The Effects of Heat Treatment on the Gene Expression of Several Heat Shock Protein Genes in Two Cultivars of Strawberry

Brown, R. G.; Wang, Hehe; Dennis, Melanie; Slovin, Janet P.; Turechek, William W.

doi:10.1080/15538362.2016.1199996

Cited by 14 publications

(6 citation statements)

References 23 publications

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Section: Heat-stress Results In Loss Of Methylation In Regions Flanki...mentioning

confidence: 99%

“…However, little is known about how these responses are influenced directly by changes in DNA methylation or vice versa . Transcription factor families such as AP2/EREBP and HSFs play important roles in response to abiotic stresses in plants including strawberry, apple and maize (Brown et al , 2016; H. C. Liu et al , 2011; Qian et al , 2019; Xie et a l., 2019; C. L. Zhang et al , 2020). More over, some AP2/EREBP genes are known to be highly induced under heat stress conditions and up-regulated by HSFs through an interconnected stress regulatory network (Q.…”

Section: Discussionmentioning

confidence: 99%

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DNA methylation dynamics during stress-response in woodland strawberry (Fragaria vesca)

López

Roquis

Becker

et al. 2022

Preprint

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SummaryEnvironmental stresses can result in a wide range of physiological and molecular responses in plants. These responses can also impact epigenetic information in genomes especially at the level of DNA methylation. DNA methylation is the hallmark heritable epigenetic modification and plays a key role in silencing transposable elements (TEs). Although DNA methylation is an essential epigenetic mechanism, fundamental aspects of its contribution to stress responses and adaptation remain obscure.We investigated epigenome dynamics of wild strawberry (Fragaria vesca) in response to variable environmental conditions at DNA methylation level. F. vesca methylome responded with great plasticity to ecologically relevant abiotic and hormonal stresses. Thermal stress resulted in substantial genome-wide loss of DNA methylation. Notably, all tested stress conditions resulted in marked hot spots of differential DNA methylation near centromeric or pericentromeric regions, particularly in non-symmetrical DNA methylation context. Additionally, we identified differentially methylated regions (DMRs) within promoter regions of transcription factor (TF) superfamilies involved in plant stress-response and assessed the effects of these changes on gene expression.These findings improve our understanding on stress-response at the epigenome level by highlighting the correlation between DNA methylation, TEs and gene expression regulation in plants subjected to a broad range of environmental stresses.

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Section: Heat-stress Results In Loss Of Methylation In Regions Flanki...mentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

DNA methylation dynamics during stress-response in woodland strawberry (Fragaria vesca)

López

Roquis

Becker

et al. 2022

Preprint

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“…Thermotherapy, also known as ‘passive heating’ in medical science, has been proven to be beneficial in curing metabolic diseases in humans as the gradual heating process activates heat shock proteins, a protein family that is associated with the adaptation to heat stress ( Faulkner et al, 2017 ). The pre-treatment phase of thermotherapy in plants is similar to the passive heating in humans, and the process has been shown to activate heat shock proteins in the plants as well, thereby enabling plants to withstand gradual heat stress ( Gulen and Eris, 2015 ; Brown et al, 2016 ). A precise temperature control during the therapeutic phase facilitates the use of higher temperatures with less risk of injury to the plants.…”

Section: Discussionmentioning

confidence: 99%

“…For steam treatment, strawberry roots were separated from the soil, gently washed with water, and the plants were placed on autoclave standard plastic trays in a steam chamber that generated aerated steam. The treatment process included a pre-treatment phase of 37°C for 60 min ( Brown et al, 2016 ), followed by 60 min cool down at the then ambient temperature, and subsequent heat treatment at 44°C for 60, 120, or 240 min ( Wang et al, 2019 ). The steam-treated plants were replanted in new plastic pots containing 1 kg of sterilized soil in the greenhouse with ambient temperatures of 33 ± 5°C.…”

