to record minimum decline in contrast to the control. Survival, leaf size, and the number of leaves plant-1 after the 8th-week interval exhibited a downward trend with the increasing trend of gamma irradiation dose but recorded a least drop in plants raised from shoots irradiated with 10 Gy gamma irradiation dose with respect to the control. Apparently, the minimum delay in the number of days to floral bud appearance took under 10 Gy compared to control. The highest number of flower colour mutants was recorded under 10 Gy (light pink, orange-pink, white and yellow). Demountable mutation frequency based on flower colour was desirable in plants irradiated with the slightest dose of 10 Gy.

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The Effects of Gamma Irradiation on the Growth and Propagation of <em>In-Vitro</em> Chrysanthemum Shoot Explants (cv. <em>Yellow Puma</em>)

Cited by 17 publications

References 4 publications

The Effect of Gamma Irradiation on the Growth and Multiplication of the In Vitro Shoot of Patchouli (Pogostemon cablin Benth.)

The Effect of Gamma Irradiation on the Growth and Multiplication of the In Vitro Shoot of Patchouli (Pogostemon cablin Benth.)

Congenial in Vitro γ-Ray Induced Mutagenesis Underlying the Varied Array of Petal Colours in Chrysanthemum (Dendranthemum Grandiflorum Kitam). ‘Candid’

Mechanisms generating the numerous hues of chrysanthemum petals can be traced to in vitro γ-ray-induced mutagenesis.

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