The effects of hyperosmosis or high pH on a dual‐species biofilm of Enterococcus faecalis and Pseudomonas aeruginosa: an in vitro study

Waal, Suzette V. van der; Sluis, L.W.M. van der; Özok, A.R.; Exterkate, R.A.M.; Marle, J. van; Wesselink, Paul R.; Soet, J.J. de

doi:10.1111/j.1365-2591.2011.01929.x

Cited by 44 publications

(39 citation statements)

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“…1,2 It has been shown that it is impossible to eradicate E. faecalis during treatment, and surviving cells can reside within dentinal tubules and remain viable in root-filled teeth for long periods. 3 The persistence of this species in treated root canals is attributed to its ability to resist the medicaments used during treatment, especially the antibacterial effect of calcium hydroxide, [4][5][6] which is one of the most effective and commonly used endodontic medicaments because of its bactericidal effects derived from its strong alkaline properties. 4 Several mechanisms by which E. faecalis survives extreme alkaline environments have been proposed.…”

mentioning

confidence: 99%

Survival of Enterococcus faecalis during alkaline stress: Changes in morphology, ultrastructure, physiochemical properties of the cell wall and specific gene transcripts

Ran

2013

Archives of Oral Biology

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mentioning

confidence: 99%

Survival of Enterococcus faecalis during alkaline stress: Changes in morphology, ultrastructure, physiochemical properties of the cell wall and specific gene transcripts

Ran

2013

Archives of Oral Biology

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“…, van der Waal et al . ). Moreover, the extreme pH cannot be achieved within dentinal tubules because of the buffering effects of dentine (Haapasalo et al .…”

Section: Introductionmentioning

confidence: 97%

Assessment of dentinal tubule invasion capacity of Enterococcus faecalis under stress conditions ex vivo

et al. 2014

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“…More largely our study highlights the potential use of NaCl as a basic but important component of the therapeutic arsenal used in the management of infectious diseases. Hyperosmotic stress is known for reducing biofilm growth and for being bactericidal on Escherichia coli , P. aeruginosa, and Enterococcus faecalis when NaCl is used at 13% to 35% and could be used for treatment of root canal infection [28] or for locking tunneled hemodialysis catheters [29]. Considering the lower NaCl concentrations used in this study, the antimicrobial effect was probably not related to a direct hyperosmotic lysis.…”

Section: Discussionmentioning

confidence: 79%

Advances toward the Elucidation of Hypertonic Saline Effects on Pseudomonas aeruginosa from Cystic Fibrosis Patients

Michon

Jumas‐Bilak²,

Chiron³

et al. 2014

PLoS ONE

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ObjectivesNebulized hypertonic saline (HTS) has beneficial effects including reducing pulmonary exacerbations in Cystic Fibrosis (CF) patients. Several mechanisms may explain these effects but antimicrobial activity of NaCl remains largely unexplored. We aimed to measure the antimicrobial effect of NaCl on Pseudomonas aeruginosa isolated from the respiratory tract in CF patients.MethodsNaCl minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined for strains characterized for mucoidy, antimicrobial resistance, and ability to form biofilm using 0,9% to 15% NaCl solutions. NaCl effects on biofilm formation, preformed biofilm, and mobility were evaluated. Kinetics of antimicrobial effects was studied.ResultsThe growth of all isolates (n = 85) from 34 patients was inhibited by 6% NaCl solution. A 10% concentration had a bactericidal activity on 90% of the isolates. Mucoid and multidrug resistant (MDR) isolates displayed lower MICs compared to non-mucoid and to non-MDR isolates, respectively. Time-kill kinetics showed that NaCl exhibited a rapid, dose and growth phase dependent bactericidal effect. Three percent or more of NaCl inhibited biofilm formation for 69% of strongly adherent isolates. A dose-dependent decrease of preformed biofilm viability and an inhibitory activity on bacterial motility were observed.ConclusionsNaCl inhibited the growth of all isolates and killed 38% of tested isolates within concentration range currently used in therapeutics. Our results suggest that anti-pseudomonal activity is another mechanism of action of HTS to add to those already established. Clinical trials are needed to compare diverse HTS conditions of use (rhythm, dose and mode of delivery) to obtain efficient and optimized anti-P. aeruginosa effects. More generally, NaCl effect on other opportunistic pathogens as well as on global microbiotae recovered during polymicrobial diseases warrants further investigations.

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The effects of hyperosmosis or high pH on a dual‐species biofilm of Enterococcus faecalis and Pseudomonas aeruginosa: an in vitro study

Abstract: Hyperosmosis effectively reduced a dual-species biofilm of E. faecalis and P. aeruginosa, whilst high pH had limited bactericidal effect in this model.

Cited by 44 publications

References 63 publications

Survival of Enterococcus faecalis during alkaline stress: Changes in morphology, ultrastructure, physiochemical properties of the cell wall and specific gene transcripts

Survival of Enterococcus faecalis during alkaline stress: Changes in morphology, ultrastructure, physiochemical properties of the cell wall and specific gene transcripts

Assessment of dentinal tubule invasion capacity of Enterococcus faecalis under stress conditions ex vivo

Advances toward the Elucidation of Hypertonic Saline Effects on Pseudomonas aeruginosa from Cystic Fibrosis Patients

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