Section: Methodsmentioning

confidence: 99%

Steam-based thermotherapy for managing nematodes in strawberry transplants

Khanal

Peres

et al. 2020

Journal of Nematology

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Aerated steam-based thermotherapy was developed and evaluated for its efficacy in managing three nematode species (Aphelenchoides besseyi, Meloidogyne hapla, and Pratylenchus penetrans) that are often transported as quiescent passengers on strawberry transplants shipped to Florida from out-of-state nurseries. Initial studies were focused on evaluating the intrinsic temperature sensitivity of each nematode species to hot water in laboratory conditions. Each nematode species was exposed to hot water at 40, 44, 48, and 52°C for 1, 5, 10, 30, 60, 120, and 240 min. Exposure for 60 min or higher at 40°C paralyzed all three nematode species when examined immediately after heat treatment. Examination of the nematodes 24 hr post-treatment suggested that 100% mortality of all three nematode species was achieved when nematodes were exposed to hot water at a minimum temperature of 44°C for 120 min. Further studies were conducted to evaluate the efficacy of aerated steam to kill all three nematode species by exposing nematode-infested strawberry transplants at 44°C for 60, 120, and 240 min. Exposure of nematode inoculated plants to steam for 60 or 120 min reduced the populations of all three nematode species, but this was not enough to completely eradicate any of the three nematode species. Exposure for 240 min, however, was the most effective in reducing the populations of the three nematode species. A 240 min of exposure to aerated steam completely eradicated A. besseyi and M. hapla while P. penetrans populations were reduced only by 85%. Furthermore, the aerated steam had minimal to no adverse effect on plant biomass. Results from both the laboratory and greenhouse studies indicated that M. hapla was more sensitive to heat treatment followed by A. besseyi and P. penetrans. Results from this study suggested that aerated steam-based thermotherapy has good potential as a non-chemical method of management of nematodes of strawberry transplants.

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“…In other trials, non-dormant runner plants of seven cultivars all tolerated treatments killing endoparasitic nematodes (Staniland, 1953). Brown et al (2016) developed a technique for warm water dipping of strawberry transplants in Florida. It starts with a pre-conditioning treatment at 37 °C to induce heat shock proteins that increase heat tolerance in the strawberry plants.…”

Section: Introductionmentioning

confidence: 99%

Steam thermotherapy strongly reduces Botrytis in strawberry transplants with no or minor negative effects on plant growth and yield

Gahatraj

Nielsen

et al. 2023

Eur J Plant Pathol

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The effect of steam thermotherapy on Botrytis spp. populations in strawberry transplants was evaluated. Tray plants rooted in 0.2 L peat plugs of seasonal flowering cvs. Falco, Sonsation, and Soprano, and everbearing cvs. Favori and Murano were pre-treated with steam at 37 °C for 1 h, followed by 1 h at ambient temperature and air humidity, and then 2 or 4 h steam treatment at 44 °C. Except for one cultivar with a slight reduction in yield, there were no negative effects on plant performance. Compared to untreated transplants, mean incidence of Botrytis on the five cultivars was reduced by 43 and 86% with the 2 and 4 h treatments, respectively. Within cultivars the reduction was significant in 2 and 3 experiments following the 2 and 4 h treatments, respectively. Sclerotia from four different isolates of Botrytis were subjected to treatment including 4 h of steam thermotherapy and subsequently tested for viability. Following 14 days of incubation, 90 to 100% (mean 97%) of treated sclerotia failed to produce mycelial growth compared with untreated sclerotia, which all germinated and produced mycelia. Botrytis isolates recovered from both treated and untreated strawberry transplants were tested for resistance to seven fungicides, including boscalid, fenhexamid, fludioxonil, fluopyram, pyraclostrobin, pyrimethanil and thiophanate-methyl. Multiple fungicide resistance was common; 35.5% of isolates were resistant to fungicides from at least three FRAC groups. Results indicate that steam thermotherapy treatment strongly reduces populations of Botrytis spp., including fungicide-resistant strains, in strawberry transplants with negligible negative impacts on the transplants.

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The Effects of Heat Treatment on the Gene Expression of Several Heat Shock Protein Genes in Two Cultivars of Strawberry

Cited by 14 publications

References 23 publications

DNA methylation dynamics during stress-response in woodland strawberry (Fragaria vesca)

DNA methylation dynamics during stress-response in woodland strawberry (Fragaria vesca)

Steam-based thermotherapy for managing nematodes in strawberry transplants

Steam thermotherapy strongly reduces Botrytis in strawberry transplants with no or minor negative effects on plant growth and yield

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