The effects of heterologous antilymphocyte serum on the distribution of 54Crlabeled lymph node cells in mice

Rn, Taub; Lance, Eugene M.

doi:10.1097/00007890-196907000-00023

Cited by 6 publications

(8 citation statements)

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“…It has been shown by Cantor et al (28) that the majority of immunologically competent cells in the spleen are derived from the peripheral recirculating pool of lymphocytes. Hence our findings are consistent with the generalization (17,27,29) that the principal site of action of ALS is the peripheral recirculating lymphocyte.…”

Section: Effectsupporting

confidence: 92%

The Effect of Heterologous Anti-Lymphocyte Serum on Lymphocytes of Thymus and Marrow Origin

Jeejeebhoy

1970

The Journal of Experimental Medicine

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When CY-treated mice were given sheep red blood cells the serum hemagglutinin titers produced were significantly lower than those found when mice received SRBC but not CY. Titers could be raised to the levels found in the latter group if, in addition to SRBC, the CY-treated mice received 2 x 107 normal syngeneic spleen cells or a mixture containing 1.2 x 108 normal thymus and 1.2 x 108 normal marrow cells. Inocula which contained fewer cells produced correspondingly smaller amounts of antibody. A synergistic interaction between normal thymus and marrow cells was always demonstrable in these experiments. Hemagglutinin titers produced by CY-treated mice given SRBC and 2 x 107 normal syngeneic spleen cells were always much higher than those found when the spleen cells were obtained from animals previously given ALS. Titers could be raised to normal levels if the animals in this latter group received additional injections containing mixtures of normal syngeneic thymus and marrow cells. Marrow cells alone were completely ineffective, while inocula which only contained thymus cells were much less effective than mixtures of thymus and marrow cells. These results suggest that immunosuppression by ALS is associated with the inactivation of both thymus and marrow-derived lymphocytes. In other experiments CY-treated mice received SRBC and mixtures of thymus and marrow cells from both untreated and ALS-treated donors. No decrease in the immunological competence of cells located within the thymus of ALS-treated donors was demonstrable in these experiments. Marrow cells were slightly affected but to a markedly lesser degree than were spleen cells of ALS-treated animals. In a final experiment, it was possible to show that the thymus and marrow cells of ALS-treated animals could repair the immunological defects which were present in their own spleen cell populations.

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Section: Effectsupporting

confidence: 92%

The Effect of Heterologous Anti-Lymphocyte Serum on Lymphocytes of Thymus and Marrow Origin

Jeejeebhoy

1970

The Journal of Experimental Medicine

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“…When density gradient-separated *^'Crlabelled lymph node cells are injected into syngeneic rats, a standard set of lymph nodes (about % of those available) harvested 24 h later, contains on average 25% of the total radioactivity injected, while liver radioactivity accounts for about 20% (McNeiiage&Heslop 1980). While it is generally agreed that radioactivity associated with dead or damaged cells is found in the liver , Taub & Lance 1968, Zatz & Lance 1970. Gillette & Lance 197K Heslop & Hardy 1971, Gilletteetal.…”

