The Effects of Hydroxyethyl Starches on Intracellular Calcium in Platelets

Gamsjäger, Thomas; Gustorff, Burkhard; Kozek‐Langenecker, Sibylle A.

doi:10.1213/00000539-200210000-00013

Cited by 15 publications

(11 citation statements)

References 10 publications

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“…There was a second decrease of surface CD62 expression in viscous PAS by day 7, to a level lower ( P < 0·05) than PCs in plasma and this remained so to day 9. This was an unexpected phenomenon not observed in previous storage studies as the direct effects of HES on platelets exclude calcium flux, P‐selectin expression and detection, and intracellular activation processes either in vitro or in vivo [21,23].…”

Section: Discussionmentioning

confidence: 74%

“…1b). HES of varying molecular weights have been known to reduce the availability of glycoprotein (GP)IIbIIIa as a result of binding to platelet surfaces [11,21,22,24]. Possibly over the storage period, the relative inability to bind fibrinogen/fibrin due to HES and the lower availability of external fibrinogen/fibrin concentration in the viscous PAS could have fostered platelet quiescence.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of a novel viscous platelet additive solution and plasma: preparation and in vitro storage parameters of buffy‐coat‐derived platelet concentrates

et al. 2008

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Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Comparison of a novel viscous platelet additive solution and plasma: preparation and in vitro storage parameters of buffy‐coat‐derived platelet concentrates

et al. 2008

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“…This also indicates that both HES200/0.5 and HES130/0.4 solutions in vivo cause an overall and non‐selective impairment of platelet function by reducing the availability of functional receptor on the platelet membrane GP. HES does not inhibit platelets by interfering with calcium‐dependent intracellular signal transduction pathways (19). The pathophysiologic mechanism for the significantly decreased expression of activated GP complexes may be the direct non‐specific coating of platelets (20).…”

Section: Discussionmentioning

confidence: 99%

Effects of two different hydroxyethyl starch solutions (HES200/0.5 vs. HES130/0.4) on the expression of platelet membrane glycoprotein

Chen

Yan

et al. 2006

Acta Anaesthesiol Scand

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“…Subsequently, the technique was improved and redefined as "continuous" (29) or "real-time flow cytometry" (30,31). In the platelet research field, kinetic flow cytometry was at first applied to investigations of calcium kinetics fluxes (24,(32)(33)(34)(35)(36) or activation markers (35,37).…”

mentioning

confidence: 99%

Flow Cytometric Monitoring of Dynamic Cytosolic Calcium, Sodium, and Potassium Fluxes Following Platelet Activation

2020

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The interaction of platelet agonists with their respective membrane receptors triggers intracellular signaling, among which cytosolic ion fluxes play an important role in activation processes. While the key contribution of intercellular free calcium is accepted, sodium and potassium roles in platelet activation have been less investigated in recent studies. Here, we implemented a novel flow‐cytometric method to monitor over time cytosolic free calcium, sodium, and potassium ion fluxes upon platelet activation and we demonstrate the feasibility of real‐time visualization of ion kinetics, in particular with a focus on sodium and potassium. Platelets were loaded with selective ion indicators, Fluo‐3 (Ca2+), ION NaTRIUM Green‐2 (Na+), and ION Potassium Green‐2 (K+). Fluorescence was monitored by flow cytometry. After measurement of a stable baseline, platelets were activated and ion indicator fluorescence was acquired over time, up to 10 min. Platelets were activated with either thromboxane analogue U46619, ADP, thrombin, TRAP6 (PAR‐1 agonist), AYPGKF (PAR‐4 agonist), convulxin (collagen receptor GPVI agonist), or combinations thereof. We evaluated preanalytical parameters (in particular dye loading time and concentration) to implement an accurate method. Subsequently, we characterized cytosolic calcium, sodium, and potassium kinetics in response to platelet agonists. We observed different patterns of agonist synergism. In conclusion, the present work highlights the use of cytosolic ion monitoring by flow cytometry to investigate characteristic calcium, sodium, and potassium mobilization patterns following platelet activation. This easy technique opens a new way to analyze signaling in different platelet subpopulations and it should prove useful for investigating platelet pathophysiology. © 2020 International Society for Advancement of Cytometry

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The Effects of Hydroxyethyl Starches on Intracellular Calcium in Platelets

Cited by 15 publications

References 10 publications

Comparison of a novel viscous platelet additive solution and plasma: preparation and in vitro storage parameters of buffy‐coat‐derived platelet concentrates

Comparison of a novel viscous platelet additive solution and plasma: preparation and in vitro storage parameters of buffy‐coat‐derived platelet concentrates

Effects of two different hydroxyethyl starch solutions (HES200/0.5 vs. HES130/0.4) on the expression of platelet membrane glycoprotein

Flow Cytometric Monitoring of Dynamic Cytosolic Calcium, Sodium, and Potassium Fluxes Following Platelet Activation

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