Section: Criteria Of Death Of Intravenously Injected *'Cr-labelled Lymentioning

confidence: 99%

Natural Cytotoxicity: Early Killing of Allogeneic Lymphocytes in Rats

Heslop

Neilage

1983

Immunological Reviews

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In many strain combinations among inbred rats, intravenously injected 51Cr-labelled lymphocytes are destroyed in substantial numbers by unsensitized allogeneic hosts. Destruction of cells (referred to as natural cytotoxicity (NC)) occurs within a few hours of injection, and is characterised by a decreased accumulation of radioactivity in the lymph nodes and increased renal excretion of label by allogeneic hosts, as compared with the distribution of label in syngeneic recipients of the same cell suspension. An intact spleen is necessary for killing. The level of NC expressed is consistent for a given donor-host combination. Using arbitrary criteria to compare the levels of NC expressed by different donor-host combinations among inbred rats, 13 of 95 strain combinations have been shown to express high NC, 63 intermediate NC and 19 low NC. The level of NC expressed cannot be correlated with the extent to which donor and host differ in respect of known MHC genes. Segregation analysis has shown high NC to be controlled by at least 2 independently segregating genes, one of which is MHC-linked. It is possible to weaken or abrogate NC by the neonatal injection of bone-marrow cells from the donor strain, and to reverse this reduced reactivity by the injection of host strain lymphocytes. The substitution of either the donor (P1) or the host (P2) by the P1 X P2)F1 hybrid reduces or eliminates NC in strain combinations normally expressing high NC. It is currently uncertain whether NC can be augmented. In the single strain combination in which maturation has been studied, NC becomes evident during the 4th week of life and attains adult levels during the 6th-7th weeks. NC is at least partially radio-sensitive. Two groups of reactivities appear to be related to NC: (i) those which have been identified in the context of aberrant lymphocyte homing, and for which allogeneic lymphocytes are the targets; (ii) the group of natural resistance systems which includes NK cells, and whose reactivity is directed against a variety of other target cells.

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“…to divert injected lymphoid cells from the l\iiiphoid tissues and promote tlicir uptake by tlie liver was first reported by Martin and Miller (1967) using thoracic duct cells. Taiib and Lance (1968) obtained a similar result using lymph node cells pre-incubated with higii concentrations of R.A.M.L.S.. and attributed it to action of cytotoxic anti!j(Kly. However, Martin and Miller (1969) subsequently reported this phenomenon in detail, antl postulated that two processes were involved in the localization of the injected cells, one occuring when the cells were seiisiti/cd with high concentrations of R.A.M.L.S.…”

Section: The Activity Of Cytophilic Antibody In the Localization Of Imentioning

confidence: 65%

The Mechanism of Action of Heterologous Antilymphocytic Serum: The Biological Activity of Rabbit Macrophage‐cytophilic Antilymphocytic Antibody in Mice

Gill

1970

Aust J Exp Biol Med

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Summary. The existence and predonifnantly 7S nature of nmcrophage-t y tophi lie antiiwHly in r.ihbit anti-monsc lyinphocytt; st'rum has hcon tonfirnicd. Al>S()rption of rabl>il anti-iiiousc lymphocyte serum with liotnologous cflls reduced the titre of cytophilic activity deteniiined hy both direct and indirect methods, while most of the cytotoxic and agyhitinating activity was retained. Thi.s suKK"St'' the antibodies responsihlo for cytophilia exist as a separate claH.-i in rabl)it nnH-rnniise lymphocyte serum. Cvtophilif antibody also proinoteil the iittachincnt of thymus cells to Bidb/c matrophages both in vitro and in vivo, thi? attiulimcnt in the latter instance occurring only when the tliyinot-ytes were prc-iiuubatrd willi low antibody concentrations. Absorption nf rabbit anti-moiise lymphocyte sfruiii with rabbit tflts also rediic-ed ibJ ability in increase the localisation in the liver of injecti'd thynuicytes. while eluates of cytophilic antibody could promote significant uptake of these c-ells by the liver wheu incubated with them in vitro.The results generally confirm the opsonizing properties of rabbit anti-mouse lympliotyte .serum nnd indicate that rabbit cytophilic antibody is biolDgicMlly iiclivp ill mice.INTRODUCTION.

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The effects of heterologous antilymphocyte serum on the distribution of 54Crlabeled lymph node cells in mice

Cited by 6 publications

References 0 publications

The Effect of Heterologous Anti-Lymphocyte Serum on Lymphocytes of Thymus and Marrow Origin

The Effect of Heterologous Anti-Lymphocyte Serum on Lymphocytes of Thymus and Marrow Origin

Natural Cytotoxicity: Early Killing of Allogeneic Lymphocytes in Rats

The Mechanism of Action of Heterologous Antilymphocytic Serum: The Biological Activity of Rabbit Macrophage‐cytophilic Antilymphocytic Antibody in Mice

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